LOCUS       sequence-268890-       11596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HyPr plasmid with hphMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Peris D, Alexander WG, Fisher KJ, Moriarty RV, Basuino MG, Ubbelohde
            EJ, Wrobel RL, Hittinger CT
  TITLE     Synthetic hybrids of six yeast species.
  JOURNAL   Nat Commun. 2020 Apr 29;11(1):2085. doi: 10.1038/s41467-020-15559-4.
  PUBMED    32350251
REFERENCE   2  (bases 1 to 11596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-29"; volume: "11(1):2085. doi"; pages: " 
            10.1038/s41467-020-15559-4"
COMMENT     Sequence Label: pHMK34
FEATURES             Location/Qualifiers
     source          1..11596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2123..2620
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     CDS             2240..2737
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      2813..3060
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2829..2847)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3083..3099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3083..3099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3096..3118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3107..3123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3131..3137,3138..3155,3156..3161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3176..3197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3314..3331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3485..4073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3565..3584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4244..5035,5036..5104))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4867..4886
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5105..5209)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5277..5295
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     gene            5417..6992
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        5417..5760
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5761..6789
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             5761..6789
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      6795..6992
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      7335..8172
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     misc_feature    8308..8600
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     primer_bind     9342..9364
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9356..9373
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9357..9373
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(9401..9787,9788..10408))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     10316..10335
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(10493..10517)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(10514..10717)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10543..10563)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10718..11097
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(11262..11286)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    join(11452..11470,11471..11493,11494..11512,11513..11535,
                     11536..11554,11555..11577,11578..11596,1..23,24..42,43..65,
                     66..84,85..107,108..126)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
       61 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      121 tagagaaaag tgaaagtcga gctcggtacc ctatggcatg catgtgctct gtatgtatat
      181 aaaactcttg ttttcttctt ttctctaaat attctttcct tatacattag gtcctttgta
      241 gcataaatta ctatacttct atagacacgc aaacacaaat acacacacta aattaccgga
      301 tcaattcggg ggaaaaatgc tttctgaaaa cacgactatt ctgatggcta acggtgaaat
      361 taaagacatc gcaaacgtca cggctaactc ttacgttatg tgcgcagatg gctccgctgc
      421 ccgcgtcata aatgtcacac agggctatca gaaaatctat aatatacagc aaaaaaccaa
      481 acacagagct tttgaaggtg aacctggtag gttagatccc aggcgtagaa cagtttatca
      541 gcgtcttgca ttacaatgta ctgcaggtca taaattgtca gtcagggtcc ctaccaaacc
      601 actgttggaa aaaagtggta gaaatgccac caaatataaa gtgagatgga gaaatctgca
      661 gcaatgtcag acgcttgatg gtaggataat aataattcca aaaaaccatc ataagacatt
      721 cccaatgaca gttgaaggtg agtttgccgc aaaacgcttc atagaagaaa tggagcgctc
      781 taaaggagaa tatttcaact ttgacattga agttagagat ttggattatc ttgatgctca
      841 attgagaatt tctagctgca taagatttgg tccagtactc acaggaaatg gtgttttatc
      901 taaatttctc actggacgta gtgaccttgt aactcctgct gtaaaaagta tggcttggat
      961 gcttggtctg tggttaggtg acggtacaac aaaagagcca gaaatctcag tagatagctt
     1021 ggatcctaag ctaatggaga gtttaagaga aaatgcgaaa atctggggtc tctaccttac
     1081 ggtttgtgac gatcacgttc cgctacgtgc caaacatgta aggcttcatt atggagatgg
     1141 tccagatgaa aacaggaaga caaggaattt gaggaaaaat aatccattct ggaaagctgt
     1201 cacaatttta aagtttaaaa gggatcttga tggagagaag caaatccctg aatttatgta
     1261 cggcgagcat atagaagttc gtgaagcatt cttagccggc ttgatcgact cagatgggta
     1321 cgttgtgaaa aagggcgaag gccctgaatc ttataaaata gcaattcaaa ctgtttattc
     1381 atccattatg gacggaattg tccatatttc aagatctctt ggtatgtcag ctactgtgac
     1441 gaccaggtca gctagggagg aaatcattga aggaagaaaa gtccaatgtc aatttacata
     1501 cgactgtaat gttgctgggg gaacaacttt acagaatgtt ttgtcatatt gtcgaagtgg
     1561 tcacaaaaca agagaagttc cgccaattat aaaaagggaa cccgtatatt tcagcttcac
     1621 ggatgatttc cagggtgaga gtactgtata tgggcttacg atagaaggcc ataaaaattt
     1681 cttgcttggc aacaaaatag aagtgaaatc atgtcgaggc tgctgtgtgg gagaacagca
     1741 taaaatatca caaaaaaaga atctaaaaca ctgtgttgct tgtcccagaa agggaatcaa
     1801 gtatttttat aaagattgga gtggtaaaaa tcgagtatgt gctagatgct atggaagata
     1861 caaattcagc ggtcatcact gtataaattg caagtatgta ccagaagcac gtgaagtgaa
     1921 aaaggcaaaa gacaaaggcg aaaaattggg cattacgccc gaaggtttgc cagttaaagg
     1981 accagagtgt ataaaatgtg gcggaatctt acagtttgat gctgtccgcg ggcctcataa
     2041 gagttgtggt aacaacgcag gtgcgcgcat ctgccggatc cccgggttaa ttaacggtgg
     2101 aggtggaggt ggaggtggag gtgaacaaaa gctaatctcc gaggaagact tgaacggtga
     2161 acaaaaatta atctcagaag aagacttgaa cggactcgac ggtgaacaaa agttgatttc
     2221 tgaagaagat ttgaacggtg aacaaaagct aatctccgag gaagacttga acggtgaaca
     2281 aaaattaatc tcagaagaag acttgaacgg actcgacggt gaacaaaagt tgatttctga
     2341 agaagatttg aacggtgaac aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa
     2401 attaatctca gaagaagact tgaacggact cgacggtgaa caaaagttga tttctgaaga
     2461 agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg aacaaaaatt
     2521 aatctcagaa gaagacttga acggactcga cggtgaacaa aagttgattt ctgaagaaga
     2581 tttgaacggt gaacaaaagc taatctccga ggaagacttg aacggtgaac aaaaattaat
     2641 ctcagaagaa gacttgaacg gactcgacgg tgaacaaaag ttgatttctg aagaagattt
     2701 gaacggtgaa caaaagctaa tctccgagga agacttgaac ggtgaacaaa aattaatcaa
     2761 tcactagtga attcgcgcca cttctaaata agcgaatttc ttgagggccg catcatgtaa
     2821 ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa
     2881 ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
     2941 agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt
     3001 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc
     3061 ggccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3121 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3181 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3241 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3661 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3901 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5221 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     5281 ggcgtatcac gaggcccttt cgtcttcaag aattcagctg aagcttcgta cgctgcaggt
     5341 cgacggatcc ccgggttaat taaggcgcgc cagatctgtt tagcttgcct cgtccccgcc
     5401 gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg
     5461 cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata
     5521 atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc
     5581 gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc
     5641 cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat
     5701 acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc
     5761 atgggtaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     5821 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     5881 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     5941 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     6001 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     6061 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     6121 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     6181 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     6241 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     6301 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     6361 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     6421 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     6481 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     6541 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     6601 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     6661 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     6721 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     6781 aaggaataat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     6841 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     6901 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
     6961 tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc
     7021 cagtgtcgaa aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcaa
     7081 ttctcatgtt tgacagctta tcatcgatcg tccaactgca tggagatgag tcgtggcaag
     7141 aataccaaga gttcctcggt ttgccagtta ttaaaagact cgtatttcca aaagactgca
     7201 acatactact cagtgcagct tcacagaaac ctcattcgtt tattcccttg tttgattcag
     7261 aagcaggtgg gacaggtgaa cttttggatt ggaactcgat ttctgactgg gttggaaggc
     7321 aagagagccc cgagagctta cattttatgt tagctggtgg actgacgcca gaaaatgttg
     7381 gtgatgcgct tagattaaat ggcgttattg gtgttgatgt aagcggaggt gtggagacaa
     7441 atggtgtaaa agactctaac aaaatagcaa atttcgtcaa aaatgctaag aaataggtta
     7501 ttactgagta gtatttattt aagtattgtt tgtgcacttg cctgcaagcc ttttgaaaag
     7561 caagcataaa agatctaaac ataaaatctg taaaataaca agatgtaaag ataatgctaa
     7621 atcatttggc tttttgattg attgtacagg aaaatataca tcgcaggggg ttgactttta
     7681 ccatttcacc gcaatggaat caaacttgtt gaagagaatg ttcacaggcg catacgctac
     7741 aatgacccga ttcttgctag ccttttctcg gtcttgcaaa caaccgccgg cagcttagta
     7801 tataaataca catgtacata cctctctccg tatcctcgta atcattttct tgtatttatc
     7861 gtcttttcgc tgtaaaaact ttatcacact tatctcaaat acacttatta accgctttta
     7921 ctattatctt ctacgctgac agtaatatca aacagtgaca catattaaac acagtggttt
     7981 ctttgcataa acaccatcag cctcaagtcg tcaagtaaag atttcgtgtt catgcagata
     8041 gataacaatc tatatgttga taattagcgt tgcctcatca atgcgagatc cgtttaaccg
     8101 gaccctagtg cacttacccc acgttcggtc cactgtgtgc cgaacatgct ccttcactat
     8161 tttaacatgt ggaattatcg atgccggtgt tattcttgct atgataggcc atcgaatgtt
     8221 aaagggaaga tttattgttt tgccctttaa agacgaattt ggaccgtgaa tgtttaaata
     8281 attctcatgt ttgacagctt atcatcgaac tctaagaggt gatacttatt tactgtaaaa
     8341 ctgtgacgat aaaaccggaa ggaagaataa gaaaactcga actgatctat aatgcctatt
     8401 ttctgtaaag agtttaagct atgaaagcct cggcattttg gccgctccta ggtagtgctt
     8461 tttttccaag gacaaaacag tttctttttc ttgagcaggt tttatgtttc ggtaatcata
     8521 aacaataaat aaattatttc atttatgttt aaaaataaaa aataaaaaag tattttaaat
     8581 ttttaaaaaa gttgattata agcatgtgac cttttgcaag caattaaatt ttgcaatttg
     8641 tgatttaggc aaaagttact atttctggct cgtgtaatat atgtatgcta atgtgaactt
     8701 ttacaaagtc gatatggact tagtcaaaag aaattttctt aaaaatatat agcactagcc
     8761 aatttagcac ttctttatga gatatattat agactttatt aagccagatt tgtgtattat
     8821 atgtatttac ccggcgaatc atggacatac attctgaaat aggtaatatt ctctatggtg
     8881 agacagcata gataacctag gatacaagtt aaaagctagt actgttttgc agtaattttt
     8941 ttctttttta taagaatgtt accacctaaa taagttataa agtcaatagt taagtttgat
     9001 atttgattgt aaaataccgt aatatatttg catgatcaaa aggctcaatg ttgactagcc
     9061 agcatgtcaa ccactatatt gatcaccgat attaggactt ccacaccaac tagtaatatg
     9121 acaataaatt caagatattc ttcatgagaa tggcccagcg attatgcggt gtgaaatacc
     9181 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     9241 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     9301 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     9361 aacgacggcc agtgaattct tattacgatc ctcgcgcccc ctacccaccg tactcgtcaa
     9421 ttccaagggc atcggtaaac atctgctcaa actcgaagtc ggccatatcc agagcgccgt
     9481 agggggcgga gtcgtggggg gtaaatcccg gacccgggga atccccgtcc cccaacatgt
     9541 ccagatcgaa atcgtctagc gcgtcggcat gcgccatcgc cacgtcctcg ccgtctaagt
     9601 ggagctcgtc ccccaggctg acatcggtcg ggggggccgt cgacagtctg cgcgtgtgtc
     9661 ccgcggggag aaaggacagg cgcggagccg ccagccccgc ctcttcgggg gcgtcgtcgt
     9721 ccgggagatc gagcaggccc tcgatggtag acccgtaatt gtttttcgta cgcgcgcggc
     9781 tgtacgcgga cccactttca catttaagtt gtttttctaa tccgcatatg atcaattcaa
     9841 ggccgaataa gaaggctggc tctgcacctt ggcgatcaaa taattcgata gcttgtcgta
     9901 ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg acttgatgct
     9961 cttgttcttc caatacgcaa cctaaagtaa agtgccccac agcgctgagt gcatataatg
    10021 cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg agagtttcat
    10081 actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga tgacttagta
    10141 aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt tccccttcta
    10201 aagggcaaaa gtgagtatgg tgcctatcta acatctcaat gggtaaggcg tcgagcaaag
    10261 cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc ttctgggcga
    10321 gtttacgggt tgttaaacct tcgattccga ccccattaag cagctctaat gcgccgttaa
    10381 tcactttact tttatctaat ctagacatat gaattaattc ggggccgcgg aggctggatc
    10441 ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag gcgatctgac
    10501 ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
    10561 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
    10621 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
    10681 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
    10741 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
    10801 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
    10861 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
    10921 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
    10981 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
    11041 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
    11101 atggcggtaa tgttggacat gagccaatat aaatgtacat attatgatat ggatacaacg
    11161 tatgcaatgg gccaagctcc tcgagtaatt cgcgccactt ctaataagcg atttcttatg
    11221 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
    11281 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
    11341 gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta ccggcagatc
    11401 cgctagggat aacagggtaa tatagatcaa ttcctcgatc gagtttacca ctccctatca
    11461 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
    11521 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
    11581 ctatcagtga tagaga
//
