LOCUS       sequence-268553-        6185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Marburg virus VP35 gene under control of the AG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Schmidt KM, Schumann M, Olejnik J, Krahling V, Muhlberger E
  TITLE     Recombinant Marburg virus expressing EGFP allows rapid screening of 
            virus growth and real-time visualization of virus spread.
  JOURNAL   J Infect Dis. 2011 Nov;204 Suppl 3:S861-70. doi: 
            10.1093/infdis/jir308.
  PUBMED    21987762
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Infect 
            Dis."; date: "2011-11"; volume: "204 Suppl 3:S861-70. doi"; pages: "
            10.1093/infdis/jir308"
COMMENT     Sequence Label: pCAGGS_VP35_MARV
FEATURES             Location/Qualifiers
     source          1..6185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..285
                     /label=chicken beta-actin promoter
     intron          286..1302
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1226..1248
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1310..1329
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2834..2853)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2899..2954
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2953..2972)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3315..3331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3315..3331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3328..3350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3339..3355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3363..3369,3370..3387,3388..3393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3408..3429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3488..3683
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3534..3669
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3689..3823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3726..3745)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3780..3799
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3891..3908)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4062..4650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4142..4161)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4821..5612,5613..5681))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5444..5463
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5682..5786)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(5817..6185,1..11)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
       61 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcng
      121 gggggggggs gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      181 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      241 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc
      301 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
      361 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
      421 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
      481 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
      541 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
      601 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
      661 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
      721 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
      781 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
      841 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
      901 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
      961 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     1021 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     1081 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     1141 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     1201 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1261 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1321 gttattgtgc tgtctcatca ttttggcaaa gaattccccg gggagctcac tagtggatcc
     1381 ctcacaatgt gggactcatc atacatgcag caagtcagcg aagggttgat gactggaaaa
     1441 gtacccatag atcaagtgtt tggtgccaat cccttagaga agttatacaa gagaagaaaa
     1501 ccaaaaggca cagttggact acaatgtagc ccttgtctaa tgtcaaaggc gacaagtact
     1561 gatgatatta tttgggacca actgatcgtg aagagaacac tagctgatct acttataccg
     1621 ataaataggc agatatcaga cattcaaagc actctaagcg aagtaacaac aagagtccat
     1681 gaaattgagc ggcaattaca tgagattacc ccagttttaa aaatgggaag gacactggaa
     1741 gcaatttcca aggggatgtc agaaatgtta gccaaatacg accaccttgt aatttcaact
     1801 ggaagaacca ctgcaccagc tgctgccttt gatgcctact taaatgagca tggtgtccct
     1861 ccccctcaac ccgcgatttt caaagatctt ggggttgccc aacaagcttg tagtaagggg
     1921 accatggtta aaaatgcaac aacagatgca gccgacaaga tgtcgaaggt tcttgaactc
     1981 agtgaggaaa cgttctccaa gccaaacctt tcagctaagg atttagccct tttattgttt
     2041 acccatctac ccggcaacaa cactccattc catatcctag ctcaggtcct ttcaaaaatt
     2101 gcttacaagt caggaaaatc cggagcattc ttggatgcat ttcaccagat tctaagtgaa
     2161 ggagagaatg ctcaggcggc attaactcga ctaagcagaa catttgacgc tttccttgga
     2221 gtggttcctc cagtgataag agtcaaaaac ttccaaacag tccctcgtcc atgtcaaaaa
     2281 agtcttcggg ctgtccctcc aaatccaaca attgacaaag gatgggtctg tgtttattca
     2341 tctgagcaag gtgaaacacg ggcccttaaa atctaattct cattgttcat agttgcaagg
     2401 gaagtgatct ttccgagttg atacaaagac actaaacatt tcaaaagcat gtatgtggac
     2461 aaaacataat tagaccatct taattggagt agtaatttat ttctgtctta aatgtgattt
     2521 tcactttaaa agcgttaaat ggtgatagat taatccttga agttactctt ctatatatta
     2581 tagagaaacc aatgttacta acaaaagggg tctacctaac gcatatgatt gagtaatccg
     2641 tatattttat aaaccaaaca attaacttct tactttttaa gaatcaacta acaacataga
     2701 aaagacattt atccttatgt aatcctcggc ttagttgaaa ttaacttttg ttggacctca
     2761 agacgcttat tcatagtatg gatccctgca gctcgagagg ggggcggccg cgagctctta
     2821 attagctagc agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     2881 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     2941 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     3001 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     3061 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     3121 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     3181 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     3241 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
     3301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3481 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3541 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3601 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3661 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
     3721 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3781 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
     3841 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3901 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3961 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4021 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4081 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4141 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4201 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4261 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4321 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4381 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4441 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4501 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4561 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4621 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4681 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4741 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4801 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4861 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4921 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4981 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5041 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5101 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5161 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5221 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5281 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5341 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5401 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5461 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5521 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5581 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5641 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5761 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
     5821 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     5881 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     5941 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     6001 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     6061 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     6121 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     6181 catcg
//
