LOCUS       sequence-268363-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of FKBP-ZF43 (no NLS) under the CMV 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD681 FKBP-ZF43 (no NLS)
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(118..120,121..441)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaag gtagtggtat ccatggcgtt cccgtcgacg gtggactcca agttaagatg
      121 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
      181 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      241 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      301 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      361 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtctttgat
      421 gtggagcttc taaaactgga aggatccggt ggtggcgggt ctggaggagg aggtagtggt
      481 ggaggtggct ctggtaccgc tagacccggg gagcgcccct tccagtgtcg catttgcatg
      541 cggaactttt cgcgccagga caggcttgac aggcataccc gtactcatac cggtgaaaaa
      601 ccgtttcagt gtcggatctg tatgcgaaat ttctcccaga aggagcactt ggcggggcat
      661 ctacgtacgc acaccggcga gaagccattc caatgccgaa tatgcatgcg caacttcagt
      721 cgccgcgaca acctgaaccg gcacctaaaa acccacctga gactcgagtc tagagggccc
      781 gtttaa
//
