LOCUS       sequence-268335-         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of VP64-ZF37 under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD057 VP64-ZF37
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             121..270
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatca
      121 gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac
      181 cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac
      241 gcccttgatg atttcgacct ggacatgctg attaactcta gaagttccgg atcttctggt
      301 tctggttctg gttcaggatc cggtaccggt ggagctagac ccggggagcg ccccttccag
      361 tgtcgcattt gcatgcggaa cttttcgaga aatttcattt tgcaaagaca tacccgtact
      421 cataccggtg aaaaaccgtt tcagtgtcgg atctgtatgc gaaatttctc cgatagagct
      481 aatttgagga ggcatctgcg tacgcacacc ggcgagaagc cattccaatg ccgaatatgc
      541 atgcgcaact tcagtagaca tgatcaattg actagacacc taaaaaccca cctgagagga
      601 tcttaa
//
