LOCUS       sequence-268357-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of VP16-FRB under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD353 VP16-FRB (NLS)
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccgtcgac
      121 ggtggactcc aagccccacc aaccgatgtt tctttaggtg atgagttaca tttggacggt
      181 gaggacgtag ctatggcaca tgcagatgcc ttagacgatt ttgacttgga tatgttagga
      241 gatggagatt caccaggacc tggtttcaca ccacacgatt cagctccata cggtgctttg
      301 gacatggccg atttcgaatt tgaacagatg tttactgatg ccttgggtat agacgaatac
      361 ggtggtggag gatccggtgg tggcgggtct ggaggaggag gtagtggtgg aggtggctct
      421 ggtaccatgc ttgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg
      481 gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg
      541 ggaccccaga ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag
      601 gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cctccaagcc
      661 tgggacctct attatcatgt gttccgacga atctcaaaga ctagatga
//
