LOCUS       sequence-268355-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of FKBP-ZF43 under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD341 FKBP-ZF43 (NLS)
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(139..141,142..462)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccgtcgac
      121 ggtggactcc aagttaagat gggagtgcag gtggaaacca tctccccagg agacgggcgc
      181 accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga
      241 aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag
      301 gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa
      361 ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca
      421 catgccactc tcgtctttga tgtggagctt ctaaaactgg aaggatccgg tggtggcggg
      481 tctggaggag gaggtagtgg tggaggtggc tctggtaccg ctagacccgg ggagcgcccc
      541 ttccagtgtc gcatttgcat gcggaacttt tcgcgccagg acaggcttga caggcatacc
      601 cgtactcata ccggtgaaaa accgtttcag tgtcggatct gtatgcgaaa tttctcccag
      661 aaggagcact tggcggggca tctacgtacg cacaccggcg agaagccatt ccaatgccga
      721 atatgcatgc gcaacttcag tcgccgcgac aacctgaacc ggcacctaaa aacccacctg
      781 agactcgagt ctagagggcc cgtttaa
//
