LOCUS       sequence-268571-        8582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for shRNA-mediated knockdown, with co-expression 
            with tdTomato-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8582)
  TITLE     Madison Lab plasmids
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BII-Sh-BnTW
FEATURES             Location/Qualifiers
     source          1..8582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(308..324)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..324)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        580..828
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     580..600
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     751..770
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     regulatory      1557..1566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1563..1958
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1977..2030
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2037..2063
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2037..2063
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2070..3497
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             3501..3521
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3558..4146
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3611..3631)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4029..4040)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4226..4360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4250..4269)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4304..4323
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5139..5201
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5331..5348)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5331..5347)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5340..5362)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5489..5944
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5576..5595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5786..5807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5970..6074
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6075..6143,6144..6935)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6293..6312)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7106..7694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7595..7614
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7848..7865
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7982..8003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8018..8023,8024..8041,8042..8048)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8056..8072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8061..8083
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8080..8096
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8080..8096
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ccactagttc tagagggaca gccccccccc aaagccccca gggatgtaat
      361 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
      421 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
      481 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
      541 ggatacgggg aaaaggcctc cagggccgcc cccttcaccg agggcctatt tcccatgatt
      601 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
      661 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      721 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
      781 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg gagacgctcg
      841 acccgtctcg tttttgcctc caaggcctgc aggagtcaat gggaaaaacc cattggagcc
      901 aagtacactg actcaatagg gactttccat tgggttttgc ccagtacata aggtcaatag
      961 ggggtgagtc aacaggaaag tcccattgga gccaagtaca ttgagtcaat agggactttc
     1021 caatgggttt tgcccagtac ataaggtcaa tgggaggtaa gccaatgggt ttttcccatt
     1081 actgacatgt atactgagtc attagggact ttccaatggg ttttgcccag tacataaggt
     1141 caataggggt gaatcaacag gaaagtccca ttggagccaa gtacactgag tcaataggga
     1201 ctttccattg ggttttgccc agtacaaaag gtcaataggg ggtgagtcaa tgggtttttc
     1261 ccattattgg cacatacata aggtcaatag gggtgactag tggagaagag catgcttgag
     1321 ggctgagtgc ccctcagtgg gcagagagca catggcccac agtccctgag aagttggggg
     1381 gaggggtggg caattgaact ggtgcctaga gaaggtgggg cttgggtaaa ctgggaaagt
     1441 gatgtggtgt actggctcca cctttttccc cagggtgggg gagaaccata tataagtgca
     1501 gtagtctctg tgaacattcg gtttagtgaa cctgcaggtc ggccgccacg accggtgcca
     1561 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     1621 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     1681 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     1741 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     1801 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     1861 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     1921 ttcgtgaatt gctgccctct ggttatgtgt gggagggcgc tagccttggc tccggcgagg
     1981 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca ctcggctacc
     2041 cctacgacgt gcccgactac gccagcggca tggtgagcaa gggcgaggag gtcatcaaag
     2101 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
     2161 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     2221 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
     2281 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
     2341 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
     2401 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
     2461 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     2521 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
     2581 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
     2641 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
     2701 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggcaccg
     2761 gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca
     2821 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
     2881 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     2941 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
     3001 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
     3061 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
     3121 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
     3181 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
     3241 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
     3301 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
     3361 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
     3421 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca
     3481 tggacgagct gtacaagggc cccaagaaga agcgcaaggt gggcgcctaa gtacaagtaa
     3541 gagttcgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3601 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3661 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3721 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3781 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3841 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3901 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3961 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4021 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4081 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4141 ccccgcctgg aattaattct ggctaagcac ttcgtggccg tcgatcgttt aaagggaggt
     4201 agtgagtcga gatatcgata ctagtgatcc agacatgata agatacattg atgagtttgg
     4261 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4321 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4381 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     4441 caaatgtggt atggctgatt atgatcctct agagggacag ccccccccca aagcccccag
     4501 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     4561 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     4621 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     4681 cacctggggg gatacgggga aaaagccata ccaatggagc tcacggggac agcccccccc
     4741 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     4801 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     4861 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     4921 tgagcctgca gacacctggg gggatacggg gaaaaagaat tcgataaaag ttttgttact
     4981 ttatagaaga aattttgagt ttttgttttt tttaataaat aaataaacat aaataaattg
     5041 tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa
     5101 attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacacatga
     5161 ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaatctag ctgcgtgttc
     5221 tgcagcgtgt cgagcatctt catctgctcc atcacgctgt aaaacacatt tgcaccgcga
     5281 gtctgcccgt cctccacggg ttcaaaaacg tgaatgaacg aggcgcgctc actggccgtc
     5341 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     5401 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5461 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     5521 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5581 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5641 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5701 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5761 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5821 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5881 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5941 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6001 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6061 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6121 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6181 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6241 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6301 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6361 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6421 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6481 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6541 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6601 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6661 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6721 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6781 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6841 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6901 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6961 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7021 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7741 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7801 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7861 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7921 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7981 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8041 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     8101 attacgccaa gcgcgcccgc cgggtaactc acggggtatc catgtccatt tctgcggcat
     8161 ccagccagga tacccgtcct cgctgacgta atatcccagc gccgcaccgc tgtcattaat
     8221 ctgcacaccg gcacggcagt tccggctgtc gccggtattg ttcgggttgc tgatgcgctt
     8281 cgggctgacc atccggaact gtgtccggaa aagccgcgac gaactggtat cccaggtggc
     8341 ctgaacgaac agttcaccgt taaaggcgtg catggccaca ccttcccgaa tcatcatggt
     8401 aaacgtgcgt tttcgctcaa cgtcaatgca gcagcagtca tcctcggcaa actctttcca
     8461 tgccgcttca acctcgcggg aaaaggcacg ggcttcttcc tccccgatgc ccagatagcg
     8521 ccagcttggg cgatgactga gccggaaaaa agacccgacg atatgatcct gatgcagcta
     8581 ga
//
