LOCUS       sequence-26844-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Peterson TR, Laplante M, Thoreen CC, Sancak Y, Kang SA, Kuehl WM, 
            Gray NS, Sabatini DM
  TITLE     DEPTOR is an mTOR inhibitor frequently overexpressed in multiple 
            myeloma cells and required for their survival.
  JOURNAL   Cell. 2009 May 29;137(5):873-86. doi: 10.1016/j.cell.2009.03.046. 
            Epub 2009 May 14.
  PUBMED    19446321
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.03.046"; journalName: "Cell"; date: 
            "2009-05-29- 29"; volume: "137"; issue: "5"; pages: "873-86"
COMMENT     Sequence Label: pLKO human shRNA 1 DEPTOR
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    144..261
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        310..821
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     739..757
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 cnnncgatcg attctggctt ntatatcttg tggaaggacg aaacaccggg ccatgacaat
       61 cggaaatcta ctcgagtaga tttccgattg tcatggcttt ttgaattctc gacctcgaga
      121 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
      181 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      241 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
      301 cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
      361 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
      421 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
      481 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
      541 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
      601 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
      661 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggngcgg tagtgtgggc
      721 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
      781 cggctccctc gttgaccgaa tcaccgaccc tctctcccca gggggatcca ccggagctta
      841 ccatgaccga gtacaagccc ncggtgcgcc tcnccnnccn ccga
//
