LOCUS       sequence-26849-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Jiang H, Vogt PK
  TITLE     Constitutively active Rheb induces oncogenic transformation.
  JOURNAL   Oncogene. 2008 Sep 25. 27(43):5729-40.
  PUBMED    18521078
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2008 Sep 25. 27(43):5729-40."
COMMENT     Sequence Label: RCAS(B)-Rheb-Q64L
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(385..408)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      408..417
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gaccttcttg cggtctttct tattcccaac caacataata ggtatttgta ctttccccac
       61 catatccaac aatttgccat ggataacttt aatcacttca aaacttttga ttgatgtaac
      121 agaatacaca agaatatagc cattaatatc tatggagtat gtctgaggaa agatagaata
      181 ttcatctagc ccggctgtgt ctacaagttg aagatgatat tcttgtccat ttactgtgat
      241 caactttgta aaagtgtttt ctatggttgg atcgtaggag tccacaaatt ggccttcaac
      301 aaattgaatc gtcaatgagg atttccccac agaccggtag cccaggatcg cgatcttccg
      361 ggacttggac tgcggcgcgg ccgccttgtc atcgtcatcc ttgtagtcca tggtggcccg
      421 cggccgtaat ggcccgatgc cacagtggta cgcgaggcca ccagcagagt cagctcagct
      481 cctgcagcac acagacaaaa gcgtatttca caattaccaa tagctgtctt tcctgccttc
      541 ctcgatggac tcgcaagcgc tataccacca cccatgtact gccttatact attctgcttt
      601 caggctgccc acaggccttt tgcagcttct tatattcntn ggggtggtag ctgagaggag
      661 ttataancca cnntctgang ttaccgcata ccattgcaaa ggcntgggag gcacactact
      721 agcacaaata ctacagcccn aagcagtcct tcacaatgac ggccatctct atcccgatag
      781 ccnccagcac tccattggcc tgtcnccgac tatgntgtct ctacngactc tcgnaaccng
      841 gacncatgan ccatcgnnnc ncactgctan agaaannacn cgtcngcggc acgancaanc
      901 angtgatcna gagtcttagg cncggaatgc
//
