LOCUS       sequence-268653-       10114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE043 (TUPV1), pJM451 (TUPV2), 
            pPD612.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 10114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE059 ZF43x1 mKate2 EF1a EBFP2-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..10114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3058..3067
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3064..3762
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3813..4037
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5086..6268
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5317..6259
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6216..6236
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6270..6294
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6369..6378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6375..6377,6378..6380,6381..7091)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(6420..6441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             7119..7175
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7188..7586
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7637..7861
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(8720..9529)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9443..9462
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9521..9540
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(9688..10114,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9768..9787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattagag tgaggacgat
     3001 ccctctagag ggtatataat gggggccact agtctactac cagagctcat cgctagcgcc
     3061 accatggtga gcgagctgat taaggagaac atgcacatga agctgtatat ggagggcacc
     3121 gtgaacaacc accacttcaa gtgtacctcc gagggcgaag gcaagcccta cgagggcacc
     3181 cagaccatga gaatcaaggc ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
     3241 gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg catccccgac
     3301 ttctttaagc agtccttccc cgagggcttc acatgggaga gagtcaccac atacgaagat
     3361 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
     3421 gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
     3481 ggctgggagg cctccaccga aaccctgtac cccgctgacg gcggcctgga aggcagagcc
     3541 gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttgaa aaccacatac
     3601 agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt ggacaggaga
     3661 ctggaaagaa tcaaggaggc cgacaaagaa acctacgtcg agcagcacga ggtggctgtg
     3721 gccagatact gcgacctccc tagcaaactg gggcacagat gagcggccgc tcgagtctag
     3781 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3841 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3901 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3961 tggggtgggg caggacagca agggggagga ttgggaagtc aatagcaggc atgctgggga
     4021 tgcggtgggc tctatggctt ctgaggcagg tcgagggtac atgcgcctta ctccttgtga
     4081 gcgcatcgcg ataagctcta gagggacagc ccccccccaa agcccccagg gatgtaatta
     4141 cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc
     4201 tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca
     4261 cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg
     4321 atacggggaa aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc
     4381 ctgcgtacgg atggggctct agctctagag cttgatatcg aattcctgca gccccgctag
     4441 agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
     4501 cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg
     4561 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac
     4621 gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg
     4681 ctgaaagaga gatttagaat gacagaatca tagaacggcc tgggcgcgcc tggtggcgaa
     4741 cgatcaacag acgggagagg gcaaaaccga tgttgacgga ctaatcctga caagatctgg
     4801 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     4861 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     4921 cagatatacg cgtgagtaat tcatacaaaa ggactcgccc ctgccttggg gaatcccagg
     4981 gaccgtcgtt aaactcccac taacgtagaa cccagagatc gctgcgttcc cgccccctca
     5041 cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc cggtgcccgt
     5101 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5161 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5221 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5281 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     5341 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacgcccctg
     5401 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     5461 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ttgggcgctg
     5521 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     5581 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     5641 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     5701 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     5761 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     5821 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     5881 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc
     5941 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     6001 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     6061 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     6121 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     6181 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     6241 tttttttctt ccatttcagg tgtcgtgaga gctcgtttag tgaaccgtca gatcgcctgg
     6301 agacgccatc cacgctgttt tgacctccat agaagagaac gggaccgatc caaattctcg
     6361 aggctagcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgaggggc gagggcgagg
     6481 gcgatgccac caacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     6541 tgccctggcc caccctcgtg accaccctga gccacggcgt gcagtgcttc gcccgctacc
     6601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6661 agcgcaccat cttcttcaag gacgacggca cctacaagac ccgcgccgag gtgaagttcg
     6721 agggcgacac cctagtgaac cgcatcgagc tgaagggcgt cgacttcaag gaggacggca
     6781 acatcctggg gcacaagctg gagtacaact tcaacagcca caacatctat atcatggccg
     6841 tcaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacgtg gaggacggca
     6901 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6961 tgcccgacag ccactacctg agcacccagt ccgtgctgag caaagacccc aacgagaagc
     7021 gcgatcacat ggtcctgctg gagttccgca ccgccgccgg gatcactctc ggcatggacg
     7081 agctgtacaa ggaattcggt agcaagcttg gtagtggagc aactaacttt tccctgctca
     7141 aacaagccgg ggatgtagag gagaaccccg gccccgcccc ccttaagatg gccaagcctt
     7201 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     7261 tctctgagga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     7321 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     7381 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     7441 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
     7501 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     7561 cctctggtta tgtgtgggag ggctaagcgg ccgctcgagt ctagagggcc cgtttaaacc
     7621 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7681 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7741 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7801 agcaaggggg aggattggga agtcaatagc aggcatgctg gggatgcggt gggctctatg
     7861 gcttctgagg caggtcgaag aactatttcc tggctgttac gcgagcgcat cgcgataagc
     7921 tctagaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
     7981 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
     8041 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     8101 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct
     8161 ttaggctgaa agagagattt agaatgacag aatcatagaa cggcctgcgt acggatgggg
     8221 ctctagctct agagcttgat atcgaattcc tgcagccccg ctagagggac agcccccccc
     8281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     8341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     8401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     8461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     8521 gaatgacaga atcatagaac ggcctgggcg cgcctggtgg cgaacgatca acagacggga
     8581 gaggactaga ggatgcacat gtgaccgagg gatggaacga cgcgtaactc acgttaaggg
     8641 attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca
     8701 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     8761 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     8821 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     8881 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     8941 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     9001 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     9061 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     9121 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     9181 tttcacctga atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag
     9241 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     9301 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     9361 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaag cgatagattg
     9421 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     9481 tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata acaccccttg
     9541 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatattatt ttatcttgtg
     9601 caatgtaaca tcagagattt tgagacacgg gccatgctag ctccagcaaa aggccaggaa
     9661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10081 tgctacagag ttcttgaagt ggtggcctaa ctac
//
