LOCUS       sequence-268587-        5877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expression of TRE(TRE3G)-driven, tdTomato expression for 
            fluorescent labling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Abe H, Tani T, Mashiko H, Kitamura N, Hayami T, Watanabe S, Sakai K,
            Suzuki W, Mizukami H, Watakabe A, Yamamori T, Ichinohe N
  TITLE     Axonal Projections From the Middle Temporal Area in the Common 
            Marmoset.
  JOURNAL   Front Neuroanat. 2018 Oct 30;12:89. doi: 10.3389/fnana.2018.00089. 
            eCollection 2018.
  PUBMED    30425625
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neuroanat."; date: "2018-10-30"; volume: "12:89. doi"; pages: " 
            10.3389/fnana.2018.00089. eCollection 2018"
COMMENT     Sequence Label: pAAVTRE3_tdTomato
FEATURES             Location/Qualifiers
     source          1..5877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        147..525
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    263..281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    299..317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    335..353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    371..389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     434..458
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      538..547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             544..1974
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    2004..2592
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2057..2077)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2475..2486)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2624..3100
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2823..2842)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3140..3280
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3140..3269
                     /label=AAV2 ITR
     rep_origin      3355..3810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3442..3461)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3652..3673
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3827..3846)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3946..3968
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4006..4024)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4092..4196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4197..4265,4266..5057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4415..4434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5228..5816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5717..5736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttcgagt ttactcccta tcagtgatag agaacgtatg
      181 aagagtttac tccctatcag tgatagagaa cgtatgcaga ctttactccc tatcagtgat
      241 agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac cagtttactc
      301 cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag agaacgtata
      361 tccagtttac tccctatcag tgatagagaa cgtataagct ttaggcgtgt acggtgggcg
      421 cctataaaag cagagctcgt ttagtgaacc gtcagatcgc ctggagcaat tccacaacac
      481 ttttgtctta taccaacttt ccgtaccact tcctaccctc gtaaagtcga cgaattcgcc
      541 accatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg
      601 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      661 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
      721 gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac
      781 atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      841 ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg
      901 atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag
      961 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
     1021 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
     1081 accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag
     1141 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag
     1201 ggccgccacc acctgttcct ggggcatggc accggcagca ccggcagcgg cagctccggc
     1261 accgcctcct ccgaggacaa caacatggcc gtcatcaaag agttcatgcg cttcaaggtg
     1321 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1381 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     1441 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
     1501 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1561 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
     1621 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
     1681 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     1741 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag
     1801 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
     1861 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
     1921 tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa gtaaggatcc
     1981 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     2041 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2101 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2161 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2221 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2281 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     2341 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     2401 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct
     2461 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     2521 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     2581 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga
     2641 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
     2701 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
     2761 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
     2821 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
     2881 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
     2941 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
     3001 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
     3061 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac
     3121 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     3181 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     3241 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     3301 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     3361 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3421 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3481 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3541 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     3601 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3661 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     3721 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3781 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     3841 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     3901 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     3961 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4021 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4081 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4141 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4201 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4261 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4321 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4381 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4441 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4501 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4561 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4621 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4681 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4741 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4801 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4861 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4921 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4981 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5041 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5101 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5161 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5221 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5281 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5341 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5401 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5461 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5521 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5581 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5641 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5701 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5761 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5821 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
