LOCUS       sequence-26870-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Steigemann P, Wurzenberger C, Schmitz MH, Held M, Guizetti J, Maar 
            S, Gerlich DW
  TITLE     Aurora B-mediated abscission checkpoint protects against 
            tetraploidization.
  JOURNAL   Cell. 2009 Feb 6. 136(3):473-84.
  PUBMED    19203582
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Feb 6. 136(3):473-84."
COMMENT     Sequence Label: pH2B_mCherry_IRES_neo3
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             96..473
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      486..495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(637..655)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagctc
       61 aagcttcgaa ttctgcagtc gacggtaccg ccaccatgcc agagccagcg aagtctgctc
      121 ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga
      181 agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg
      241 tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg
      301 acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga
      361 ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca
      421 agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagcgct aaggatccac
      481 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
      541 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      601 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      661 gccccctgcc cttcgcctgg gacatcctgt cccctcagt
//
