LOCUS       sequence-268888-       11218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HyPr plasmid with nptII marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Peris D, Alexander WG, Fisher KJ, Moriarty RV, Basuino MG, Ubbelohde
            EJ, Wrobel RL, Hittinger CT
  TITLE     Synthetic hybrids of six yeast species.
  JOURNAL   Nat Commun. 2020 Apr 29;11(1):2085. doi: 10.1038/s41467-020-15559-4.
  PUBMED    32350251
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-29"; volume: "11(1):2085. doi"; pages: " 
            10.1038/s41467-020-15559-4"
COMMENT     Sequence Label: pHRW40
FEATURES             Location/Qualifiers
     source          1..11218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    169..187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    211..229
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1569..1580
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2226..2723
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      2799..3046
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2815..2833)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3069..3085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3069..3085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3082..3104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3093..3109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3117..3123,3124..3141,3142..3147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3162..3183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3300..3317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3471..4059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3551..3570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4230..5021,5022..5090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4853..4872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5091..5195)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5263..5281
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     gene            5403..6759
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5403..5746
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5747..6556
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5814..5833)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6562..6759
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      7102..7939
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     misc_feature    8075..8367
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     primer_bind     9109..9131
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9123..9140
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9124..9140
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(9168..9554,9555..10175))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     10083..10102
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(10260..10284)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(10281..10484)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10310..10330)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10485..10864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(11029..11053)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagctcggt accctatggc atgcatgtgc tctgtatgta tataaaactc ttgttttctt
      301 cttttctcta aatattcttt ccttatacat taggtccttt gtagcataaa ttactatact
      361 tctatagaca cgcaaacaca aatacacaca ctaaattacc ggatcaattc gggggaaaaa
      421 tgctttctga aaacacgact attctgatgg ctaacggtga aattaaagac atcgcaaacg
      481 tcacggctaa ctcttacgtt atgtgcgcag atggctccgc tgcccgcgtc ataaatgtca
      541 cacagggcta tcagaaaatc tataatatac agcaaaaaac caaacacaga gcttttgaag
      601 gtgaacctgg taggttagat cccaggcgta gaacagttta tcagcgtctt gcattacaat
      661 gtactgcagg tcataaattg tcagtcaggg tccctaccaa accactgttg gaaaaaagtg
      721 gtagaaatgc caccaaatat aaagtgagat ggagaaatct gcagcaatgt cagacgcttg
      781 atggtaggat aataataatt ccaaaaaacc atcataagac attcccaatg acagttgaag
      841 gtgagtttgc cgcaaaacgc ttcatagaag aaatggagcg ctctaaagga gaatatttca
      901 actttgacat tgaagttaga gatttggatt atcttgatgc tcaattgaga atttctagct
      961 gcataagatt tggtccagta ctcacaggaa atggtgtttt atctaaattt ctcactggac
     1021 gtagtgacct tgtaactcct gctgtaaaaa gtatggcttg gatgcttggt ctgtggttag
     1081 gtgacggtac aacaaaagag ccagaaatct cagtagatag cttggatcct aagctaatgg
     1141 agagtttaag agaaaatgcg aaaatctggg gtctctacct tacggtttgt gacgatcacg
     1201 ttccgctacg tgccaaacat gtaaggcttc attatggaga tggtccagat gaaaacagga
     1261 agacaaggaa tttgaggaaa aataatccat tctggaaagc tgtcacaatt ttaaagttta
     1321 aaagggatct tgatggagag aagcaaatcc ctgaatttat gtacggcgag catatagaag
     1381 ttcgtgaagc attcttagcc ggcttgatcg actcagatgg gtacgttgtg aaaaagggcg
     1441 aaggccctga atcttataaa atagcaattc aaactgttta ttcatccatt atggacggaa
     1501 ttgtccatat ttcaagatct cttggtatgt cagctactgt gacgaccagg tcagctaggg
     1561 aggaaatcat tgaaggaaga aaagtccaat gtcaatttac atacgactgt aatgttgctg
     1621 ggggaacaac tttacagaat gttttgtcat attgtcgaag tggtcacaaa acaagagaag
     1681 ttccgccaat tataaaaagg gaacccgtat atttcagctt cacggatgat ttccagggtg
     1741 agagtactgt atatgggctt acgatagaag gccataaaaa tttcttgctt ggcaacaaaa
     1801 tagaagtgaa atcatgtcga ggctgctgtg tgggagaaca gcataaaata tcacaaaaaa
     1861 agaatctaaa acactgtgtt gcttgtccca gaaagggaat caagtatttt tataaagatt
     1921 ggagtggtaa aaatcgagta tgtgctagat gctatggaag atacaaattc agcggtcatc
     1981 actgtataaa ttgcaagtat gtaccagaag cacgtgaagt gaaaaaggca aaagacaaag
     2041 gcgaaaaatt gggcattacg cccgaaggtt tgccagttaa aggaccagag tgtataaaat
     2101 gtggcggaat cttacagttt gatgctgtcc gcgggcctca taagagttgt ggtaacaacg
     2161 caggtgcgcg catctgccgg atccccgggt taattaacgg tggaggtgga ggtggaggtg
     2221 gaggtgaaca aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag
     2281 aagaagactt gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg
     2341 gtgaacaaaa gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag
     2401 aagacttgaa cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg
     2461 aacaaaagct aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag
     2521 acttgaacgg actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac
     2581 aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact
     2641 tgaacggact cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa
     2701 agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatcaatcac tagtgaattc
     2761 gcgccacttc taaataagcg aatttcttga gggccgcatc atgtaattag ttatgtcacg
     2821 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
     2881 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
     2941 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
     3001 aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcggcc aagcttggcg
     3061 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3121 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     3181 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     3241 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3301 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3361 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3421 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3481 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3541 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3601 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3661 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3721 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3781 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3841 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3901 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3961 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4021 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4081 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4141 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4201 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4261 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4321 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4381 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4441 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4501 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4561 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4621 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4681 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4741 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4801 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4861 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4921 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4981 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5041 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5101 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5161 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5221 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     5281 ccctttcgtc ttcaagaatt cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg
     5341 gttaattaag gcgcgccaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca
     5401 gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat
     5461 gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca
     5521 tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg
     5581 agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga
     5641 gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa
     5701 tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg gtaaggaaaa
     5761 gactcacgtt tcgaggccgc gattgaattc caacatggat gctgatttat atgggtataa
     5821 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
     5881 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     5941 tgagatggtc agactaaact ggctgacgga attcatgcct cttccgacca tcaagcattt
     6001 tatccgtact cctgatgatg catggttact caccactgcg atccccggca aaacagcatt
     6061 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     6121 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     6181 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     6241 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
     6301 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     6361 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     6421 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     6481 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     6541 cgatgagttt ttctaatcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca
     6601 tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat
     6661 attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata
     6721 tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc
     6781 cgccatccag tgtcgaaaac gagctcgaat tcatcgatga tatcagatcc actagtggcc
     6841 tatgcaattc tcatgtttga cagcttatca tcgatcgtcc aactgcatgg agatgagtcg
     6901 tggcaagaat accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa
     6961 gactgcaaca tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt
     7021 gattcagaag caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt
     7081 ggaaggcaag agagccccga gagcttacat tttatgttag ctggtggact gacgccagaa
     7141 aatgttggtg atgcgcttag attaaatggc gttattggtg ttgatgtaag cggaggtgtg
     7201 gagacaaatg gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa
     7261 taggttatta ctgagtagta tttatttaag tattgtttgt gcacttgcct gcaagccttt
     7321 tgaaaagcaa gcataaaaga tctaaacata aaatctgtaa aataacaaga tgtaaagata
     7381 atgctaaatc atttggcttt ttgattgatt gtacaggaaa atatacatcg cagggggttg
     7441 acttttacca tttcaccgca atggaatcaa acttgttgaa gagaatgttc acaggcgcat
     7501 acgctacaat gacccgattc ttgctagcct tttctcggtc ttgcaaacaa ccgccggcag
     7561 cttagtatat aaatacacat gtacatacct ctctccgtat cctcgtaatc attttcttgt
     7621 atttatcgtc ttttcgctgt aaaaacttta tcacacttat ctcaaataca cttattaacc
     7681 gcttttacta ttatcttcta cgctgacagt aatatcaaac agtgacacat attaaacaca
     7741 gtggtttctt tgcataaaca ccatcagcct caagtcgtca agtaaagatt tcgtgttcat
     7801 gcagatagat aacaatctat atgttgataa ttagcgttgc ctcatcaatg cgagatccgt
     7861 ttaaccggac cctagtgcac ttaccccacg ttcggtccac tgtgtgccga acatgctcct
     7921 tcactatttt aacatgtgga attatcgatg ccggtgttat tcttgctatg ataggccatc
     7981 gaatgttaaa gggaagattt attgttttgc cctttaaaga cgaatttgga ccgtgaatgt
     8041 ttaaataatt ctcatgtttg acagcttatc atcgaactct aagaggtgat acttatttac
     8101 tgtaaaactg tgacgataaa accggaagga agaataagaa aactcgaact gatctataat
     8161 gcctattttc tgtaaagagt ttaagctatg aaagcctcgg cattttggcc gctcctaggt
     8221 agtgcttttt ttccaaggac aaaacagttt ctttttcttg agcaggtttt atgtttcggt
     8281 aatcataaac aataaataaa ttatttcatt tatgtttaaa aataaaaaat aaaaaagtat
     8341 tttaaatttt taaaaaagtt gattataagc atgtgacctt ttgcaagcaa ttaaattttg
     8401 caatttgtga tttaggcaaa agttactatt tctggctcgt gtaatatatg tatgctaatg
     8461 tgaactttta caaagtcgat atggacttag tcaaaagaaa ttttcttaaa aatatatagc
     8521 actagccaat ttagcacttc tttatgagat atattataga ctttattaag ccagatttgt
     8581 gtattatatg tatttacccg gcgaatcatg gacatacatt ctgaaatagg taatattctc
     8641 tatggtgaga cagcatagat aacctaggat acaagttaaa agctagtact gttttgcagt
     8701 aatttttttc ttttttataa gaatgttacc acctaaataa gttataaagt caatagttaa
     8761 gtttgatatt tgattgtaaa ataccgtaat atatttgcat gatcaaaagg ctcaatgttg
     8821 actagccagc atgtcaacca ctatattgat caccgatatt aggacttcca caccaactag
     8881 taatatgaca ataaattcaa gatattcttc atgagaatgg cccagcgatt atgcggtgtg
     8941 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     9001 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     9061 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     9121 gttgtaaaac gacggccagt gaattcttat tacgatcctc gcgcccccta cccaccgtac
     9181 tcgtcaattc caagggcatc ggtaaacatc tgctcaaact cgaagtcggc catatccaga
     9241 gcgccgtagg gggcggagtc gtggggggta aatcccggac ccggggaatc cccgtccccc
     9301 aacatgtcca gatcgaaatc gtctagcgcg tcggcatgcg ccatcgccac gtcctcgccg
     9361 tctaagtgga gctcgtcccc caggctgaca tcggtcgggg gggccgtcga cagtctgcgc
     9421 gtgtgtcccg cggggagaaa ggacaggcgc ggagccgcca gccccgcctc ttcgggggcg
     9481 tcgtcgtccg ggagatcgag caggccctcg atggtagacc cgtaattgtt tttcgtacgc
     9541 gcgcggctgt acgcggaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc
     9601 aattcaaggc cgaataagaa ggctggctct gcaccttggc gatcaaataa ttcgatagct
     9661 tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact
     9721 tgatgctctt gttcttccaa tacgcaacct aaagtaaagt gccccacagc gctgagtgca
     9781 tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga
     9841 gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga
     9901 cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc
     9961 ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggg taaggcgtcg
    10021 agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc
    10081 tgggcgagtt tacgggttgt taaaccttcg attccgaccc cattaagcag ctctaatgcg
    10141 ccgttaatca ctttactttt atctaatcta gacatatgaa ttaattcggg gccgcggagg
    10201 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
    10261 atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg
    10321 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
    10381 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
    10441 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
    10501 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
    10561 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
    10621 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
    10681 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
    10741 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
    10801 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
    10861 tgtcaacatg gcggtaatgt tggacatgag ccaatataaa tgtacatatt atgatatgga
    10921 tacaacgtat gcaatgggcc aagctcctcg agtaattcgc gccacttcta ataagcgatt
    10981 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
    11041 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
    11101 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
    11161 gcagatccgc tagggataac agggtaatat agatcaattc ctcgatcgag tttaccac
//
