LOCUS       sequence-268556-       10319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            C-terminal V5-TurboID for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10319)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-hcRed-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..10319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3390..4073
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(4075..4099)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4101..4142
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4149..5105
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(5111..5131)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    5160..5748
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5213..5233)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5631..5642)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5762..6261
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5797..5820)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6194..6213
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6282..6881
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6282..6301)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6778..6798
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7019..7252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7324..7445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7361..7380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7415..7434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7485..7620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7640..7659)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7641..7659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7669..7686)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7669..7685)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7678..7700)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7827..8282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7914..7933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8124..8145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8308..8412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8413..8481,8482..9273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9444..10032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9933..9952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10186..10203
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gttggcacca tggtgagcgg cctgctgaag gagagtatgc
     3421 gcatcaagat gtacatggag ggcaccgtga acggccacta cttcaagtgc gagggcgagg
     3481 gcgacggcaa ccccttcgcc ggcacccaga gcatgagaat ccacgtgacc gagggcgccc
     3541 ccctgccctt cgccttcgac atcctggccc cctgctgcga gtacggcagc aggaccttcg
     3601 tgcaccacac cgccgagatc cccgacttct tcaagcagag cttccccgag ggcttcacct
     3661 gggagagaac caccacctac gaggacggcg gcatcctgac cgcccaccag gacaccagcc
     3721 tggagggcaa ctgcctgatc tacaaggtga aggtgcacgg caccaacttc cccgccgacg
     3781 gccccgtgat gaagaacaag agcggcggct gggagcccag caccgaggtg gtgtaccccg
     3841 agaacggcgt gctgtgcggc cggaacgtga tggccctgaa ggtgggcgac cggcacctga
     3901 tctgccacca ctacaccagc taccggagca agaaggccgt gcgcgccctg accatgcccg
     3961 gcttccactt caccgacatc cggctccaga tgctgcggaa gaagaaggac gagtacttcg
     4021 agctgtacga ggccagcgtg gcccggtaca gcgacctgcc cgagaaggcc aacttgccaa
     4081 ctttcttgta caaagtgggc ggcaagccca tccccaaccc cctgctgggc ctggacagca
     4141 ccgctagcaa agacaatact gtgcctctga agctgatcgc tctcctggct aatggcgagt
     4201 tccatagtgg cgaacagctg ggagaaaccc tgggcatgtc cagggccgct atcaacaagc
     4261 acattcagac tctgcgcgac tggggcgtgg acgtgttcac cgtgcccgga aagggctact
     4321 ctctgcccga gcctatcccg ctgctgaacg ctaaacagat tctgggacag ctggacggcg
     4381 ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg gatcgaatcg
     4441 gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg agaggaagca
     4501 gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg ttttggcgcc
     4561 tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc atggcagaag
     4621 cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc
     4681 aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc gatgctgcac
     4741 agatcgtcat tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca
     4801 atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat actctggccg
     4861 ctacgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa ggcctggctc
     4921 catatctgcc acggtgggag aagctggata acttcatcaa tagacccgtg aagctgatca
     4981 ttggggacaa agagattttc gggattagcc gggggattga taaacaggga gccctgctgc
     5041 tggaacagga cggagttatc aaaccctgga tgggcggaga aatcagtctg cggtctgccg
     5101 aaaagtaagc caactttatt atacatagtt gatatccagc acagtggcgg ccgctcgaca
     5161 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5221 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5281 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5341 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5401 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5461 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5521 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5581 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5641 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5701 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac
     5761 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     5821 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     5881 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     5941 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     6001 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     6061 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     6121 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     6181 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     6241 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     6301 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     6361 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     6421 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6481 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6541 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6601 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6661 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6721 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6781 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6841 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     6901 agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga
     6961 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7021 gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7081 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7141 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7201 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7261 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7321 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7381 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7441 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     7501 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7561 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7621 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     7681 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     7741 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7801 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     7861 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7921 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7981 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8041 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8101 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8161 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8221 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     8281 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8341 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8401 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8461 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8521 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8581 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8641 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8701 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8761 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8821 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8881 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8941 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9001 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9061 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9121 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9181 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9241 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9301 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9361 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9421 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9481 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9541 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9601 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9661 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9721 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9781 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9841 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9901 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9961 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10021 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10081 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    10141 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10201 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10261 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaat
//
