LOCUS       sequence-268583-        9716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-host plasmid expressing gfp under pLtetO promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Lee HH, Ostrov N, Wong BG, Gold MA, Khalil AS, Church GM
  TITLE     Functional genomics of the rapidly replicating bacterium Vibrio 
            natriegens by CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Apr 8. pii: 10.1038/s41564-019-0423-8. doi: 
            10.1038/s41564-019-0423-8.
  PUBMED    30962569
REFERENCE   2  (bases 1 to 9716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Apr 8. pii: 10.1038/s41564-019-0423-8. doi: 
            10.1038/s41564-019-0423-8."
COMMENT     Sequence Label: pRSF-pLtetO-gfp
FEATURES             Location/Qualifiers
     source          1..9716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..19,20..25,26..42,43..48,49..74)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             84..95
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             102..815
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(132..160)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     755..775
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      878..924
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1607..2001
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            2343..2429
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             3668..4639
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEETGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             5150..5989
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             5976..6827
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     promoter        join(7093..7098,7099..7116,7117..7123)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    7131..7147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7136..7158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     RBS             7172..7194
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7202..8284
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7221..7240)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(8418..8486,8487..9278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      9477..9562
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9654..9681
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc
       61 aggacacact gaccgaattc attaaagagg agaaaggtac catgagtaaa ggagaagaac
      121 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      181 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      241 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
      301 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
      361 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
      421 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
      481 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
      541 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
      601 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
      661 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
      721 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
      781 ctgggattac acatggcatg gatgaactat acaaataaaa gcttgatatc gaattcctgc
      841 agcccggggg atcccatggt acgcgtgcta gaggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatgcggcc gcgatcaagc aggtgcgaca gacgtcatac
      961 tagatatcaa gcgacttctc ctatcccctg ggaacacatc aatctcaccg gagaatatcg
     1021 ctggccaaag ccttagcgta ggattccgcc ccttcccgca aacgacccca aacaggaaac
     1081 gcagctgaaa cgggaagctc aacacccact gacgcatggg ttgttcaggc agtacttcat
     1141 caaccagcaa ggcggcactt tcggccatcc gccgcgcccc acagctcggg cagaaaccgc
     1201 gacgcttaca gctgaaagcg accaggtgct cggcgtggca agactcgcag cgaacccgta
     1261 gaaagccatg ctccagccgc ccgcattgga gaaattcttc aaattcccgt tgcacatagc
     1321 ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa
     1381 cgatctgata gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat
     1441 cccggctggc cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt
     1501 tacatacagt ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt
     1561 gctagacatt gccagccagt gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat
     1621 aactgtcacg cccccctgca ataactgtca cgaacccctg caataactgt cacgccccca
     1681 aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt
     1741 atctaagaat aatccactag gcgcggttat cagcgccctt gtggggcgct gctgcccttg
     1801 cccaatatgc ccggccagag gccggatagc tggtctattc gctgcgctag gctacacacc
     1861 gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac
     1921 cccgcagaaa tacgctggag cgcttttagc cgctttagcg gcctttcccc ctacccgaag
     1981 ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca gcccggctca
     2041 tccttctggc gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca
     2101 tccattgccg ccatgagccg atcctccggc cactcgctgc tgttcacctt ggccaaaatc
     2161 atggccccca ccagcaccct gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt
     2221 gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag cttggccagc
     2281 cgatccgccg ccttgttgct ccccttaacc atcttgacac cccattgtta atgtgctgtc
     2341 tcgtaggcta tcatggaggc acagcggcgg caatcccgac cctactttgt aggggagggg
     2401 cacttaccgg tttctcttcg agaaactggc ctaacggcca cccttcgggc ggtgcgctct
     2461 ccgagggcca ttgcatggag ccgaaaagca aaagcaacag cgaggcagca tggcgattta
     2521 tcaccttacg gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg ccaaggccga
     2581 ctacatccag cgcgaaggca agtatgcccg cgacatggat gaagtcttgc acgccgaatc
     2641 cgggcacatg ccggagttcg tcgagcggcc cgccgactac tgggatgctg ccgacctgta
     2701 tgaacgcgcc aatgggcggc tgttcaagga ggtcgaattt gccctgccgg tcgagctgac
     2761 cctcgaccag cagaaggcgc tggcgtccga gttcgcccag cacctgaccg gtgccgagcg
     2821 cctgccgtat acgctggcca tccatgccgg tggcggcgag aacccgcact gccacctgat
     2881 gatctccgag cggatcaatg acggcatcga gcggcccgcc gctcagtggt tcaagcggta
     2941 caacggcaag accccggaga agggcggggc acagaagacc gaagcgctca agcccaaggc
     3001 atggcttgag cagacccgcg aggcatgggc cgaccatgcc aaccgggcat tagagcgggc
     3061 tggccacgac gcccgcattg accacagaac acttgaggcg cagggcatcg agcgcctgcc
     3121 cggtgttcac ctggggccga acgtggtgga gatggaaggc cggggcatcc gcaccgaccg
     3181 ggcagacgtg gccctgaaca tcgacaccgc caacacccag atcatcgact tacaggaata
     3241 ccgggaggca atagaccatg aacgcaatcg acagagtgaa gaaatccaga ggcatcaacg
     3301 agttagcgga gcagatcgaa ccgctggccc agagcatggc gacactggcc gacgaagccc
     3361 ggcaggtcat gagccagacc cagcaggcca gcgaggcgca ggcggcggag tggctgaaag
     3421 cccagcgcca gacaggggcg gcatgggtgg agctggccaa agagttgcgg gaggtagccg
     3481 ccgaggtgag cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg cactggaagc
     3541 tatggctaac cgtgatgctg gcttccatga tgcctacggt ggtgctgctg atcgcatcgt
     3601 tgctcttgct cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg ctgcgcttgg
     3661 tggcccgatg aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg
     3721 ctgtgagcgc ttcgatatcg gcgtcaggga cgccaccacc ggccagatga tgaaccggga
     3781 atggtcagcc gccgaagtgc tccagaacac gccatggctc aagcggatga atgcccaggg
     3841 caatgacgtg tatatcaggc ccgccgagca ggagcggcat ggtctggtgc tggtggacga
     3901 cctcagcgag tttgacctgg atgacatgaa agccgagggc cgggagcctg ccctggtagt
     3961 ggaaaccagc ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg caggcggtga
     4021 acttcggggg cagattgccc ggacgctggc cagcgagtac gacgccgacc cggccagcgc
     4081 cgacagccgc cactatggcc gcttggcggg cttcaccaac cgcaaggaca agcacaccac
     4141 ccgcgccggt tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc
     4201 tggcccggcg ctggtgcagc aggctggcca gcagatcgag caggcccagc ggcagcagga
     4261 gaaggcccgc aggctggcca gcctcgaact gcccgagcgg cagcttagcc gccaccggcg
     4321 cacggcgctg gacgagtacc gcagcgagat ggccgggctg gtcaagcgct tcggtgatga
     4381 cctcagcaag tgcgacttta tcgccgcgca gaagctggcc agccggggcc gcagtgccga
     4441 ggaaaccggc aaggccatgg ccgaggccag cccagcgctg gcagagcgca agcccggcca
     4501 cgaagcggat tacatcgagc gcaccgtcag caaggtcatg ggtctgccca gcgtccagct
     4561 tgcgcgggcc gagctggcac gggcaccggc accccgccag cgaggcatgg acaggggcgg
     4621 gccagatttc agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc
     4681 acgcacagaa gggggtttta tggaatacga aaaaagcgct tcagggtcgg tctacctgat
     4741 caaaagtgac aagggctatt ggttgcccgg tggctttggt tatacgtcaa acaaggccga
     4801 ggctggccgc ttttcagtcg ctgatatggc cagccttaac cttgacggct gcaccttgtc
     4861 cttgttccgc gaagacaagc ctttcggccc cggcaagttt ctcggtgact gatatgaaag
     4921 accaaaagga caagcagacc ggcgacctgc tggccagccc tgacgctgta cgccaagcgc
     4981 gatatgccga gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg
     5041 acgaatacga ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag ggcgggggta
     5101 gtgaccccgc cagcgcctaa ccaccaactg cctgcaaagg aggcaatcaa tggctaccca
     5161 taagcctatc aatattctgg aggcgttcgc agcagcgccg ccaccgctgg actacgtttt
     5221 gcccaacatg gtggccggta cggtcggggc gctggtgtcg cccggtggtg ccggtaaatc
     5281 catgctggcc ctgcaactgg ccgcacagat tgcaggcggg ccggatctgc tggaggtggg
     5341 cgaactgccc accggcccgg tgatctacct gcccgccgaa gacccgccca ccgccattca
     5401 tcaccgcctg cacgcccttg gggcgcacct cagcgccgag gaacggcaag ccgtggctga
     5461 cggcctgctg atccagccgc tgatcggcag cctgcccaac atcatggccc cggagtggtt
     5521 cgacggcctc aagcgcgccg ccgagggccg ccgcctgatg gtgctggaca cgctgcgccg
     5581 gttccacatc gaggaagaaa acgccagcgg ccccatggcc caggtcatcg gtcgcatgga
     5641 ggccatcgcc gccgataccg ggtgctctat cgtgttcctg caccatgcca gcaagggcgc
     5701 ggccatgatg ggcgcaggcg accagcagca ggccagccgg ggcagctcgg tactggtcga
     5761 taacatccgc tggcagtcct acctgtcgag catgaccagc gccgaggccg aggaatgggg
     5821 tgtggacgac gaccagcgcc ggttcttcgt ccgcttcggt gtgagcaagg ccaactatgg
     5881 cgcaccgttc gctgatcggt ggttcaggcg gcatgacggc ggggtgctca agcccgccgt
     5941 gctggagagg cagcgcaaga gcaagggggt gccccgtggt gaagcctaag aacaagcaca
     6001 gcctcagcca cgtccggcac gacccggcgc actgtctggc ccccggcctg ttccgtgccc
     6061 tcaagcgggg cgagcgcaag cgcagcaagc tggacgtgac gtatgactac ggtgacggca
     6121 agcggatcga gttcagcggc ccggagccgc tgggcgctga tgatctgcgc atcctgcaag
     6181 ggctggtggc catggctggg cctaatggcc tagtgcttgg cccggaaccc aagaccgaag
     6241 gcggacggca gctccggctg ttcctggaac ccaagtggga ggccgtcacc gctgatgcca
     6301 tggtggtcaa aggtagctat cgggcgctgg caaaggaaat cggggcagag gtcgatagtg
     6361 gtggggcgct caagcacata caggactgca tcgagcgcct ttggaaggta tccatcatcg
     6421 cccagaatgg ccgcaagcgg caggggtttc ggctgctgtc ggagtacgcc agcgacgagg
     6481 cggacgggcg cctgtacgtg gccctgaacc ccttgatcgc gcaggccgtc atgggtggcg
     6541 gccagcatgt gcgcatcagc atggacgagg tgcgggcgct ggacagcgaa accgcccgcc
     6601 tgctgcacca gcggctgtgt ggctggatcg accccggcaa aaccggcaag gcttccatag
     6661 ataccttgtg cggctatgtc tggccgtcag aggccagtgg ttcgaccatg cgcaagcgcc
     6721 gccagcgggt gcgcgaggcg ttgccggagc tggtcgcgct gggctggacg gtaaccgagt
     6781 tcgcggcggg caagtacgac atcacccggc ccaaggcggc aggctgaccc cccccactct
     6841 attgtaaaca agacattttt atcttttata ttcaatggct tattttcctg ctaattggta
     6901 ataccatgaa aaataccatg ctcagaaaag gcttaacaat attttgaaaa attgcctact
     6961 gagcgctgcc gcacagctcc ataggccgct ttcctggctt tgcttccaga tgtatgctct
     7021 tctgctccga tctgcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta
     7081 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggaa ttgtgagcgg
     7141 ataacaattt cacacaggat ctagaaataa ttttgtttaa ctttaagaag gagatataca
     7201 tatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
     7261 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     7321 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
     7381 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
     7441 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
     7501 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
     7561 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
     7621 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
     7681 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
     7741 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     7801 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     7861 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
     7921 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     7981 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
     8041 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     8101 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     8161 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     8221 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8281 gtgagcggat ctcgaagctg atccgcggcc gccacgttgt gtctcaaaat ctctaatgtt
     8341 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     8401 gtaatacaag gggtgttatg agcattcagc attttcgtgt ggcgctgatt ccgttttttg
     8461 cggcgttttg cctgccggtg tttgcgcatc cggaaaccct ggtgaaagtg aaagatgcgg
     8521 aagatcaact gggtgcgcgc gtgggctata ttgaactgga tctgaacagc ggcaaaattc
     8581 tggaatcttt tcgtccggaa gaacgttttc cgatgatgag cacctttaaa gtgctgctgt
     8641 gcggtgcggt tctgagccgt gtggatgcgg gccaggaaca actgggccgt cgtattcatt
     8701 atagccagaa cgatctggtg gaatatagcc cggtgaccga aaaacatctg accgatggca
     8761 tgaccgtgcg tgaactgtgc agcgcggcga ttaccatgag cgataacacc gcggcgaacc
     8821 tgctgctgac gaccattggc ggtccgaaag aactgaccgc gtttctgcat aacatgggcg
     8881 atcatgtgac ccgtctggat cgttgggaac cggaactgaa cgaagcgatt ccgaacgatg
     8941 aacgtgatac caccatgccg gcagcaatgg cgaccaccct gcgtaaactg ctgacgggtg
     9001 agctgctgac cctggcaagc cgccagcaac tgattgattg gatggaagcg gataaagtgg
     9061 cgggtccgct gctgcgtagc gcgctgccgg ctggctggtt tattgcggat aaaagcggtg
     9121 cgggcgaacg tggcagccgt ggcattattg cggcgctggg cccggatggt aaaccgagcc
     9181 gtattgtggt gatttatacc accggcagcc aggcgacgat ggatgaacgt aaccgtcaga
     9241 ttgcggaaat tggcgcgagc ctgattaaac attggtaatc agaattggtt aattggttga
     9301 ttaccctgtt atccctatct agagtcgacc tgcaggcatg caagcttaga tcctttgcct
     9361 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
     9421 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcattaaat
     9481 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     9541 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
     9601 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
     9661 catcctgacg gatggccttt ttgcgtttct acaaactctt tttgggtacc agatct
//
