LOCUS       sequence-268365-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of VP16-FRB (no NLS) under the CMV 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD682 VP16-FRB (no NLS)
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctggtag tggtatccat ggcgttcccg tcgacggtgg actccaagcc
      121 ccaccaaccg atgtttcttt aggtgatgag ttacatttgg acggtgagga cgtagctatg
      181 gcacatgcag atgccttaga cgattttgac ttggatatgt taggagatgg agattcacca
      241 ggacctggtt tcacaccaca cgattcagct ccatacggtg ctttggacat ggccgatttc
      301 gaatttgaac agatgtttac tgatgccttg ggtatagacg aatacggtgg tggaggatcc
      361 ggtggtggcg ggtctggagg aggaggtagt ggtggaggtg gctctggtac catgcttgag
      421 atgtggcatg aaggcctgga agaggcatct cgtttgtact ttggggaaag gaacgtgaaa
      481 ggcatgtttg aggtgctgga gcccttgcat gctatgatgg aacggggacc ccagactctg
      541 aaggaaacat cctttaatca ggcctatggt cgagatttaa tggaggccca agagtggtgc
      601 aggaagtaca tgaaatcagg gaatgtcaag gacctcctcc aagcctggga cctctattat
      661 catgtgttcc gacgaatctc aaagactaga tga
//
