LOCUS       sequence-26860-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Kaganovich D, Kopito R, Frydman J
  TITLE     Misfolded proteins partition between two distinct quality control 
            compartments.
  JOURNAL   Nature. 2008 Aug 28. 454(7208):1088-95.
  PUBMED    18756251
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2008 Aug 28. 454(7208):1088-95."
COMMENT     Sequence Label: pESC-LEU-GFP-VHL
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(13..15,16..18,19..729)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDXLYK"
     primer_bind     complement(58..79)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(319..338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     666..687
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acccggatct tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
       61 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      181 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      301 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      421 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      481 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      601 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
      661 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgan
      721 ctgtacaagc gcggatccgc gcaagcttgc atggaacaaa acttattttg aaaagattgg
      781 accccggatn gggaaaactg ggacgagccn agtagccgga gagcagcgt
//
