LOCUS       sequence-268548-       11287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK1 (human) tagged with N-terminal V5
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11287)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-BRSK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..11287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2133..2153
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2153..3364
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3201..3220
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3368..3388)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             3392..3660
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3670..3711
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3709..3733
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6074..6098)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6128..6716
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6181..6201)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6599..6610)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6730..7229
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6765..6788)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7162..7181
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7250..7849
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7250..7269)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7746..7766
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7987..8220
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8292..8413
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8329..8348)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8383..8402
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8453..8588
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8608..8627)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8609..8627)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8637..8654)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8637..8653)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8646..8668)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8795..9250
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8882..8901)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9092..9113
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9276..9380
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9381..9449,9450..10241)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9599..9618)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10412..11000
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10901..10920
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11154..11171
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg
     2161 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     2221 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     2281 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     2341 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     2401 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     2461 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     2521 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     2581 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     2641 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     2701 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     2761 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     2821 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     2881 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     2941 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     3001 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     3061 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     3121 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     3181 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     3241 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     3301 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     3361 agacggccaa cttttgtata caaagtggcg aggggctcgc atctctcctt cacgcgcccg
     3421 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     3481 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     3541 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     3601 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     3661 ggcaccatgg gtaagcctat ccctaaccct ctcctcggtc tcgattctac aactttgtac
     3721 aaaaaagcag gcttaatgtc gtccggggcc aaggagggag gtgggggctc tcccgcctac
     3781 cacctccccc acccccaccc ccacccaccc cagcacgccc aatatgtggg cccctatcgg
     3841 ctggagaaga cgctgggcaa aggacagaca gggctggtta aactcggggt ccactgcatc
     3901 acgggtcaga aggtcgccat caagatcgtg aaccgggaga agctgtcgga gtcggtgctg
     3961 atgaaggtgg agcgggagat cgccatcctg aagctcatcg aacacccaca tgtcctcaag
     4021 ctccacgacg tctacgagaa caagaaatat ttgtacctgg ttctggagca cgtctcgggg
     4081 ggtgagctat tcgactacct ggtaaagaag gggagactga cgcccaagga ggcccgaaag
     4141 ttcttccgcc agattgtgtc tgcgctggac ttctgccaca gctactccat ctgccacaga
     4201 gacctaaagc ccgagaacct gcttttggat gagaaaaaca acatccgcat tgcagacttc
     4261 ggcatggcgt ccctgcaggt gggggacagc ctcctggaga ccagctgcgg gtccccccat
     4321 tatgcgtgtc cagaggtgat taagggggaa aaatatgatg gccgccgggc agacatgtgg
     4381 agctgtggag tcatcctctt cgccctgctc gtgggggctc tgccctttga tgacgacaac
     4441 ctccgccagc tgctggagaa ggtgaaacga ggcgtcttcc acatgcccca cttcattcct
     4501 ccagattgcc agagcctcct gaggggaatg atcgaagtgg agcccgaaaa aaggctcagt
     4561 ctggagcaaa ttcagaaaca tccttggtac ctaggcggga aacacgagcc agacccgtgc
     4621 ctggagccag cccctggccg ccgggtagcc atgcggagcc tgccatccaa cggagagctg
     4681 gaccccgacg tcctagagag catggcatca ctgggctgct tcagggaccg cgagaggctg
     4741 catcgcgagc tgcgcagtga ggaggagaac caagaaaaga tgatatatta tctgcttttg
     4801 gatcggaagg agcggtatcc cagctgtgag gaccaggacc tgcctccccg gaatgatgtt
     4861 gacccccccc ggaagcgtgt ggattctccc atgctgagcc gtcacgggaa gcggcgacca
     4921 gagcggaagt ccatggaagt cctgagcatc accgatgccg ggggtggtgg ctcccctgta
     4981 cccacccgac gggccttgga gatggcccag cacagccaga gatcccgtag cgtcagtgga
     5041 gcctccacgg gtctgtcctc cagccctcta agcagcccaa ggagtccggt cttttccttt
     5101 tcaccggagc cgggggctgg agatgaggct cgaggcgggg gctccccgac ttccaaaacg
     5161 cagacgctgc cttctcgggg ccccaggggt gggggcgccg gggagcagcc cccgcccccc
     5221 agtgcccgct ccacacccct gcccggcccc ccaggctccc cgcgctcctc tggcgggacc
     5281 cccttgcact cgcctctgca cacgccccgg gccagtccca ccgggacccc ggggacaaca
     5341 ccacccccca gccccggcgg tggcgtcggg ggagccgcct ggaggagtcg tctcaactcc
     5401 atccgcaaca gcttcctggg ctcccctcgc tttcaccggc gcaagatgca ggtccctacc
     5461 gctgaggaga tgtccagctt gacgccagag tcctccccgg agctggcaaa acgctcctgg
     5521 ttcgggaact tcatctcctt ggacaaagaa gaacaaatat tcctcgtgct aaaggacaaa
     5581 cctctcagca gcatcaaagc agacatcgtc catgcctttc tgtcgatccc cagcctgagt
     5641 cacagtgtgc tgtcacagac cagcttcagg gccgagtaca aggccagtgg cggcccctcc
     5701 gtcttccaaa agcccgtccg cttccaggtg gacatcagct cctctgaggg tccagagccc
     5761 tccccgcgac gggacggcag cggaggtggt ggcatctact ccgtcacctt cactctcatc
     5821 tcgggtccca gccgtcggtt caagcgagtg gtggagacca tccaggcaca gctcctgagc
     5881 actcatgacc agccctccgt gcaggccctg gcagacgaga agaacggggc ccagacccgg
     5941 cctgctggtg ccccaccccg aagcctgcag cccccacccg gccgcccaga cccagagctg
     6001 agcagctctc cccgccgagg cccccccaag gacaagaagc tcctggccac caacgggacc
     6061 cctctgccct gaaacccagc tttcttgtac aaagttgtga tatccagcac agtggcggcc
     6121 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6181 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6241 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6301 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6361 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6421 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6481 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     6541 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6601 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6661 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     6721 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     6781 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     6841 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     6901 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     6961 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     7021 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     7081 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     7141 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     7201 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta
     7261 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     7321 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     7381 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     7441 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     7501 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     7561 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     7621 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     7681 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     7741 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     7801 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     7861 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac
     7921 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7981 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg
     8041 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8101 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8161 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8221 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     8281 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8341 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8401 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     8461 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8521 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8581 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     8641 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8701 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8761 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     8821 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8881 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8941 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9001 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9061 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9121 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9181 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     9241 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9301 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9361 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9421 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9481 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9541 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     9601 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     9661 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9721 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9781 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9841 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9901 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9961 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10021 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10081 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10141 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10201 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10261 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10321 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10381 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10441 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10501 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10561 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10621 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    10681 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10741 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10801 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10861 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10921 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10981 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    11041 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    11101 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    11161 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    11221 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    11281 acgcaat
//
