LOCUS       sequence-268655-       10195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE045 (TUPV1), pJM451 (TUPV2), 
            pPD612.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE061 ZF43x6-C mKate2 EF1a EBFP2-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..10195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3139..3148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3145..3843
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3894..4118
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5167..6349
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5398..6340
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6297..6317
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6351..6375
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6450..6459
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6456..6458,6459..6461,6462..7172)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(6501..6522)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             7200..7256
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7269..7667
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7718..7942
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(8801..9610)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9524..9543
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9602..9621
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(9769..10195,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9849..9868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattacga tcggagtgag
     3001 gataattaga gtgaggacga tcggagtgag gaaaattaga gtgaggacga tcggagtgag
     3061 gataattaga gtgaggacga tccctctaga gggtatataa tgggggccac tagtctacta
     3121 ccagagctca tcgctagcgc caccatggtg agcgagctga ttaaggagaa catgcacatg
     3181 aagctgtata tggagggcac cgtgaacaac caccacttca agtgtacctc cgagggcgaa
     3241 ggcaagccct acgagggcac ccagaccatg agaatcaagg cggtcgaggg cggccctctc
     3301 cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac
     3361 cacacccagg gcatccccga cttctttaag cagtccttcc ccgagggctt cacatgggag
     3421 agagtcacca catacgaaga tgggggcgtg ctgaccgcta cccaggacac cagcctccag
     3481 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct
     3541 gtgatgcaga agaaaacact cggctgggag gcctccaccg aaaccctgta ccccgctgac
     3601 ggcggcctgg aaggcagagc cgacatggcc ctgaagctcg tgggcggggg ccacctgatc
     3661 tgcaacttga aaaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc
     3721 gtctactatg tggacaggag actggaaaga atcaaggagg ccgacaaaga aacctacgtc
     3781 gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaga
     3841 tgagcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     3901 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3961 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4021 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaagt
     4081 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcag gtcgagggta
     4141 catgcgcctt actccttgtg agcgcatcgc gataagctct agagggacag ccccccccca
     4201 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
     4261 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc
     4321 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
     4381 agcctgcaga cacctggggg gatacgggga aaaagcttta ggctgaaaga gagatttaga
     4441 atgacagaat catagaacgg cctgcgtacg gatggggctc tagctctaga gcttgatatc
     4501 gaattcctgc agccccgcta gagggacagc ccccccccaa agcccccagg gatgtaatta
     4561 cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc
     4621 tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca
     4681 cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg
     4741 atacggggaa aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc
     4801 ctgggcgcgc ctggtggcga acgatcaaca gacgggagag ggcaaaaccg atgttgacgg
     4861 actaatcctg acaagatctg gggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     4921 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     4981 gctgcttcgc gatgtacggg ccagatatac gcgtgagtaa ttcatacaaa aggactcgcc
     5041 cctgccttgg ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat
     5101 cgctgcgttc ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat
     5161 gcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     5221 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     5281 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5341 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     5401 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     5461 tgaattactt ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     5521 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     5581 tgaggcctgg cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     5641 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     5701 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     5761 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     5821 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     5881 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     5941 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     6001 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     6061 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     6121 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     6181 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     6241 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     6301 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag agctcgttta
     6361 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagagaa
     6421 cgggaccgat ccaaattctc gaggctagcg ccaccatggt gagcaagggc gaggagctgt
     6481 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     6541 gcgtgagggg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct
     6601 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg agccacggcg
     6661 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     6721 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc acctacaaga
     6781 cccgcgccga ggtgaagttc gagggcgaca ccctagtgaa ccgcatcgag ctgaagggcg
     6841 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc
     6901 acaacatcta tatcatggcc gtcaagcaga agaacggcat caaggtgaac ttcaagatcc
     6961 gccacaacgt ggaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     7021 tcggcgacgg ccccgtgctg ctgcccgaca gccactacct gagcacccag tccgtgctga
     7081 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttccgc accgccgccg
     7141 ggatcactct cggcatggac gagctgtaca aggaattcgg tagcaagctt ggtagtggag
     7201 caactaactt ttccctgctc aaacaagccg gggatgtaga ggagaacccc ggccccgccc
     7261 cccttaagat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     7321 ctacaatcaa cagcatcccc atctctgagg actacagcgt cgccagcgca gctctctcta
     7381 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     7441 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     7501 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     7561 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     7621 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagcg gccgctcgag
     7681 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     7741 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     7801 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     7861 gggggtgggg tggggcagga cagcaagggg gaggattggg aagtcaatag caggcatgct
     7921 ggggatgcgg tgggctctat ggcttctgag gcaggtcgaa gaactatttc ctggctgtta
     7981 cgcgagcgca tcgcgataag ctctagaggg acagcccccc cccaaagccc ccagggatgt
     8041 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
     8101 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
     8161 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
     8221 gggggatacg gggaaaaagc tttaggctga aagagagatt tagaatgaca gaatcataga
     8281 acggcctgcg tacggatggg gctctagctc tagagcttga tatcgaattc ctgcagcccc
     8341 gctagaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
     8401 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
     8461 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     8521 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct
     8581 ttaggctgaa agagagattt agaatgacag aatcatagaa cggcctgggc gcgcctggtg
     8641 gcgaacgatc aacagacggg agaggactag aggatgcaca tgtgaccgag ggatggaacg
     8701 acgcgtaact cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg
     8761 taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca
     8821 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     8881 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     8941 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     9001 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     9061 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     9121 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     9181 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     9241 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     9301 ctgtttttcc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     9361 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     9421 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     9481 tcccatacaa gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     9541 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa
     9601 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     9661 atgatattat tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccatgcta
     9721 gctccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actac
//
