LOCUS       sequence-268359-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of VP64-FRB under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD354 VP64-FRB (NLS)
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             121..270
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatca
      121 gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac
      181 cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac
      241 gcccttgatg atttcgacct ggacatgctg attaactcta gaagttccgg atcttctggt
      301 tctggttctg gttcaggatc cggtggtggc gggtctggag gaggaggtag tggtggaggt
      361 ggctctggta ccatgcttga gatgtggcat gaaggcctgg aagaggcatc tcgtttgtac
      421 tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg agcccttgca tgctatgatg
      481 gaacggggac cccagactct gaaggaaaca tcctttaatc aggcctatgg tcgagattta
      541 atggaggccc aagagtggtg caggaagtac atgaaatcag ggaatgtcaa ggacctcctc
      601 caagcctggg acctctatta tcatgtgttc cgacgaatct caaagactag atga
//
