LOCUS       sequence-268371-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of VP64-FRB (NES) under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD880 VP64-FRB (NES)
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             148..297
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataacg gtatggagga actttcacag gccctggcta gtagtttttc agtgtctggt
      121 gtcggtatcc atggcgttcc cggatcagac gcattggacg attttgatct ggatatgctg
      181 ggaagtgacg ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac
      241 tttgacctcg acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt
      301 aactctagaa gttccggatc ttctggttct ggttctggtt caggatccgg tggtggcggg
      361 tctggaggag gaggtagtgg tggaggtggc tctggtacca tgcttgagat gtggcatgaa
      421 ggcctggaag aggcatctcg tttgtacttt ggggaaagga acgtgaaagg catgtttgag
      481 gtgctggagc ccttgcatgc tatgatggaa cggggacccc agactctgaa ggaaacatcc
      541 tttaatcagg cctatggtcg agatttaatg gaggcccaag agtggtgcag gaagtacatg
      601 aaatcaggga atgtcaagga cctcctccaa gcctgggacc tctattatca tgtgttccga
      661 cgaatctcaa agactagatg a
//
