LOCUS       sequence-26842-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Peterson TR, Laplante M, Thoreen CC, Sancak Y, Kang SA, Kuehl WM, 
            Gray NS, Sabatini DM
  TITLE     DEPTOR is an mTOR inhibitor frequently overexpressed in multiple 
            myeloma cells and required for their survival.
  JOURNAL   Cell. 2009 May 29;137(5):873-86. doi: 10.1016/j.cell.2009.03.046. 
            Epub 2009 May 14.
  PUBMED    19446321
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.03.046"; journalName: "Cell"; date: 
            "2009-05-29- 29"; volume: "137"; issue: "5"; pages: "873-86"
COMMENT     Sequence Label: pLKO mouse shRNA 2 raptor
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    147..264
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..823
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     742..760
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(845..864)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cgncgattcg attcttggct ttanatatct tgtggaaagg acgaaacacc gggcccgagt
       61 ctgtgaatgt aatctcgaga ttacattcac agactcgggc tttttgaatt ctcgacctcg
      121 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
      181 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      241 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
      301 ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
      361 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
      421 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
      481 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
      541 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
      601 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
      661 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
      721 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
      781 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggagct
      841 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccaggc
//
