LOCUS       sequence-268603-       10045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 
            fused to MS2 binding protein, and hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Olivero CE, Martinez-Terroba E, Zimmer J, Liao C, Tesfaye E, 
            Hooshdaran N, Schofield JA, Bendor J, Fang D, Simon MD, Zamudio JR, 
            Dimitrova N
  TITLE     p53 Activates the Long Noncoding RNA Pvt1b to Inhibit Myc and 
            Suppress Tumorigenesis.
  JOURNAL   Mol Cell. 2020 Feb 20;77(4):761-774.e8. doi: 
            10.1016/j.molcel.2019.12.014. Epub 2020 Jan 20.
  PUBMED    31973890
REFERENCE   2  (bases 1 to 10045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.12.014"; journalName: "Mol Cell"; date: 
            "2020-02-20- 20"; volume: "77"; issue: "4"; pages: "761-774.e8"
COMMENT     Sequence Label: lenti-SAM-hygro
FEATURES             Location/Qualifiers
     source          1..10045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1685..1918
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2103..2147
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2445..2562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2655..2903
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2655..2675
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2826..2845
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2941..2959
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        3011..3029
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        3177..4355
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3408..4346
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4303..4323
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4362..4371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4371..4757
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4812..4832
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4848..5390
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             5415..5786
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             5802..5855
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5856..6881
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5856..6881
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    6934..7522
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6987..7007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7405..7416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7525..7541)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7526..7542)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8051..8231
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8293..8881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8373..8392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9052..9843,9844..9912))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9675..9694
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9913..10017)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2341 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2401 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2461 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2521 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2581 agcagagatc cagtttggtt agtaccgggc ccgggtcgac tagtgatcag tgtgagggag
     2641 tgtaaagctg gtttgagggc ctatttccca tgattccttc atatttgcat atacgataca
     2701 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
     2761 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     2821 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     2881 tcttgtggaa aggacgaaac accggagacg ggataccgtc tctgttttag agctaggcca
     2941 acatgaggat cacccatgtc tgcagggcct agcaagttaa aataaggcta gtccgttatc
     3001 aacttggcca acatgaggat cacccatgtc tgcagggcca agtggcaccg agtcggtgct
     3061 ttttttaaac cgctgttcct aggaatcccg aggccttgca aagatggata aagttttaaa
     3121 cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct
     3181 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     3241 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     3301 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     3361 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     3421 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     3481 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     3541 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     3601 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     3661 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     3721 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     3781 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     3841 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     3901 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     3961 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     4021 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     4081 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     4141 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     4201 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     4261 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     4321 ttcaaagttt ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg gcttcaaact
     4381 ttactcagtt cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta
     4441 atttcgctaa tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg
     4501 tgacatgcag cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg
     4561 tccccaaagt ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt
     4621 cctacctgaa catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca
     4681 tcgtgaaggc aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg
     4741 ctaactcagg tatctacagc gctggaggag gtggaagcgg aggaggagga agcggaggag
     4801 gaggtagcgg acctaagaaa aagaggaagg tggcggccgc tggatcccct tcagggcaga
     4861 tcagcaacca ggccctggct ctggccccta gctccgctcc agtgctggcc cagactatgg
     4921 tgccctctag tgctatggtg cctctggccc agccacctgc tccagcccct gtgctgaccc
     4981 caggaccacc ccagtcactg agcgctccag tgcccaagtc tacacaggcc ggcgagggga
     5041 ctctgagtga agctctgctg cacctgcagt tcgacgctga tgaggacctg ggagctctgc
     5101 tggggaacag caccgatccc ggagtgttca cagatctggc ctccgtggac aactctgagt
     5161 ttcagcagct gctgaatcag ggcgtgtcca tgtctcatag tacagccgaa ccaatgctga
     5221 tggagtaccc cgaagccatt acccggctgg tgaccggcag ccagcggccc cccgaccccg
     5281 ctccaactcc cctgggaacc agcggcctgc ctaatgggct gtccggagat gaagacttct
     5341 caagcatcgc tgatatggac tttagtgccc tgctgtcaca gatttcctct agtgggcagg
     5401 gaggaggtgg aagcggcttc agcgtggaca ccagtgccct gctggacctg ttcagcccct
     5461 cggtgaccgt gcccgacatg agcctgcctg accttgacag cagcctggcc agtatccaag
     5521 agctcctgtc tccccaggag ccccccaggc ctcccgaggc agagaacagc agcccggatt
     5581 cagggaagca gctggtgcac tacacagcgc agccgctgtt cctgctggac cccggctccg
     5641 tggacaccgg gagcaacgac ctgccggtgc tgtttgagct gggagagggc tcctacttct
     5701 ccgaagggga cggcttcgcc gaggacccca ccatctccct gctgacaggc tcggagcctc
     5761 ccaaagccaa ggaccccact gtctccgcta gcggcagtgg agagggcaga ggaagtctgc
     5821 taacatgcgg tgacgtcgag gagaatcctg gcccaatgaa aaagcctgaa ctcaccgcga
     5881 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt atccgacctg atgcagctct
     5941 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     6001 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     6061 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     6121 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     6181 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     6241 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     6301 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     6361 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     6421 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     6481 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     6541 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     6601 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     6661 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     6721 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     6781 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     6841 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gaaacgttgt tgtttggggt
     6901 tgaattactc ttctagaccg cggcgcgccc gataatcaac ctctggatta caaaatttgt
     6961 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7021 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7081 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7141 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7201 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7261 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7321 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7381 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7441 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7501 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca
     7561 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     7621 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     7681 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7741 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     7801 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     7861 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     7921 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     7981 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     8041 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8101 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8161 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8221 aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8281 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8341 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8701 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8761 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8821 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8881 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8941 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9001 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9061 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9121 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9181 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9241 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9301 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9361 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9421 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9481 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9541 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9601 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9661 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9721 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9781 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9841 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9901 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9961 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10021 atttccccga aaagtgccac ctgac
//
