LOCUS       sequence-268626-        9001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human p115-RhoGEF with N-terminal EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   de Carcer G, Wachowicz P, Martinez-Martinez S, Oller J, 
            Mendez-Barbero N, Escobar B, Gonzalez-Loyola A, Takaki T, El Bakkali
            A, Camara JA, Jimenez-Borreguero LJ, Bustelo XR, Canamero M, Mulero 
            F, de Los Angeles Sevilla M, Montero MJ, Redondo JM, Malumbres M
  TITLE     Plk1 regulates contraction of postmitotic smooth muscle cells and is
            required for vascular homeostasis.
  JOURNAL   Nat Med. 2017 Aug;23(8):964-974. doi: 10.1038/nm.4364. Epub 2017 Jul
            10.
  PUBMED    28692064
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nm.4364"; 
            journalName: "Nat Med"; date: "2017-08"; volume: "23"; issue: "8"; 
            pages: "964-974"
COMMENT     Sequence Label: pDEST-EGFP_Hu_p115-RhoGEF(WT)
FEATURES             Location/Qualifiers
     source          1..9001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(898..900,901..903,904..1614)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(898..900,901..903,904..1614)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(943..964)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1204..1223)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1551..1572
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1678..1702
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4478..4502)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4554..4572)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4555..4572)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4592..4609)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4598..4822
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4868..5296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4955..4974)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5165..5186
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5305..5325)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5310..5639
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5490..5625
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5552..5571
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5706..6500
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5760..5779)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6370..6389
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6674..6795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6711..6730)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6765..6784
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6844..6860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6844..6860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6857..6879)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6868..6884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6892..6898,6899..6916,6917..6922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6937..6958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7075..7092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7246..7834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7326..7345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8005..8796,8797..8865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8628..8647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8866..8970)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttaccatg
      901 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      961 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1021 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1081 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1141 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1201 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1261 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1321 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1381 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1441 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1501 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1561 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagcttggt
     1621 accgagctcg gatccactag taacggccgc cagtgtgctg gaattctgca gatatcaaca
     1681 agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccatggaaga cttcgcccga
     1741 ggggcggcct ccccaggccc ctcccggcct ggcctggttc ccgtcagcat catcggggct
     1801 gaggatgagg attttgagaa cgagctggag acaaactcag aagagcaaaa cagccagttc
     1861 cagagcctgg agcaggtgaa gcggcgccca gcccacctca tggccctcct gcagcacgtg
     1921 gccctgcagt ttgagccagg acccctgctt tgctgtctgc atgccgacat gctgggctca
     1981 ctgggcccca aggaggccaa gaaggccttc ctggacttct accacagctt cctggagaag
     2041 acagcggttc tccgggtgcc ggtccctccc aacgtcgcct ttgaacttga ccgcactagg
     2101 gctgacctca tctccgagga tgtccagcgg cggttcgtgc aggaggtggt gcaaagccag
     2161 caggtagccg tgggccggca gctggaggac ttccgttcca agcggctcat gggcatgacg
     2221 ccctgggagc aggagctggc ccagctggag gcttgggttg ggcgggaccg agccagctac
     2281 gaggcccggg agcggcacgt ggcggagcgg ctgctcatgc acctggagga gatgcaacat
     2341 accatctcta ccgacgaaga aaagagtgct gccgtggtca acgccattgg cctgtacatg
     2401 cgccaccttg gggtgcggac caagagtgga gacaagaagt cggggaggaa cttcttccgg
     2461 aaaaaggtga tggggaaccg gcggtcggac gagcctgcca agaccaagaa ggggctgagc
     2521 agcatcctgg atgccgcccg ctggaaccgg ggagagcccc aggttccaga ttttcgacac
     2581 ctcaaagcag aggttgatgc cgagaagcca ggtgctacag accggaaggg aggcgtgggg
     2641 atgccctctc gggaccggaa tatcggggct cctgggcagg acacccctgg agtctctctg
     2701 caccctctgt ccctggacag cccagaccgg gaaccaggtg ctgacgcccc cctggagctg
     2761 ggggactcat ccccgcaggg cccaatgagc ctggagtcct tggcgccccc agagagtacc
     2821 gacgaggggg ccgaaaccga gagccccgag cctggagatg agggggagcc ggggcggtcg
     2881 ggactggagc ttgaaccaga agagcctccc ggctggcggg aactcgtccc cccagacacc
     2941 ctgcacagcc tgcccaagag ccaggtgaag cggcaggagg tcatcagcga gctgctggtg
     3001 acagaggcgg cccacgtgcg catgctgcgg gtgctgcacg acctcttctt ccagcccatg
     3061 gcagaatgcc tgttcttccc cttggaggag ctgcagaaca tcttccccag cctggacgag
     3121 ctcatcgagg tgcattccct gttcctcgat cgcctgatga agcggaggca ggagagtggc
     3181 tacctcatcg aggagatcgg agacgtgctg ctggcccggt ttgatggtgc tgagggctcc
     3241 tggttccaga aaatctcctc ccgcttctgc agccgccagt catttgcctt agagcagctc
     3301 aaagccaagc aacgcaagga ccctcggttc tgtgccttcg tgcaggaagc tgagagccgc
     3361 ccgcggtgcc gccgcctgca gctgaaggac atgatcccca cggagatgca gcggctgacc
     3421 aagtaccccc tgctcctgca gagcatcggg cagaacacag aagagcccac agaacgggag
     3481 aaagtggagc tggcagccga gtgctgccgg gaaattctac accacgtcaa ccaagccgtg
     3541 cgtgacatgg aggacctgct gaggctcaag gactatcagc ggcgcctgga cttgtcccac
     3601 cttcggcaga gcagcgaccc tatgctgagc gagttcaaga acctggacat caccaagaag
     3661 aaattggtcc acgagggccc actgacgtgg cgggtgacta aggacaaggc agtggaggtg
     3721 catgtgctgc tgctggacga cctgctgctg ctgctccagc gccaggacga gcggctgctg
     3781 ctcaagtccc atagccggac actgacgccc acgcccgatg gcaagaccat gctgcggccc
     3841 gtgctgcggc tcacctccgc catgacccgc gaggtggcca ccgatcacaa agccttctac
     3901 gtccttttta cctgggacca ggaggcccag atatacgagc tggtggcaca gactgtgtcg
     3961 gagcggaaaa actggtgtgc tctcatcact gagactgccg gatccctgaa agtccctgcc
     4021 cctgcctctc gccctaagcc ccggcccagc ccgagcagca cccgagaacc cctcctcagc
     4081 agctctgaga acggcaatgg tggccgagag acgtctccag ctgatgcccg gaccgagaga
     4141 atcctcagtg acctcctgcc cttctgcaga ccaggccccg agggccagct cgctgccacg
     4201 gcccttcgga aagtgctgtc cctgaagcag cttctgtttc cggcggagga agacaatggg
     4261 gcggggcctc ctcgagatgg ggatggggtc ccagggggcg gccccctgag cccagcacgg
     4321 acccaggaaa tccaggagaa cctgctcagc ttggaggaga ccatgaagca gctggaggag
     4381 ttggaggagg aattttgccg cctgagaccc ctcctgtctc agcttggggg gaactctgtc
     4441 ccccagcctg gctgcacttg aaagggtggg cgcgccgacc cagctttctt gtacaaagtg
     4501 gttgatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc tattctatag
     4561 tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     4621 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4681 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4741 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4801 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4861 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4921 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4981 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5041 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5101 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5161 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5221 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5281 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     5341 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     5401 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5461 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5521 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     5581 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5641 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     5701 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5761 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5821 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5881 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5941 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6001 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6061 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6121 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6181 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     6241 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     6301 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     6361 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     6421 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     6481 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     6541 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6601 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6661 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6721 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6781 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6841 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6901 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6961 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7021 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7681 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7921 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7981 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8041 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8101 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8161 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8221 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8281 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8341 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8401 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8461 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8521 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8581 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8641 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8701 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8761 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8821 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8881 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8941 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9001 c
//
