LOCUS       sequence-268576-        7921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CEN vector with Hygromycin resistance marker for triple Gateway 
            insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7921)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p41Hyg 3-F GW
FEATURES             Location/Qualifiers
     source          1..7921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..504
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     545..563
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(601..623)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     723..742
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     gene            817..2392
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        817..1160
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1161..2189
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1161..2189
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      2195..2392
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      complement(2522..2977)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2659..2680)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2871..2890
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3103..3125
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3118..3134
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3144..3163
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3144..3162
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(3181..3428)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     3394..3412
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     3430..3446
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3431..3447
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3495..3618
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3764..3866
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3867..4550
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(3933..3952)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4870..5175
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5054..5073
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5523..5646)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5667..5683)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5667..5683)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5720..5740)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5720..5738)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5759..5775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5759..5775)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5772..5794)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5783..5799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5807..5813,5814..5831,5832..5837))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5852..5873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5990..6007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6161..6749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6241..6260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6920..7711,7712..7780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7543..7562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7781..7885)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
       61 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
      121 actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat
      181 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
      241 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
      301 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
      361 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
      421 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
      481 cgtgtaagtt acaggcaagc gatccgtcta agaaaccatt attatcatga cattaaccta
      541 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
      601 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
      661 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      721 tgcggcatca gagcagattg tactgagagt gcaccatacc acagcttttc aagatctgtt
      781 tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc
      841 cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag
      901 cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga
      961 agcaaaaatt acggctcctc gctgcggacc tgcgagcagg gaaacgctcc cctcacagac
     1021 gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca
     1081 ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc
     1141 acatccgaac ataaacaacc atgggtaaaa agcctgaact caccgcgacg tctgtcgaga
     1201 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     1261 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     1321 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     1381 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     1441 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     1501 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     1561 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     1621 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     1681 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     1741 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     1801 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     1861 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     1921 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     1981 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     2041 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     2101 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     2161 ccagcactcg tccgagggca aaggaataat cagtactgac aataaaaaga ttcttgtttt
     2221 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     2281 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     2341 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
     2401 tcgatactaa cgccgccatc cagtgtcgaa aacgagctcg aattcatcga tgatatcaga
     2461 tccactagct atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2521 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2581 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2641 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     2701 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     2761 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     2821 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     2881 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     2941 acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca
     3001 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     3061 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     3121 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccggcc
     3181 gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc aaggttttca gtataatgtt
     3241 acatgcgtac acgcgtctgt acagaaaaaa aagaaaaatt tgaaatataa ataacgttct
     3301 taatactaac ataactataa aaaaataaat agggacctag acttcaggtt gtctaactcc
     3361 ttccttttcg gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg acataactaa
     3421 ttacatgact cgaggtcgac ggtatcgata agcttgatat cgaattcctg cagccccgac
     3481 ggccagtgaa ttatcaacta tgtataataa agttgaacga gaaacgtaaa atgatataaa
     3541 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     3601 acatatccag tcactatggc ggccgtaagt tggcagcatc acccgacgca ctttgcgccg
     3661 aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg
     3721 ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag
     3781 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
     3841 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     3901 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     3961 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     4021 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     4081 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     4141 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     4201 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     4261 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     4321 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     4381 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     4441 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     4501 gcgatgagtg gcaggggggg cgtaaacgcc gcgtggatcc ggcttactaa aagccagata
     4561 acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac
     4621 ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc
     4681 gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa
     4741 ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag
     4801 ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg
     4861 gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
     4921 gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc
     4981 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
     5041 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
     5101 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
     5161 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgatacagt
     5221 agaaattaca gaaactttat cacgtttagt aagtatagag gctgaaaatc cagatgaagc
     5281 cgaacgactt gtaagagaaa agtataagag ttgtgaaatt gttcttgatg cagatgattt
     5341 tcaggactat gacactagcg tatatgaata ggtagatgtt tttattttgt cacacaaaaa
     5401 agaggctcgc acctcttttt cttatttctt tttatgattt aatacggcat tgaggacaat
     5461 agcgagtagg ctggatacga cgattccgtt tgagaagaac atttggaagg ctgtcggtcg
     5521 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     5581 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttctataca
     5641 aagttgatag cttggcgtaa tcgggggatc cactagttct agagcggccg ccaccgcggt
     5701 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     5761 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5821 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5881 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5941 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6061 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcctttt
     7921 c
//
