LOCUS       sequence-26862-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Steigemann P, Wurzenberger C, Schmitz MH, Held M, Guizetti J, Maar 
            S, Gerlich DW
  TITLE     Aurora B-mediated abscission checkpoint protects against 
            tetraploidization.
  JOURNAL   Cell. 2009 Feb 6. 136(3):473-84.
  PUBMED    19203582
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Feb 6. 136(3):473-84."
COMMENT     Sequence Label: pMyrPalm_mEGFP_IRES_puro2b
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(114..133)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(115..133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(178..381)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(207..227)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        382..756
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 caccggggan gctcctcgcc cttgctcacc ttcatgtcca cgccgtcgtc gttcaggttg
       61 tccttgcgct tgctcttgat gcagcccatg gtggcggccg caagcttggg tctccctata
      121 gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta gccagagagc
      181 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      241 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      301 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
      361 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
      421 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
      481 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
      541 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
      601 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
      661 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
      721 gtattgatac tattataact agtcnatatc aatgtcaacg cgtaatctgg gccgtactcc
      781 cnaacacgca aacgcctaac ctaacanatc tctgcattgt cgtcagcctg ctgtgactaa
      841 atttgtcccc tancacactc acaacagngg acaacg
//
