LOCUS       sequence-268367-         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of VP64-FRB (no NLS) under the CMV 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD683 VP64-FRB (no NLS)
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             106..255
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctggtag tggtatccat ggcgttcccg gatcagacgc attggacgat
      121 tttgatctgg atatgctggg aagtgacgcc ctcgatgatt ttgaccttga catgcttggt
      181 tcggatgccc ttgatgactt tgacctcgac atgctcggca gtgacgccct tgatgatttc
      241 gacctggaca tgctgattaa ctctagaagt tccggatctt ctggttctgg ttctggttca
      301 ggatccggtg gtggcgggtc tggaggagga ggtagtggtg gaggtggctc tggtaccatg
      361 cttgagatgt ggcatgaagg cctggaagag gcatctcgtt tgtactttgg ggaaaggaac
      421 gtgaaaggca tgtttgaggt gctggagccc ttgcatgcta tgatggaacg gggaccccag
      481 actctgaagg aaacatcctt taatcaggcc tatggtcgag atttaatgga ggcccaagag
      541 tggtgcagga agtacatgaa atcagggaat gtcaaggacc tcctccaagc ctgggacctc
      601 tattatcatg tgttccgacg aatctcaaag actagatga
//
