LOCUS       sequence-268559-       11759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11759)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TLK2-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..11759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3965..3976)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5701..5725)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5727..5768
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5775..6545
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(6551..6571)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6600..7188
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6653..6673)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7071..7082)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7202..7701
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7237..7260)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7634..7653
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7722..8321
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7722..7741)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8218..8238
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8459..8692
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8764..8885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8801..8820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8855..8874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8925..9060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9080..9099)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9081..9099)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9109..9126)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9109..9125)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9118..9140)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9267..9722
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9354..9373)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9564..9585
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9748..9852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9853..9921,9922..10713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10884..11472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11373..11392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11626..11643
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gttggcatgg aagaattgca tagcctggac ccacgacggc
     3421 aggaattatt ggaggccagg tttactggag taggtgttag taagggacca cttaatagtg
     3481 agtcttccaa ccagagcttg tgcagcgtcg gatccttgag tgataaagaa gtagagactc
     3541 ccgagaaaaa gcagaatgac cagcgaaatc ggaaaagaaa agctgaacca tatgaaacta
     3601 gccaagggaa aggcactcct aggggacata aaattagtga ttactttgag tttgctgggg
     3661 gaagcgcgcc aggaaccagc cctggcagaa gtgttccacc agttgcacga tcctcaccgc
     3721 aacattcctt atccaatccc ttaccgcgac gagtagaaca gcccctctat ggtttagatg
     3781 gcagtgctgc aaaggaggca acggaggagc agtctgctct gccaaccctc atgtcagtga
     3841 tgctagcaaa acctcggctt gacacagagc agctggcgca aaggggagct ggcctctgct
     3901 tcacttttgt ttcagctcag caaaacagtc cctcatctac gggatctggc aacacagagc
     3961 attcctgcag ctcccaaaaa cagatctcca tccagcacag acagacccag tccgacctca
     4021 caatagaaaa aatatctgca ctagaaaaca gtaagaattc tgacttagag aagaaggagg
     4081 gaagaataga tgatttatta agagccaact gtgatttgag acggcagatt gatgaacagc
     4141 aaaagatgct agagaaatac aaggaacgat taaatagatg tgtgacaatg agcaagaaac
     4201 tccttataga aaagtcaaaa caagagaaga tggcgtgtag agataagagc atgcaagacc
     4261 gcttgagact gggccacttt actactgtcc gacacggagc ctcatttact gaacagtgga
     4321 cagatggtta tgcttttcag aatcttatca agcaacagga aaggataaat tcacagaggg
     4381 aagagataga aagacaacgg aaaatgttag caaagcggaa acctcctgcc atgggtcagg
     4441 cccctcctgc aaccaatgag cagaaacagc ggaaaagcaa gaccaatgga gctgaaaatg
     4501 aaacgccctc ttctgggaat acagagctaa aggatacagc cccagcctta ggagcccaca
     4561 gtttacttag gttaacgtta gcagaatacc atgaacaaga agaaatcttc aaactcagat
     4621 taggtcatct taaaaaggag gaagcagaga tccaggcaga gctggagaga ctagaaaggg
     4681 ttagaaatct acatatcagg gaactaaaaa ggatacataa tgaagataat tcacaattta
     4741 aagatcatcc aacgctaaat gacagatatt tgttgttaca tcttttgggt agaggaggtt
     4801 tcagtgaagt ttacaaggca tttgatctaa cagagcaaag atacgtagct gtgaaaattc
     4861 accagttaaa taaaaactgg agagatgaga aaaaggagaa ttaccacaag catgcatgta
     4921 gggaataccg gattcataaa gagctggatc atcccagaat agttaagctg tatgattact
     4981 tttcactgga tactgactcg ttttgtacag tattagaata ctgtgaggga aatgatctgg
     5041 acttctacct gaaacagcac aaattaatgt cggagaaaga ggcccggtcc attatcatgc
     5101 agattgtgaa tgctttaaag tacttaaatg aaataaaacc tcccatcata cactatgacc
     5161 tcaaaccagg taatattctt ttagtaaatg gtacagcgtg tggagagata aaaattacag
     5221 attttggtct ttcgaagatc atggatgatg atagctacaa ttcagtggat ggcatggagc
     5281 taacatcaca aggtgctggt acttattggt atttaccacc agagtgtttt gtggttggga
     5341 aagaaccacc aaagatctca aataaagttg atgtgtggtc ggtgggtgtg atcttctatc
     5401 agtgtcttta tggaaggaag ccttttggcc ataaccagtc tcagcaagac atcctacaag
     5461 agaatacgat tcttaaagct actgaagtgc agttcccgcc aaagccagta gtaacacctg
     5521 aagcaaaggc gtttattcga cgatgcttgg cctaccgaaa ggaggaccgc attgatgtcc
     5581 agcagctggc ctgtgatccc tacttgttgc ctcacatccg aaagtcagtc tctacaagta
     5641 gccctgctgg agctgctatt gcatcaacct ctggggcgtc caataacagt tcttctaatt
     5701 gcccaacttt cttgtacaaa gtgggcggca agcccatccc caaccccctg ctgggcctgg
     5761 acagcaccgc tagcatcccg ctgctgaacg ctaaacagat tctgggacag ctggacggcg
     5821 ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg gatcgaatcg
     5881 gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg agaggaagca
     5941 gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg ttttggcgcc
     6001 tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc atggcagaag
     6061 cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc
     6121 aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc gatgctgcac
     6181 agatcgtcat tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca
     6241 atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat actctggccg
     6301 ctatgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa ggcctggctc
     6361 catatctgtc acggtgggag aagctggata acttcatcaa tagacccgtg aagctgatca
     6421 ttggggacaa agagattttc gggattagcc gggggattga taaacaggga gccctgctgc
     6481 tggaacagga cggagttatc aaaccctgga tgggcggaga aatcagtctg cggtctgccg
     6541 aaaagtaagc caactttatt atacatagtt gatatccagc acagtggcgg ccgctcgaca
     6601 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6661 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6721 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6781 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6841 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6901 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6961 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     7021 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     7081 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7141 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac
     7201 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     7261 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     7321 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     7381 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     7441 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     7501 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     7561 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     7621 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     7681 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     7741 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     7801 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     7861 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     7921 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     7981 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     8041 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     8101 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     8161 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     8221 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     8281 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     8341 agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga
     8401 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8461 gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8521 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8581 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8641 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8701 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8761 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8821 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8881 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     8941 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     9001 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9061 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     9121 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     9181 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     9241 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     9301 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9361 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9421 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     9481 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9541 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9601 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9661 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     9721 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9781 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9841 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9901 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9961 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    10021 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10081 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    10141 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10201 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10261 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10321 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10381 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10441 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10501 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10561 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    10621 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10681 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    10741 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    10801 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10861 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10921 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10981 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    11041 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11101 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11161 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11221 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11281 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11341 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11401 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    11461 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11521 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11581 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    11641 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    11701 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaat
//
