LOCUS       sequence-268602-       13394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to generate a transgene that will express an epitope tagged 
            Chitin Synthase in Drosophila using Gal4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13394)
  AUTHORS   Adler PN
  TITLE     The localization of chitin synthase mediates the patterned 
            deposition of chitin in developing Drosophila bristles
  JOURNAL   bioRxiv 2020
REFERENCE   2  (bases 1 to 13394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020"
COMMENT     Sequence Label: pUAS-kkv-OH
FEATURES             Location/Qualifiers
     source          1..13394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4875..4969
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4988..5226
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5173..5194
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             complement(5953..5964)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      7248..7257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10119..10160
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             10161..10178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          10268..10333
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10463..10483
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10755..10889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10792..10811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10846..10865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    10918..10987
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(11206..11224)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11245..11261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11245..11261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11258..11280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11269..11285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11293..11299,11300..11317,11318..11323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11338..11359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11476..11493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11647..12235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11727..11746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12406..13197,13198..13266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13029..13048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13267..13371)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccaagctt
     4861 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     4921 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
     4981 gcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca
     5041 agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag
     5101 ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca
     5161 agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga
     5221 actctgaata gggaattggg aattcgttaa cagatctgcg gccgcggctc gagatgtctg
     5281 cgatgcggca tcgcccgatg gcccctccgg gccaaggacc aggagcggga accgctgggg
     5341 agcacgtcga cagcgatgac aacaacttta ccgacgacga gagctcgccc ctcacccacg
     5401 acatttacgg cggcagccaa cgcaccatac aagagacgaa gggttgggat gttttccggg
     5461 atccgccaat caaaattgag accggatcga cggcgaacca ggaatgctta gaattaaccg
     5521 tgaagatctt gaagatcttc gcctatgtga ttacgtttat aatagtttta accggtggcg
     5581 tcatcgccaa gggcacaatg ctcttcatga cttcgcaggt gcgcaaggat aagaagatgg
     5641 agtactgcaa caaagacttg ggtcgggaca aaagcttcgt agtccgactt ccggaggagg
     5701 agcgagtggc ctggatctgg gctctgctca tagcatatgc ccttcccgag atcggagcct
     5761 tgatccgatc cgcccgcatc tgcttcttca agacctttaa ggtgccgaag acgggccact
     5821 tcctcttcgt ctggctgatg gagagcttga gtgccgtggg catggcgctg ctgatgttcg
     5881 tggttctgcc ccagatcgac gccatccagg gcgccatgct aaccaactgc ctgtgcgtcg
     5941 tgccgggcat cttcggcctt ctatcgcgca cctccaagga gggcaagcgc tttgtaaagg
     6001 tgatcatcga tctggcggcg gtggcggccc aggtgacggg cctggtgatc tggccgctgc
     6061 tggagaaccg ccgtgagctg tgggtcatcc cggtggcctg tgtcatgatc tcgtgcggct
     6121 ggtgggagaa ttacgtgtcg ccacagtccc cgctcggcct ggttcgagcc ctgggtcgca
     6181 tcaaggagga gatgaagtac actcgctact tctgccacat attcctatcc atctggaaga
     6241 tcctgctttt cttcaccgtc acactgctca tctactgggc gcaaggcgag gaacccggca
     6301 atctgtttgc catgtacgga gatgcctttg gaccccacaa gatcatagtc tacgaactgc
     6361 cagcgggact gggcggtgtg cttccagata ccctggaatc ggccaacatt gacaccgtgg
     6421 atgtggatgc cgcctacaat acggtggtgt atgtactgct tctacagatt ttcggcgcct
     6481 acttgtgcta catcttcgga aagttcgcct gcaagatcct catccaggga ttcagctacg
     6541 cgtttcccgt cagtctaact gttccgctct ctgttacgtt cctgatcgca gcctgtggaa
     6601 tccggatcga cgatccctgc ttcttccatg acaccattcc ggactatctg ttcttcacga
     6661 gcccctcgaa cttccggttc aacaactttg tcaccgagca aatggcgtgg gcctggatcc
     6721 tttggctcct cagtcagacc tggatagcac tgcacatctg gactccgaag tgcgagcgtc
     6781 tggccaccac cgagaagttg tttgtccagc ccatgtactc ctctctactg atcgatcagt
     6841 cgatggccct caaccgaagg cgcgatgacc aggcagatgt gaaaacagag gatctctcgg
     6901 agatcgaaaa ggaaaagggc gatgagtact acgagaccat ttctgtgcac acggatcgct
     6961 cctcggcacc caacaagcca tcgattaagt cctccgacaa catcacacga atctactcct
     7021 gcgcgaccat gtggcatgag acgaaggacg agatgataga gttcttgaag agcatcatgc
     7081 gaatggacga ggaccagtgt gctcgtcgcg tggctcaaaa gtatctgagg gttctcgatc
     7141 cggactatta tgaatttgag acccacatct tcttcgatga cgccttcgaa atctccgatc
     7201 acagcgacga tgatatccag tgcaatcgat tcgtcaagct cttgatagcc accatggacg
     7261 aggctgcctc cgagatccat cagaccacga tcagactgcg tccgcccaag aagtatccca
     7321 ctccgtacgg aggccgcctg gtgtggaccc ttccagggaa gaccaaattc attacgcact
     7381 taaaggacaa ggaccgcatt cgtcacagga agcgttggtc tcaggtcatg tacatgtatt
     7441 acctgctggg acatcgtcta atggagctgc ccatttcggt ggatcgcaag gatgcaattg
     7501 cggagaacac ctaccttttg accctggacg gagatattga cttcaagccg aatgcggtga
     7561 ctctactggt ggatctgatg aagaagaaca agaacctggg tgctgcctgc ggtcgcattc
     7621 atcccgtggg atcgggaccc atggtgtggt accaactttt cgaatacgcc atcggccatt
     7681 ggctgcaaaa ggccacggag cacatgattg gctgcgtgct ctgttcacct ggatgcttct
     7741 ccctgttcag gggcaaggcc ctcatggatg acaatgtgat gaagaaatac acgacacggt
     7801 cggatgaggc tcgtcactac gtgcagtacg atcagggcga ggatcgttgg ctgtgcacat
     7861 tgctcctcca gaggggatac cgcgtggagt actcggctgc cagtgatgcg tacacccact
     7921 gccccgaggg attcaatgag ttctacaacc agcggcgcag atgggtgccc tcgaccatag
     7981 ccaacatcat ggatcttctg gcggatgcga agcgcacgat caagatcaat gacaacattt
     8041 cgcttctgta catcttctac cagatgatgt tgatgggcgg caccatcctg ggacccggaa
     8101 ccattttcct tatgttggtg ggtgcattcg tggcagcctt tcgcattgac aactggacct
     8161 ccttccacta caacatcgtg ccgatcctgg ccttcatgtt tatctgcttc acctgtaagt
     8221 cgaacatcca gttgttcgtg gcccaagtcc tttcgacggc atatgccctg atcatgatgg
     8281 cggtgattgt gggtacggcc ttgcagttgg gagaggacgg aataggctcc ccttcggcca
     8341 ttttcctgat atccatggtg ggctcattct tcatagccgc atgtttgcat ccacaagagt
     8401 tctggtgcat tacatgcggc ctaatctatt tgctgtccat cccgtccatg tacctgctgc
     8461 tcatcttgta ctctatcatc aacctaaacg tcgtctcctg gggcacccgc gaggtggtgg
     8521 ctaagaagac caagaaagaa ctggaggcgg agaagaaggc cgccgaggag gccaaaaaga
     8581 gggtgaagca gaagagcatg ctgagcttcc ttcagagtgg aatcggggac aatggcgacg
     8641 aggagggctc cgtggagttc tctctcgctg gactcttccg ctgcatattc tgcacccacg
     8701 gaaaaacatc cgacgagaag cagcagctga cctccatcgc cgaatcgctg gacacgatca
     8761 aacatcgaat ggacaccatc gagagtgctg tggatcccca tggtcaccat gcctcccgac
     8821 atggcaggag gagaaccaca tccagtggct ccaaggatca ccacctgctg acctcggtgg
     8881 cggagaagtc tggcgatgaa tcggacgagt cggactcaga cacttcggcg gaaccaaagc
     8941 aggagcgaga ctttctaacc aatccctact ggatcgagga ccccgatgta cgcaagggcg
     9001 aggtcgattt tctctccagc accgagatcc agttctggaa ggatctcatc gaccagtatc
     9061 tctatcccat cgacaatgat cccgtggagc aggcccgcat agccaaagat ctgaaggagc
     9121 tgcgcgactc gtcggtgttc gcgttcttca tgatcaatgc actgttcgtg ctgatcgtgt
     9181 tcctgctgca gctgaacaag gacaacatcc acgtgaagtg gcccttcgga gtgcggacga
     9241 acatcaccta cgacgagtcc acccaggagg tgcacatctc caaggagtac cttcagctgg
     9301 agcccatcgg attggtgttc gtattcttct ttgctcttat tttgataatc cagttcacgg
     9361 ccatgctgtt ccatcgattt ggaaccattt cgcacatcct ggcctcgact gaactcaact
     9421 tctgcaagaa gaagtctgag gatctgacac aggaccaact aatagataag catgctgtgg
     9481 agatcgtcaa gaacttgcag aggctgcagg gaatcgatgg cgactacgac aatgactccg
     9541 gttccgggcc agatcgcata gccaggcgga agaccattca aaatctggag aaggcgcgac
     9601 agccacgtcg ccagatcggc accctggatg tggcgttcaa gaagcgcttc ctgaaactca
     9661 ccgccgatgc ggagaacaat ccggccacgc ccattctcac ccgccgcctg acaatgcggg
     9721 cggagaccat ccgggcactg gaggtgcgta agaattcggt gatggccgaa aggcggaagt
     9781 ccgcgatgca aactctggga gcaaagaacg agtacggaat cacaactgga gcaccgatta
     9841 acaacaatgg agctctgccg aatcagcgaa gcggacgtgt ctcgaatgca ggcatcagca
     9901 tcaaggatgt attcaatgtg aacggtggtg gtgccgagca gatttacggc tcgaatgggg
     9961 gcggcactat caaccagggt tacgagcatg tgatcgacga ggacggcgac ggcaactccc
    10021 tgcggctgac caccaggaat ccccatcccc acccgcacca ccaggtctcc tggggccaga
    10081 acaccaacgg cggcggcggt aatggcacag gtcgcctgtc cggcttcgcc aacgagctgg
    10141 gcccccgcct gatgggcaag catcaccatc atcaccacta atagtctaga ggatctttgt
    10201 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
    10261 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
    10321 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
    10381 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
    10441 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
    10501 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
    10561 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
    10621 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
    10681 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
    10741 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10861 tcatcaatgt atcttatcat gtctggatcc actagtgtcg acgatgtagg tcacggtctc
    10921 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
    10981 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
    11041 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
    11101 gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac
    11161 ggtcacggcg ggcatgtcga cactagttct agccagcttt tgttcccttt agtgagggtt
    11221 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    11281 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    11341 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    11401 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    11461 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    11521 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11581 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11641 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11701 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11761 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11821 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11881 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11941 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12001 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12061 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    12121 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12181 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12241 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    12301 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    12361 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    12421 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    12481 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    12541 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12601 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    12661 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12721 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12781 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    12841 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    12901 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    12961 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    13021 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    13081 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    13141 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    13201 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    13261 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    13321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    13381 ccgaaaagtg ccac
//
