LOCUS       sequence-268369-        2016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of VPR-FRB (no NLS) under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD684 VPR-FRB (no NLS)
FEATURES             Location/Qualifiers
     source          1..2016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             106..255
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             712..1068
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             1087..1656
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctggtag tggtatccat ggcgttcccg gatcagacgc attggacgat
      121 tttgatctgg atatgctggg aagtgacgcc ctcgatgatt ttgaccttga catgcttggt
      181 tcggatgccc ttgatgactt tgacctcgac atgctcggca gtgacgccct tgatgatttc
      241 gacctggaca tgctgattaa ctctagaagt tccggatctg ttggtagcca gtacctgccc
      301 gacaccgacg accggcaccg gatcgaggaa aagcggaagc ggacctacga gacattcaag
      361 agcatcatga agaagtcccc cttcagcggc cccaccgacc ctagacctcc acctagaaga
      421 atcgccgtgc ccagcagatc cagcgccagc gtgccaaaac ctgcccccca gccttacccc
      481 ttcaccagca gcctgagcac catcaactac gacgagttcc ctaccatggt gttccccagc
      541 ggccagatct ctcaggcctc tgctctggct ccagcccctc ctcaggtgct gcctcaggct
      601 cctgctcctg caccagctcc agccatggtg tctgcactgg ctcaggcacc agcacccgtg
      661 cctgtgctgg ctcctggacc tccacaggct gtggctccac cagcccctaa acctacacag
      721 gccggcgagg gcacactgtc tgaagctctg ctgcagctgc agttcgacga cgaggatctg
      781 ggagccctgc tgggaaacag caccgatcct gccgtgttca ccgacctggc cagcgtggac
      841 aacagcgagt tccagcagct gctgaaccag ggcatccctg tggcccctca caccaccgag
      901 cccatgctga tggaataccc cgaggccatc acccggctcg tgacaggcgc tcagaggcct
      961 cctgatccag ctcctgcccc tctgggagca ccaggcctgc ctaatggact gctgtctggc
     1021 gacgaggact tcagctctat cgccgatatg gatttctcag ccttgctggg ctctggcagc
     1081 ggcagccggg attccaggga agggatgttt ttgccgaagc ctgaggccgg ctccgctatt
     1141 agtgacgtgt ttgagggccg cgaggtgtgc cagccaaaac gaatccggcc atttcatcct
     1201 ccaggaagtc catgggccaa ccgcccactc cccgccagcc tcgcaccaac accaaccggt
     1261 ccagtacatg agccagtcgg gtcactgacc ccggcaccag tccctcagcc actggatcca
     1321 gcgcccgcag tgactcccga ggccagtcac ctgttggagg atcccgatga agagacgagc
     1381 caggctgtca aagcccttcg ggagatggcc gatactgtga ttccccagaa ggaagaggct
     1441 gcaatctgtg gccaaatgga cctttcccat ccgcccccaa ggggccatct ggatgagctg
     1501 acaaccacac ttgagtccat gaccgaggat ctgaacctgg actcacccct gaccccggaa
     1561 ttgaacgaga ttctggatac cttcctgaac gacgagtgcc tcttgcatgc catgcatatc
     1621 agcacaggac tgtccatctt cgacacatct ctgttttctg gttctggttc tggttcagga
     1681 tccggtggtg gcgggtctgg aggaggaggt agtggtggag gtggctctgg taccatgctt
     1741 gagatgtggc atgaaggcct ggaagaggca tctcgtttgt actttgggga aaggaacgtg
     1801 aaaggcatgt ttgaggtgct ggagcccttg catgctatga tggaacgggg accccagact
     1861 ctgaaggaaa catcctttaa tcaggcctat ggtcgagatt taatggaggc ccaagagtgg
     1921 tgcaggaagt acatgaaatc agggaatgtc aaggacctcc tccaagcctg ggacctctat
     1981 tatcatgtgt tccgacgaat ctcaaagact agatga
//
