LOCUS       sequence-26874-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Steigemann P, Wurzenberger C, Schmitz MH, Held M, Guizetti J, Maar 
            S, Gerlich DW
  TITLE     Aurora B-mediated abscission checkpoint protects against 
            tetraploidization.
  JOURNAL   Cell. 2009 Feb 6. 136(3):473-84.
  PUBMED    19203582
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Feb 6. 136(3):473-84."
COMMENT     Sequence Label: pmRFP_LAP2beta_IRES_puro2b
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..720
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             46..720
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(176..194)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     444..463
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     638..661
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggc ctcctccgag
       61 gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac
      121 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      181 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
      241 cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
      301 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
      361 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
      421 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      481 tccaccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gatgaggctg
      541 aagctgaagg acggcggcca ctacgacgcc gaggtcaaga ccacctacat ggccaagaag
      601 cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc tggacatcac ctcccacaac
      661 gaggactaca ccatcgtgga acagtacgag cgcgccgagg gccgccactc caccggcgcc
      721 ctgtacaagt actcagatct cgagctcaag cttgcattaa
//
