LOCUS       sequence-268766-        9088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-controlled tri-directional targeting of endogenous proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Redchuk TA, Karasev MM, Verkhusha PV, Donnelly SK, Hulsemann M, 
            Virtanen J, Moore HM, Vartiainen MK, Hodgson L, Verkhusha VV
  TITLE     Optogenetic regulation of endogenous proteins.
  JOURNAL   Nat Commun. 2020 Jan 30;11(1):605. doi: 10.1038/s41467-020-14460-4.
  PUBMED    32001718
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-30"; volume: "11(1):605. doi"; pages: " 
            10.1038/s41467-020-14460-4"
COMMENT     Sequence Label: pQP-iRISB
FEATURES             Location/Qualifiers
     source          1..9088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      616..625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2910..2966
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    2968..3542
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2992..3544
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3136..3153)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3363..3382
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3637..3978
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     CDS             4000..4707
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4145..4163)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4413..4432
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5212..5226
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    5876..5997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5913..5932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5967..5986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6004..6459)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6141..6162)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6353..6372
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6486..6590
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6592..6949
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6592..6783
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6615..6635)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6800..6935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6984..7778
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6984..7778
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7038..7057)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7648..7667
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7966..7985)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8010..8057
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8386..8974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8875..8894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctaccatg gtggcaggtc atgcctctgg cagccccgca ttcgggaccg
      661 ccgatctttc gaattgcgaa cgtgaagaga tccacctcgc cggctcgatc cagccgcatg
      721 gcgcgcttct ggtcgtcagc gagccggatc atcgcatcat ccaggccagc gccaacgccg
      781 cggaatttct gaatctcgga agcgtgctcg gcgttccgct cgccgagatc gacggcgatc
      841 tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg gtcgcggtgc
      901 gctgccggat cggcaatccc tccacggagt acgacggtct gatgcatcgg cctccggaag
      961 gcgggctgat catcgagctc gaacgtgccg gcccgccgat cgatctgtcc ggcacgctgg
     1021 cgccggcgct ggagcggatc cgcacggcgg gctcgctgcg cgcgctgtgc gatgacaccg
     1081 cgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgc ttcgacgagc
     1141 agggccacgg cgaagtgttc tccgagcgcc acgtgcccgg gctcgaatcc tatttcggca
     1201 accgctatcc gtcgtcggac attccgcaga tggcgcggcg gctgtacgag cggcagcgcg
     1261 tccgcgtgct ggtcgacgtc agctatcagc cggtgccgct ggagccgcgg ctgtcgccgc
     1321 tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg ccgatccatc
     1381 tgcagtacct gaagaacatg ggcgtgcgcg ccaccctggt ggtgtcgctg gtggtcggcg
     1441 gcaagctgtg gggcctggtt gcctgtcacc attatctgcc gcgcttcatc catttcgagc
     1501 tgcgggcgat ctgcgaactg ctcgccgaag cgatcgcgac gcggatcacc gcgcttgaga
     1561 gcttcgcgca gagccagtcg gagctgttcg tgcagcggct cgaacagcgc atgatcgaag
     1621 cgatcacccg tgaaggcgat tggcgcgcag cgattttcga caccagccaa tcgatcctgc
     1681 agccgctgca cgccgacggt tgcgcgctgg tgtacgaaga ccagatcagg accatcggtg
     1741 acgtaccttc cacgcaggat gttcgcgaga tcgccgggtg gctcgatcgc cagccacgtg
     1801 cggcggtgac ctcgaccgcg tcgctcggtc tcgacgtgcc ggagctcgcg catctgacgc
     1861 ggatggcgag cggcgtggtc gcggcgccga tttcggatca tcgcggcgag tttctgatgt
     1921 ggttccgccc cgagcgcgtc cacaccgtta cctggggcgg cgatccgaag aagccgttca
     1981 cgatgggcga tacaccggcg gatctgtcgc cgcggcgctc cttcgccaaa tggcatcagg
     2041 ttgtcgaagg cacgtccgat ccgtggacgg ccgccgatct cgccgcggct cgcaccatcg
     2101 gtcagaccgt cgccgacatc gtgctgcaat tccgcgcggt gcggacactg atcgcccgcg
     2161 aacagtacga acagttttcg tcccaggtgc acgcttcgat gcagccggtg ctgatcaccg
     2221 acgccgaagg ccgcatcctg ctgatgaacg actcgttccg cgacatgttg ccggcggggt
     2281 cgccatccgc cgtccatctc gacgatctcg ccgggttctt cgtcgaatcg aacgatttcc
     2341 tgcgcaacgt cgccgaactg atcgatcacg gccgcgggtg gcgcggcgaa gttctgctgc
     2401 gcggcgcagg taatcgcccg ttgccgctgg cagtgcgcgc cgatccggtg acgcgcacgg
     2461 aggaccagtc gctcggcttc gtgctgatct tcagcgacgc taccgatcgt cgcaccgcag
     2521 atgccgcacg cacgcgtttc caggaaggca ttcttgccag cgcacgtccc ggcgtgcggc
     2581 tcgactccaa gtccgacctc ttgcacgaga agctgctgtc cgcgctggtc gagaacgcgc
     2641 agcttgccgc attggaaatt acttacggcg tcgagaccgg acgcatcgcc gagctgctcg
     2701 aaggcgttcg ccagtcgatg ctgcgcaccg ccgaagtgct cggccatctg gtgcagcacg
     2761 cggcgcgcac ggccggcagc gacagctcga gcaatggctc gcagaacaag aaggctagca
     2821 gtgctggcgg tagtgctggt agtgctggtc gtacggccag cggtaaaaag aagaaaaaga
     2881 agtcaaagac aaagtgtgta attatgtaaa gatctcgagc tcaagcttcg aattctgcag
     2941 tcgacggtac cgcgggcccg ggatccgccc ctctccctcc ccccccccct aacgttactg
     3001 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     3061 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     3121 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     3181 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     3241 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     3301 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     3361 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     3421 gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa
     3481 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     3541 aatatggcca caaaccggtc catgggaatt gatctctcag gattaacact gcagagcggc
     3601 ggagggagcg gcggatccgg agggggctct ggccaagtcc aactggtgga gtctggtggc
     3661 gctttggtgc agccaggtgg ctctctgcgt ttgtcctgtg ccgcttctgg cttcccagtg
     3721 aaccgctatt ccatgcgctg gtatcgccag gctccaggca aagagcgtga gtgggtagcc
     3781 ggtatgtcca gcgcgggtga tcgtagctcc tatgaagact ccgtgaaggg ccgtttcacc
     3841 atcagccgtg acgatgcccg taacacggtg tatctgcaaa tgaacagctt gaaacctgaa
     3901 gatacggccg tgtattactg taatgtgaac gtgggcttcg agtattgggg ccaaggcacc
     3961 caggtcaccg tctccagcgg aggcggatca tctactagta tggtgagcaa gggcgaggag
     4021 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     4081 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     4141 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     4201 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     4261 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     4321 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     4381 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     4441 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     4501 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     4561 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     4621 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     4681 accggcggca tggacgagct gtacaagagt gctggtgggt ccgccggcgg gagtgctggc
     4741 ggtagtgccg gcggtagttc tagaggcaag aacatgcagg cggtcaccga gctgcattcc
     4801 cggctgatcg ctgcgcagca ggcgatggag cgcgactatt ggcggttgcg tgaattggag
     4861 actcgctacc gcctggtgtt cgacgctgcc gccgatgcgg tgatgatcgt ctccgccggc
     4921 gacatgcgca tcgtcgaagc caaccgggcg gcggtgaatg cgatcagccg cgtcgagcgc
     4981 ggcaatgacg accttgcggg gcgtgatttc ctcgccgaag tggcggctgc cgatcgcgat
     5041 gcggtgcgcg acatgctggc ccaggtgcgt cagcgcggca ccgcactcag cgtcctcgtt
     5101 catctcggcc gttacgaccg cgcctggatg ctgcgcggtt cgctgatgtc gtccgagcgt
     5161 cgtcaggttt tcctgctgca cttcaccccg gtgaccacga ctcccgcgat cgacgacgac
     5221 gacaaaggtg tcgttgcttc tgctgccgac ggtgcagagg gcgcctcaga cgacgcagag
     5281 gatgaattcc cggctagcgg cgccggcgcc tttttggcta ctacacttga acgtattgag
     5341 aagaactttg tcattactga cccaaggttg ccagataatc ccattatatt cgcgtccgat
     5401 agtttcttgc agttgacaga atatagccgt gaagaaattt tgggaagaaa ctgcaggttt
     5461 ctacaaggtc ctgaaactga tcgcgcgaca gtgagaaaaa ttagagatgc catagataac
     5521 caaacagagg tcactgttca gctgattaat tatacaaaga gtggtaaaaa gttctggaac
     5581 ctctttcact tgcagcctat gcgagatcag aagggagatg tccagtactt tattggggtt
     5641 cagttggatg gaactgagca tgtccgagat gctgccgaga gagagggcgt tatgctggcc
     5701 aagaagaccg ccgagaacat cgacatggct gccaagaggg tcaagttgga ctgagcggcc
     5761 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5821 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5881 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5941 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     6001 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     6061 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     6121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     6181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     6241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     6301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     6361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     6421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     6481 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6541 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     6601 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6661 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6721 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6781 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6841 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6901 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     6961 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     7021 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     7081 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     7141 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     7201 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     7261 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     7321 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     7381 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     7441 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     7501 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     7561 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     7621 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     7681 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     7741 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     7801 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     7861 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7921 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     7981 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     8041 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     8101 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     8161 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     8221 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8281 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8341 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8521 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8761 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9001 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9061 tgtggataac cgtattaccg ccatgcat
//
