LOCUS       sequence-268566-       10889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with C-terminal V5
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10889)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK2-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..10889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5422..5446)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5445..5486
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5545..5564)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    complement(5681..5701)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    5730..6318
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5783..5803)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6201..6212)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6332..6831
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6367..6390)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6764..6783
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6852..7451
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6852..6871)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7348..7368
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7589..7822
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7894..8015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7931..7950)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7985..8004
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8055..8190
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8210..8229)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8211..8229)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8239..8256)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8239..8255)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8248..8270)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8397..8852
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8484..8503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8694..8715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8878..8982
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8983..9051,9052..9843)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9201..9220)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10014..10602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10503..10522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10756..10773
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gcagaagggc cgtcaaggcc caccatgaca tcgacgggga
     3421 aggacggcgg cgcgcagcac gcgcagtatg ttgggcccta ccggctggag aagacgctgg
     3481 gcaaggggca gacaggtctg gtgaagctgg gggttcactg cgtcacctgc cagaaggtgg
     3541 ccatcaagat cgtcaaccgt gagaagctca gcgagtcggt gctgatgaag gtggagcggg
     3601 agatcgcgat cctgaagctc attgagcacc cccacgtcct aaagctgcac gacgtttatg
     3661 aaaacaaaaa atatttgtac ctggtgctag aacacgtgtc aggtggtgag ctcttcgact
     3721 acctggtgaa gaaggggagg ctgacgccta aggaggctcg gaagttcttc cggcagatca
     3781 tctctgcgct ggacttctgc cacagccact ccatatgcca cagggatctg aaacctgaaa
     3841 acctcctgct ggacgagaag aacaacatcc gcatcgcaga ctttggcatg gcgtccctgc
     3901 aggttggcga cagcctgttg gagaccagct gtgggtcccc ccactacgcc tgccccgagg
     3961 tgatccgggg ggagaagtat gacggccgga aggcggacgt gtggagctgc ggcgtcatcc
     4021 tgttcgcctt gctggtgggg gctctgccct tcgacgatga caacttgcga cagctgctgg
     4081 agaaggtgaa gcggggcgtg ttccacatgc cgcactttat cccgcccgac tgccagagtc
     4141 tgctacgggg catgatcgag gtggacgccg cacgccgcct cacgctagag cacattcaga
     4201 aacacatatg gtatataggg ggcaagaatg agcccgaacc agagcagccc attcctcgca
     4261 aggtgcagat ccgctcgctg cccagcctgg aggacatcga ccccgacgtg ctggacagca
     4321 tgcactcact gggctgcttc cgagaccgca acaagctgct gcaggacctg ctgtccgagg
     4381 aggagaacca ggagaagatg atttacttcc tcctcctgga ccggaaagaa aggtacccga
     4441 gccaggagga tgaggacctg cccccccgga acgagataga ccctccccgg aagcgtgtgg
     4501 actccccgat gctgaaccgg cacggcaagc ggcggccaga acgcaaatcc atggaggtgc
     4561 tcagcgtgac ggacggcggc tccccggtgc ctgcgcggcg ggccattgag atggcccagc
     4621 acggccagag gtctcggtcc atcagcggtg cctcctcagg cctttccacc agcccactca
     4681 gcagcccccg ggtgacccct cacccctcac caaggggcag tcccctcccc acccccaagg
     4741 ggacacctgt ccacacgcca aaggagagcc cggctggcac gcccaacccc acgcccccgt
     4801 ccagccccag cgtcggaggg gtgccctgga gggcgcggct caactccatc aagaacagct
     4861 ttctgggctc accccgcttc caccgccgga aactgcaagt tccgacgccg gaggagatgt
     4921 ccaacctgac accagagtcg tccccagagc tggcgaagaa gtcctggttt gggaacttca
     4981 tcagcctgga gaaggaggag cagatcttcg tggtcatcaa agacaaacct ctgagctcca
     5041 tcaaggctga catcgtgcac gccttcctgt cgattcccag tctcagccac agcgtcatct
     5101 cccaaacgag cttccgggcc gagtacaagg ccacgggggg gccagccgtg ttccagaagc
     5161 cggtcaagtt ccaggttgat atcacctaca cggagggtgg ggaggcgcag aaggagaacg
     5221 gcatctactc cgtcaccttc accctgctct caggccccag ccgtcgcttc aagagggtgg
     5281 tggagaccat ccaggcccag ctgctgagca cacacgaccc gcctgcggcc cagcacttgt
     5341 cagacaccac taactgtatg gaaatgatga cggggcggct ttccaaatgt ggaattatcc
     5401 cgaaaagttc aggcctcatg ggcccagctt tcttgtacaa agtgggcaag cctatcccta
     5461 accctctcct cggtctcgat tctacgtaat aacactggat ataccaccgt tgatatatcc
     5521 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     5581 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     5641 ttttatccgg cctttattca cattcttgcc cgcctgtaat caactttatt atacatagtt
     5701 gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa
     5761 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5821 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5881 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5941 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6001 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6061 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6121 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6181 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6241 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6301 tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag
     6361 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     6421 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     6481 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     6541 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     6601 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     6661 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     6721 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     6781 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     6841 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6901 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6961 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     7021 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     7081 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     7141 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     7201 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     7261 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     7321 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     7381 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     7441 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     7501 atgcatctcg agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7561 agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa
     7621 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7681 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7741 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7801 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7861 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7921 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7981 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8041 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8101 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8161 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     8221 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8281 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8341 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8401 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8461 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8521 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8581 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8641 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8701 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8761 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8821 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8881 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8941 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     9001 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     9061 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9121 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9181 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     9241 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9301 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9361 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9421 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9481 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9541 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9601 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9661 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9721 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9781 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9841 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9901 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9961 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    10021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10141 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10381 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10621 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10681 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    10741 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    10801 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
    10861 tggaaagcgg gcagtgagcg caacgcaat
//
