LOCUS       sequence-268552-       12196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding Marburg virus L gene under 
            control of the AG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Manhart WA, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Muhlberger 
            E
  TITLE     A Chimeric Lloviu Virus Minigenome System Reveals that the 
            Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than
            Marburgviruses.
  JOURNAL   Cell Rep. 2018 Sep 4;24(10):2573-2580.e4. doi: 
            10.1016/j.celrep.2018.08.008.
  PUBMED    30184492
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.08"; journalName: "Cell Rep"; date: 
            "2018-09-4- 4"; volume: "24"; issue: "10"; pages: "2573-2580.e4"
COMMENT     Sequence Label: pCAGGS_L_MARV
FEATURES             Location/Qualifiers
     source          1..12196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             108..122
                     /estimated_length=15
     intron          275..1291
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1215..1237
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1299..1318
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(8829..8848)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8894..8949
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8948..8967)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9310..9326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9310..9326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9323..9345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9334..9350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9358..9364,9365..9382,9383..9388))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9403..9424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9483..9678
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9529..9664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9684..9818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9721..9740)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9775..9794
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9886..9903)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10057..10645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10137..10156)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10816..11607,11608..11676))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11439..11458
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11677..11781)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        11812..12191
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
       61 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggnnn nnnnnnnnnn
      121 nncgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      181 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      241 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
      301 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
      361 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
      421 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
      481 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
      541 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
      601 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
      661 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
      721 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
      781 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
      841 ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc
      901 gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
      961 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     1021 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     1081 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     1141 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     1201 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     1261 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     1321 gtctcatcat tttggcaaag aattcctcga gggtaccccc ggggcggccg ctcaacatgc
     1381 agcatccaac tcaatatcct gatgcaaggt tgtcctctcc tataatccta gaccagtgtg
     1441 acttattagc cagaagttta gggttgtata gtcattattc acataatccg aaattgcgta
     1501 attgtaggat tccacatcac atttaccgtt taaggaattc gacagcatta aagacatttc
     1561 ttcagaattg ttcgatactc accgttcctt ttcattcaat ttgggatcat attttaactt
     1621 ccattcaata tgatgcaatt aatcatgttg atgattttaa atacctattg ccatctgagc
     1681 tagtcaagta tgcgaattgg gacaacgagt tcttaaaagc atatcttaat aagatcttag
     1741 gacttgacca tgttttttca gcttctgcaa ggtcacagtg tgaggatttt tctcctaagg
     1801 aaaatcctta ttattggggg atgttattac tcgtgcatct atctcaactt gccaggagga
     1861 taaaaggaca aagagggtca ttaagaagta actggaaatt tataggaaca gatttggagc
     1921 tgtttggaat agcagatttt gttattttta aggttccagt aaaaacaata atccgaaatg
     1981 ctgtaagctt acaagcttca aaaccgggat taagaatatg gtaccgtgac caaaacttga
     2041 ccccttatct atgcgacgat gagtttattg taagcgtcgc tagttatgaa tgttttatca
     2101 tgattaaaga cgtcttcatt gagaggtata acacatggga aatctgtgcc cgcgcttggc
     2161 tcgaagacag tgatggagct gattatcctc ctcttgatgt gttaggtgaa ttatacaacc
     2221 agggagacca aattattgcc atgtacctgg aggacggttt caaattgatt aaacacttag
     2281 aacccttgtg tgtcagctgt atacaaacac atggcatctt tacaccaaga aaatactggt
     2341 tccaatcaca gatgattaag tcatattatg atgaactcca tgatctcaat ttgaaacttc
     2401 aaatttcaga caataaggct gagtgtgccc aaaactttat taaaactata gttcaggcga
     2461 aattgactcc tcaacaatac tgtgaattat tctccctaca aaagcattgg ggtcatcccg
     2521 ttttatacaa tgatgttgca ctagataagg ttaaaaaaca tgcgcaatcg acaaaaatct
     2581 taaaacctaa agtcatgttt gaaacttttt gtgttttcaa atttatagta gcaaagaatc
     2641 attatcattc tcaaggatca tggtataaaa ccacacatga tttgcatttg actccatatc
     2701 ttagacaaca tattgtgtca aattcatttc catcacaagc cgaaatttat cagcatcttt
     2761 gggagtggta tttcgtggag catgaacctc ttttctcaac taaaataata agtgatttaa
     2821 gtatctttat aaaagacagg gctaccgctg tgaaccagga gtgttgggac agtgtcttcg
     2881 atagaagtgt attaggatat aaccctcctg ttagatttca atcaaagaga gtgccagagc
     2941 aatttttggg tcaagcagac ttttccttga atcaaatatt agagtttgct gaaaagttag
     3001 agtatttggc tccttcttat aggaattttt ccttctcatt aaaagaaaaa gagttgaata
     3061 taggaagaac ttttgggaag ttaccgtatc gtgtcagaaa tgtccaaaca ctcgcagaag
     3121 ccctgctagc agatggactg gcaaaagcat tccctagtaa catgatggtt gtcactgaga
     3181 gggaacagaa agaagcatta ttacatcagg cttcttggca ccacaattca gcaagcatag
     3241 gggagaacgc tatagtgagg ggtgcaagtt ttgttactga tcttgagaaa tacaacctcg
     3301 ccttccgata tgaatttaca cggcatttca tagactactg taatcgatgt tatggtgtga
     3361 agaatttatt tgattggatg cattttttaa taccactatg ttatatgcat gtcagtgact
     3421 tttatagccc accacattgt gtgacagaag ataatcgaaa taacccacct gattgtgcta
     3481 atgcttatca ttatcactta ggaggtatag agggacttca gcagaaattg tggacatgca
     3541 tatcatgtgc ccaaatcacc cttgtggagt taaaaactaa attaaaattg aagtccagcg
     3601 tcatgggtga taatcaatgt ataacaactc taagtctttt tccaattgat gctcccaacg
     3661 attatcaaga gaacgaggct gaattgaatg cggcacgagt tgctgtcgaa ttagctatta
     3721 ctacaggtta tagtggtata tttttaaagc ctgaggaaac atttgtccat tcagggttca
     3781 tttattttgg taaaaagcaa tatctcaacg gtgttcaact gccgcaatca ttgaaaacaa
     3841 tggcaaggtg tggaccctta tctgactcta ttttcgatga tcttcaaggt tctctggcca
     3901 gtattggtac atcctttgag agaggaacaa gcgagacacg gcacattttt ccgagccgtt
     3961 ggatagcttc attccattca atgttagcaa taaatttatt aaatcagaat caccttgggt
     4021 ttcctctagg gttcaatatt gatatttctt gtttcaaaaa gcctcttacc ttctcggaaa
     4081 aattaattgc tctcataacg ccccaagttt taggagggtt atcattttta aatccagaaa
     4141 aattgttcta ccggaacata agtgatcctc tcacttcagg tctatttcaa ctcaagaatg
     4201 cattggaatt tcttgaaaag gaagaattat tctatatctt gatttctaaa aaacctggtt
     4261 tagcagatgc ctcagatttt gtcatgaatc cattaggctt aaatgtacca ggatcaaagg
     4321 aaataataac gttccttaga caaacagttc gcgaaaatat cacgatcacg tcacaaaata
     4381 gaataataaa ttctcttttc cacataggtt ctgatttaga ggaccaaaga gtgtgtgagt
     4441 ggcttttatc atcaaaccct gtaatgagcc gatttgctgc tgacatcttt tcaagaacac
     4501 ctagtggaaa acggcttcaa gtcttaggct atctagaagg aacaagaaca ttactagctt
     4561 ctcggacaat cagtttaact acagaaggaa caatgttgat gaaattaaga gaattaacga
     4621 gaaaccgatg gaaaagttgg ttttcttata ttgatgcact ggacgatgat ttatctgagt
     4681 ccttggaaaa gttcacatgt actgttgatg tggctaattt cttgagggca tattcatggt
     4741 ctgacgtctt aaaagggaaa aggctaattg gtgccacact gccatgttta ctagagcaat
     4801 ttgaggtaaa gtggattaat ttatctgagg atttaaggga acaatttaat ctatcttcag
     4861 actcaaaatc aactataaac ttgttgccgt atgactgtaa ggaactgcga cttgaaggaa
     4921 gcaatgacac agagttaaat tatgtcagtt gtgctcttga ccggaaagtt gtccagaaac
     4981 atccctctgt taatcgtcta gcttggacga taggaaatcg agcaccgtat attggctcac
     5041 ggacagaaga taagatcggt tatcctccct taagagtaaa ttgcccatca gcagcactta
     5101 aagaagctat tgagatggtt tctagattgt tatgggtgac tcaaggcact gcagaccgag
     5161 aaaaattgct tattcctctt ctcaattcaa gagtaaatct ggactatcag acggtgctta
     5221 actttttacc tacacactac tcaggcaaca tagttcatag atataatgat caatatggac
     5281 aacattcctt tatggcaaac aggatgagta atacatctac acgtgcaatt atatcaacta
     5341 acacactggg taaatatgct gggggaggtc aagctgctat tgatagtaat ataatctttc
     5401 aaaatactat taatttagga gttgcagttt tagatattgc attgtctctt gctaaattgt
     5461 cgtcagcatc aaatgtcact ttccgtttaa tgttaaataa gtgctgcacg cggcatgtac
     5521 cgtccgaata cctatatttt gataaacctt tagatgtgga tttgaacaag tatatggaca
     5581 atgagttggt ttatgacaat gaccctcttt gcagtgggat taaagggaga ttaggcagag
     5641 tatctcgatc aacactcaca ttgagtttga atgtcagtga cattggttct tatgactttc
     5701 caactattgc tgcatggaca ctaggagaaa ctatagtcgg aagcattttt tctgatgaat
     5761 cttctcaaag tacggatcca ataagctcag gttgcacaaa aactttcgtc acacatttcc
     5821 ttgtgtatcc agttgagagt atattttatg cattcggagc taacttaata gttgaaagtt
     5881 taagtctaag taggatcaaa tcaattaaga acctctcaga tttgacattc cttatatcat
     5941 ccacaatcag gaatttatca catagatcac ttcggattct tcaatctact ttccgacatg
     6001 aattggtgct cacccgacta gcccaccaca taccgttaat ttctttaatg ttagggggct
     6061 ctgcaggaga gaagagttca tcagatgctg ttcggctatt tcttacagca agttatcaga
     6121 attttatcaa taatttcagt tgtctgatga agaagggtca gtcatcgcta ccggtttggc
     6181 tttactttcc tagtgaaggg caacaattaa agcctatatt aaaaatctta cagagattat
     6241 cagacttgtt atcacctgac aaaattcaaa agcgtaaaat tttggctgac acctgttgtc
     6301 caattggcag cttttgggtc tatccaagca agtccacaag gactaaccat tattatgcaa
     6361 gccttaatta ttggagagac aaagctaata aggttaagaa tactcctttt tcacacttga
     6421 taaattgttc atttcctgaa ttttcttcac ataccagttc agtctcctct aatcaacaag
     6481 tgaccaattc gaagtatatt gtttatccag aaaatatcac tgaaataaat gcaagaacca
     6541 gattaataaa ttatggatca acagctctac aggggatgga caccaagatg ccactctcag
     6601 agcaaaatct agtcgaaaat tgtcgaccat cagagggcat cagattcaag gacaatcaaa
     6661 aaataacaaa acatgaccag agatgtgaga gggaggaatc ttcaccgcaa cagatgttcc
     6721 ctgaagataa catgcagact cctgcgcaca tacatagttc ctccccattt caaatcctta
     6781 taaaatcact agatgcacat gaggactttg atgcctcgaa gataatctta aattctgaaa
     6841 taaataatct taaccttacg gagtatactc ttaatacaaa gttattgaca actcctacca
     6901 ggacagaaat tttagataca agtccgttac aatcctctag atattcatca acttccaggg
     6961 aacggtctct actatccaga gaacaagctt catatttgta cgttgattgc agtaatattc
     7021 cttctatctc tctagaccca ggttttcgga gtatgtctga tcagaatcaa gttcaaatgt
     7081 taatcaatac ctacaaacgt gatttacatg cttgttttga tagcaatcaa ttctgtcggt
     7141 ttacaggggt agtctcatca atgcattaca agctttatga tcttttgcct ccaggtaaat
     7201 tgaaaaaggc aatttgtttg gccgaagggg aaggaagtgg tgctcggtta cttttgaagt
     7261 ggaaggaaac ggattattta ttcttcaaca ctttggctac ggattcacaa caagaagccg
     7321 agattttgag tggccgggta ataccgagaa tgttgtataa catagacaga ttaagtgctt
     7381 tgcttgaatc aaggagacta atattgaaca acctaactat ccaaattaca gatattacaa
     7441 atccattatg gctagattct gtaatacaat atttacctga agatagtgac attcttacaa
     7501 tggacgcaga gaccaccaag gatgaaacaa gggaacagct ttataaaact attgtgaata
     7561 tttggacacg tacttctcct aatatcccaa aaattagcat catcaaggta tttttattag
     7621 actatgaagg gactttattc ttaatgaaga atgctattca gtattatggg caagttcaac
     7681 tcaagaaacc atatagctca aatgcaaaaa actcagaatg gtacttgtgt tgcggtaaac
     7741 gaagaattca acggcttcaa attgatttct cagaccaggt gggaattttt ctgatttgta
     7801 aagcaatgtc acgccaaaga caagcaattc cttactggtt aaaacatata gaaaagaatt
     7861 atcctgcttc attacacgag tttttcctaa ctttgggttt cccttcttta gagtcatctt
     7921 tctgccatcg ttatactatt ccattcagtg aaggaaaggc tctttttcac aaggtccagt
     7981 cttatgttcg tcaaggcaaa caacatttac attctcttat gttggattat gaaaacaatt
     8041 cacctctact agacttgaga aatcacttta tttgctcatt aaggggaaag ataactaaat
     8101 attacaatga tatattaaag ttaaatctag tcatcaaggc agtagaaaaa ggtaaaaatt
     8161 ggtcacaact tgttgagatc cttcctaata tgcattcagt atgcatagtg cacgtggatc
     8221 atgagtgttc tggatgcgag aaacggttat tacttaaatt ggattttatc agaaatacaa
     8281 agatcgcaga acaaaaatta cttaacagag taatcgggta tatcctattc tttccattcg
     8341 gtctgtttaa atctggatca ttaagggcat aatttcaaca gagagaactt catttaattc
     8401 acaaaaacaa tctatttaag agtgagggtt acattgtcta agatattgta tgagaagtaa
     8461 taaaataaat aagaaaacga aaagactatt agacagctta ttttatacaa gataatctta
     8521 tatcgcttta ggcctcacac aagtgagaaa attacgcgca cagattaact agtgattaag
     8581 tgtttggtca caccagaggt aactttttaa cgttaattac tcagatgtta ttgctcataa
     8641 ttagcattaa tattggcaca ttgggtgaat ccttgagctt tatccctaat atggtgtaag
     8701 aaattaagga aatactgaga tacactagtt gaattgaatt atgacatacc atatatcata
     8761 aatataaaaa agtgtctgct gtaatctaca agcacctcgc ggccgcgagc tcttaattag
     8821 ctagcagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     8881 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     8941 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     9001 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     9061 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     9121 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     9181 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     9241 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
     9301 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9361 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9421 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     9481 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9541 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9601 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9661 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
     9721 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9781 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
     9841 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9901 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9961 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10021 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10081 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10141 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10201 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10261 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10321 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10381 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10441 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10501 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10561 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10621 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10681 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10741 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10801 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10861 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10921 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10981 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11041 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11101 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11161 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11221 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11281 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11341 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11401 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    11461 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11521 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11581 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11641 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11701 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11761 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat
    11821 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    11881 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    11941 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    12001 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    12061 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    12121 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    12181 ctattaccat ggtcga
//
