LOCUS       sequence-268605-        9168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for constitutive expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin 
            DC, Madison BB
  TITLE     The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and 
            a Wnt-independent mechanism in colorectal cancer.
  JOURNAL   Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022.
  PUBMED    37310750
REFERENCE   2  (bases 1 to 9168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022."
COMMENT     Sequence Label: BII-ChBtW-CTNNB1_S33Y
FEATURES             Location/Qualifiers
     source          1..9168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(308..324)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..324)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      1257..1266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1263..1658
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1677..1730
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1740..1763
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1776..4121
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDYGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     misc_feature    4144..4732
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4197..4217)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4615..4626)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4812..4946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4836..4855)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4890..4909
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5725..5787
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5917..5934)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5917..5933)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5926..5948)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6075..6530
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6162..6181)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6372..6393
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6556..6660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6661..6729,6730..7521)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6879..6898)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7692..8280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8181..8200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8434..8451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8568..8589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8604..8609,8610..8627,8628..8634)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8642..8658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8647..8669
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8666..8682
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8666..8682
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ccactagttc tagagggaca gccccccccc aaagccccca gggatgtaat
      361 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
      421 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
      481 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
      541 ggatacgggg aaaaggcctc caaggcctgc aggagtcaat gggaaaaacc cattggagcc
      601 aagtacactg actcaatagg gactttccat tgggttttgc ccagtacata aggtcaatag
      661 ggggtgagtc aacaggaaag tcccattgga gccaagtaca ttgagtcaat agggactttc
      721 caatgggttt tgcccagtac ataaggtcaa tgggaggtaa gccaatgggt ttttcccatt
      781 actgacatgt atactgagtc attagggact ttccaatggg ttttgcccag tacataaggt
      841 caataggggt gaatcaacag gaaagtccca ttggagccaa gtacactgag tcaataggga
      901 ctttccattg ggttttgccc agtacaaaag gtcaataggg ggtgagtcaa tgggtttttc
      961 ccattattgg cacatacata aggtcaatag gggtgactag tggagaagag catgcttgag
     1021 ggctgagtgc ccctcagtgg gcagagagca catggcccac agtccctgag aagttggggg
     1081 gaggggtggg caattgaact ggtgcctaga gaaggtgggg cttgggtaaa ctgggaaagt
     1141 gatgtggtgt actggctcca cctttttccc cagggtgggg gagaaccata tataagtgca
     1201 gtagtctctg tgaacattcg gtttagtgaa cctgcaggtc ggccgccacg accggtgcca
     1261 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     1321 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     1381 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     1441 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     1501 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     1561 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     1621 ttcgtgaatt gctgccctct ggttatgtgt gggagggcgc tagccttggc tccggcgagg
     1681 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca ctcggcatgg
     1741 actacaagga cgacgatgac aagctcgata gcggcatggc tactcaagct gatttgatgg
     1801 agttggacat ggccatggaa ccagacagaa aagcggctgt tagtcactgg cagcaacagt
     1861 cttacctgga ctatggaatc cattctggtg ccactaccac agctccttct ctgagtggta
     1921 aaggcaatcc tgaggaagag gatgtggata cctcccaagt cctgtatgag tgggaacagg
     1981 gattttctca gtccttcact caagaacaag tagctgatat tgatggacag tatgcaatga
     2041 ctcgagctca gagggtacga gctgctatgt tccctgagac attagatgag ggcatgcaga
     2101 tcccatctac acagtttgat gctgctcatc ccactaatgt ccagcgtttg gctgaaccat
     2161 cacagatgct gaaacatgca gttgtaaact tgattaacta tcaagatgat gcagaacttg
     2221 ccacacgtgc aatccctgaa ctgacaaaac tgctaaatga cgaggaccag gtggtggtta
     2281 ataaggctgc agttatggtc catcagcttt ctaaaaagga agcttccaga cacgctatca
     2341 tgcgttctcc tcagatggtg tctgctattg tacgtaccat gcagaataca aatgatgtag
     2401 aaacagctcg ttgtaccgct gggaccttgc ataacctttc ccatcatcgt gagggcttac
     2461 tggccatctt taagtctgga ggcattcctg ccctggtgaa aatgcttggt tcaccagtgg
     2521 attctgtgtt gttttatgcc attacaactc tccacaacct tttattacat caagaaggag
     2581 ctaaaatggc agtgcgttta gctggtgggc tgcagaaaat ggttgccttg ctcaacaaaa
     2641 caaatgttaa attcttggct attacgacag actgccttca aattttagct tatggcaacc
     2701 aagaaagcaa gctcatcata ctggctagtg gtggacccca agctttagta aatataatga
     2761 ggacctatac ttacgaaaaa ctactgtgga ccacaagcag agtgctgaag gtgctatctg
     2821 tctgctctag taataagccg gctattgtag aagctggtgg aatgcaagct ttaggacttc
     2881 acctgacaga tccaagtcaa cgtcttgttc agaactgtct ttggactctc aggaatcttt
     2941 cagatgctgc aactaaacag gaagggatgg aaggtctcct tgggactctt gttcagcttc
     3001 tgggttcaga tgatataaat gtggtcacct gtgcagctgg aattctttct aacctcactt
     3061 gcaataatta taagaacaag atgatggtct gccaagtggg tggtatagag gctcttgtgc
     3121 gtactgtcct tcgggctggt gacagggaag acatcactga gcctgccatc tgtgctcttc
     3181 gtcatctgac cagccgacac caagaagcag agatggccca gaatgcagtt cgccttcact
     3241 atggactacc agttgtggtt aagctcttac acccaccatc ccactggcct ctgataaagg
     3301 ctactgttgg attgattcga aatcttgccc tttgtcccgc aaatcatgca cctttgcgtg
     3361 agcagggtgc cattccacga ctagttcagt tgcttgttcg tgcacatcag gatacccagc
     3421 gccgtacgtc catgggtggg acacagcagc aatttgtgga gggggtccgc atggaagaaa
     3481 tagttgaagg ttgtaccgga gcccttcaca tcctagctcg ggatgttcac aaccgaattg
     3541 ttatcagagg actaaatacc attccattgt ttgtgcagct gctttattct cccattgaaa
     3601 acatccaaag agtagctgca ggggtcctct gtgaacttgc tcaggacaag gaagctgcag
     3661 aagctattga agctgaggga gccacagctc ctctgacaga gttacttcac tctaggaatg
     3721 aaggtgtggc gacatatgca gctgctgttt tgttccgaat gtctgaggac aagccacaag
     3781 attacaagaa acggctttca gttgagctga ccagctctct cttcagaaca gagccaatgg
     3841 cttggaatga gactgctgat cttggacttg atattggtgc ccagggagaa ccccttggat
     3901 atcgccagga tgatcctagc tatcgttctt ttcactctgg tggatatggc caggatgcct
     3961 tgggtatgga ccccatgatg gaacatgaga tgggtggcca ccaccctggt gctgactatc
     4021 cagttgatgg gctgccagat ctggggcatg cccaggacct catggatggg ctgcctccag
     4081 gtgacagcaa tcagctggcc tggtttgata ctgacctgta atgctagagt tcgcgtctgg
     4141 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4201 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4261 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4321 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4381 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4441 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4501 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4561 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4621 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4681 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     4741 aattctggct aagcacttcg tggccgtcga tcgtttaaag ggaggtagtg agtcgagata
     4801 tcgatactag tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     4861 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4921 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     4981 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     5041 ctgattatga tcctctagag ggacagcccc cccccaaagc ccccagggat gtaattacgt
     5101 ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc
     5161 ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg
     5221 gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata
     5281 cggggaaaaa gccataccaa tggagctcac ggggacagcc cccccccaaa gcccccaggg
     5341 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     5401 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     5461 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     5521 cctgggggga tacggggaaa aagaattcga taaaagtttt gttactttat agaagaaatt
     5581 ttgagttttt gtttttttta ataaataaat aaacataaat aaattgtttg ttgaatttat
     5641 tattagtatg taagtgtaaa tataataaaa cttaatatct attcaaatta ataaataaac
     5701 ctcgatatac agaccgataa aacacatgcg tcaattttac acatgattat ctttaacgta
     5761 cgtcacaata tgattatctt tctagggtta atctagctgc gtgttctgca gcgtgtcgag
     5821 catcttcatc tgctccatca cgctgtaaaa cacatttgca ccgcgagtct gcccgtcctc
     5881 cacgggttca aaaacgtgaa tgaacgaggc gcgctcactg gccgtcgttt tacaacgtcg
     5941 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     6001 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     6061 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6121 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6181 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6241 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6301 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6361 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6421 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     6481 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     6541 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6601 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6661 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6721 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6781 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6841 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6901 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6961 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7021 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7081 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7141 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7201 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7261 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7321 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7381 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7441 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7501 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7561 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7801 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8281 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8341 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8401 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8461 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     8521 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8581 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8641 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     8701 gcccgccggg taactcacgg ggtatccatg tccatttctg cggcatccag ccaggatacc
     8761 cgtcctcgct gacgtaatat cccagcgccg caccgctgtc attaatctgc acaccggcac
     8821 ggcagttccg gctgtcgccg gtattgttcg ggttgctgat gcgcttcggg ctgaccatcc
     8881 ggaactgtgt ccggaaaagc cgcgacgaac tggtatccca ggtggcctga acgaacagtt
     8941 caccgttaaa ggcgtgcatg gccacacctt cccgaatcat catggtaaac gtgcgttttc
     9001 gctcaacgtc aatgcagcag cagtcatcct cggcaaactc tttccatgcc gcttcaacct
     9061 cgcgggaaaa ggcacgggct tcttcctccc cgatgcccag atagcgccag cttgggcgat
     9121 gactgagccg gaaaaaagac ccgacgatat gatcctgatg cagctaga
//
