LOCUS       sequence-268909-       11378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11378)
  AUTHORS   Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso 
            CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, 
            Lindquist S, Khalil AS
  TITLE     A Genetic Tool to Track Protein Aggregates and Control Prion 
            Inheritance.
  JOURNAL   Cell. 2017 Nov 2;171(4):966-979.e18. doi: 
            10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.
  PUBMED    29056345
REFERENCE   2  (bases 1 to 11378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.041"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "966-979.e18"
COMMENT     Sequence Label: pGAN230
FEATURES             Location/Qualifiers
     source          1..11378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             602..622
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             641..1258
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(711..730)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      1993..2179
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2047..2071)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            2254..3373
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        2254..2597
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2598..3170
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      3176..3373
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     3943..3959
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3943..3959
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3973..3992)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3974..3992)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3999..4016)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3999..4015)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4008..4030)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4315..4334)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4447..4468
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4622..4641)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4741..4763
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4801..4819)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4887..4991
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4992..5060,5061..5852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5210..5229)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6023..6611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6512..6531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6765..6782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6899..6920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6975..6991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6980..7002
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6999..7015
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6999..7015
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    8532..8656
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(8681..8686,8687..8704,8705..8711)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8765..9424
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8831..8850)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9766..10071
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9950..9969
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10112..10236)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             10274..10294
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10898..10987
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             10994..11083
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
       61 atttaacgcg aattttaaca aaatattaac gtttacaatt tcctgatgcg gtattttctt
      121 taattaaaga gtgaggactc gaaaatatta atagagtgag gactcgaaaa tattaataga
      181 gtgaggactc gaaaatatta atagagtgag gactcgaaaa tattaatatc gatagagtga
      241 ggactcgaaa atattaatag agtgaggact cgaaaatatt aatgaattca gagtgaggac
      301 tcgaaaatat taatagagtg aggactcgaa aatattaatg gccggcccag atccgccagg
      361 cgtgtatata tagcgtggat ggccaggcaa ctttagtgct gacacataca ggcatatata
      421 tatgtgtgcg acgacacatg atcatatggc atgcatgtgc tctgtatgta tataaaactc
      481 ttgttttctt cttttctcta aatattcttt ccttatacat taggaccttt gcagcataaa
      541 ttactatact tctatagaca cacaaacaca aatacacaca ctgtttaaac aaaaaatggc
      601 tccaaagaag aagagaaagg ttggtattca tggtgttcca tcaagattag ataaaagtaa
      661 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
      721 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
      781 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
      841 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
      901 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
      961 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
     1021 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
     1081 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
     1141 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
     1201 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtctgg
     1261 ttctggtggt tctgaattga tcaaagaaaa catgcacatg aagttgtaca tggaaggtac
     1321 tgttaacaac catcatttca agtgtacctc tgaaggtgaa ggtaaaccat atgaaggtac
     1381 tcaaaccatg agaatcaaag ctgttgaagg tggtccattg ccatttgctt ttgatatttt
     1441 ggctacctcc ttcatgtacg gttctaagac tttcattaac cacacccaag gtatcccaga
     1501 tttcttcaaa caatctttcc cagaaggttt cacttgggaa agagttacta cttacgaaga
     1561 tggtggtgtt ttgactgcta ctcaagatac ttctttacaa gacggttgct tgatctacaa
     1621 cgttaagatt agaggtgtca acttcccatc taatggtcca gttatgcaaa aaaagacttt
     1681 gggttgggaa gcttctactg aaacattata tccagctgat ggtggtttgg aaggtagagc
     1741 tgatatggct ttgaaattgg ttggtggtgg tcatttgatc tgcaacttga aaactaccta
     1801 cagatctaag aagccagcca agaatttgaa aatgccaggt gtttactacg tcgatagaag
     1861 attggaaaga atcaaagaag ccgacaaaga aacctacgtt gaacaacacg aagttgctgt
     1921 tgctagatat tgtgatttgc catccaaatt aggtcataga ggatcttaat agggcgcgcc
     1981 acttctaaat aagcgaattt ctatgattta tgatttttat tattaaataa gttataaaaa
     2041 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct
     2101 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag gtcgctctta
     2161 ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatag atctgtttag
     2221 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
     2281 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
     2341 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
     2401 aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg
     2461 ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg
     2521 aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca
     2581 tccgaacata aacaaccatg ggtaccactc ttgacgacac ggcttaccgg taccgcacca
     2641 gtgtcccggg ggacgccgag gccatcgagg cactggatgg gtccttcacc accgacaccg
     2701 tcttccgcgt caccgccacc ggggacggct tcaccctgcg ggaggtgccg gtggacccgc
     2761 ccctgaccaa ggtgttcccc gacgacgaat cggacgacga atcggacgac ggggaggacg
     2821 gcgacccgga ctcccggacg ttcgtcgcgt acggggacga cggcgacctg gcgggcttcg
     2881 tggtcgtctc gtactccggc tggaaccgcc ggctgaccgt cgaggacatc gaggtcgccc
     2941 cggagcaccg ggggcacggg gtcgggcgcg cgttgatggg gctcgcgacg gagttcgccc
     3001 gcgagcgggg cgccgggcac ctctggctgg aggtcaccaa cgtcaacgca ccggcgatcc
     3061 acgcgtaccg gcggatgggg ttcaccctct gcggcctgga caccgccctg tacgacggca
     3121 ccgcctcgga cggcgagcag gcgctctaca tgagcatgcc ctgcccctaa tcagtactga
     3181 caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt
     3241 tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg
     3301 cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct
     3361 ggtcgctata ctgctgtcga ttcgatacta acgccgccat ccagtgtcga aaacgagctc
     3421 gaattcctgg gggaacaact tcacagaatg ttttgtcata ttgtcgaagt ggtcacaaaa
     3481 caagagaagt tccgccaatt ataaaaaggg aacccgtata tttcagcttc acggatgatt
     3541 tccagggtga gagtactgta tatgggctta cgatagaagg ccataaaaat ttcttgcttg
     3601 gcaacaaaat agaagtgaaa tcatgtcgag gctgctgtgt gggagaacag cataaaatat
     3661 cacaaaaaaa gaatctaaaa cactgtgttg cttgtcccag aaagggaatc aagtattttt
     3721 ataaagattg gagtggtaaa aatcgagtat gtgctagatg ctatggaaga tacaaattca
     3781 gcggtcatca ctgtataaat tgcaagtatg taccagaagc acgtgaagtg aaaaaggcaa
     3841 aagacaaagg cgaaaaattg ggcattacgc ccgaaggttt gccagttaaa ggaccagagt
     3901 gtataaaatg tggcggaatc ttacagtggc ctatgcggcc gctctagaac tagtggatcg
     3961 atccccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
     4021 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4081 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4141 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4201 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     4261 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
     4321 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4381 ggggtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4441 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     4501 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     4561 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     4621 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4681 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4741 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4801 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4861 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4921 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4981 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5041 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5101 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5161 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5221 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5281 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5341 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5401 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5461 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5521 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5581 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5641 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5701 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5761 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5821 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5881 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5941 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6001 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6061 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtat
     6181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6901 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccgcggctcg
     6961 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7021 ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc cagctgaagc
     7081 ttaattatcc tgggcacgag tgaaacaaag ctaaaacctt tatttagcat ggccattgaa
     7141 tgtaacaatt atatatatcg caagcacaaa aaatcaagga gagagaacta ccactttgtt
     7201 catgtgtaca atgttcatta tctccataag caaaaaaaaa aaaatagaaa acatatgcta
     7261 taaggttgat attctcacga gtaagcggca cttgctactt attgacattg cagatttttg
     7321 gctacagaaa tagtatatta gagattataa ttgctaatca aatcaaaata taaaattagt
     7381 aaaccaaacc atttataccc ttccttagta gttatggatt gttttttaat gatatttctg
     7441 caaaccaaag aaagattgtt atccagatag aatttagttt tgatattcat ttttttgttg
     7501 aagattgaac gccatatctg ggcctcataa ttcaaaagac ggtgccatta tcggtagcgt
     7561 ttcgcattgt actggatttc agaaatttca cagttgatga atcgaaaaga atggtctcat
     7621 tgcaacacgt aaggttaaga tgtccctttt taccattata ggcaataaat gaatcataaa
     7681 acgaccgtat actggtgaaa tagtagggag aacgagtacc tgtagtaaaa agtataaatc
     7741 atagttaatc gggcaatgtc cctcgatcaa ggagtattgt gtcatgttcg agacaaacgc
     7801 caacattttt gtttcttttg gacaaatgtt gtttgcattt atgatccgtt atattttgat
     7861 ctaatgtaga gttgcacgta gttcttactg gcaaagaaat cgatgcatac caaaaaagaa
     7921 taaaggtgat atttgatctt taccgtttag ttccaacgta aaattgtgcc tttggactta
     7981 aaatggcgtc gtacgctgca ggtcgacgga tcccaaccac acaaaaatca tacaacgaat
     8041 ggtatatgct tcatttcttt gtttcgcatt agctgcgcta tttgactcaa attattattt
     8101 tttactaaga cgacgcgtca cagtgttcga gtctgtgtca tttcttttgt aattctctta
     8161 aaccacttca taaagttgtg aagttcatag caaaattctt ccgcaaaaag atgaatctta
     8221 gttctcagcc caccaaaaga ggtacatgct aagatcatac agaagttatt gtcacttctt
     8281 accttgctct taaatgtaca ttacaaccgg gtattatatc ttacatcatc gtataatatg
     8341 atctttcttt atggagaaaa tttttttttc actcgaccaa agctcccatt gcttctgaag
     8401 agtgtagtgt atattggtac atcttctctt gaaagactcc attgtactgt aacaaaaagc
     8461 ggtttcttca tcgacttgct cggaataaca tctatatctg cccactagca acaactagtg
     8521 gatcccccat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
     8581 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     8641 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     8701 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     8761 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     8821 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     8881 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     8941 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     9001 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     9061 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     9121 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     9181 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     9241 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     9301 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     9361 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     9421 gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     9481 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     9541 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     9601 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     9661 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     9721 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     9781 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     9841 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     9901 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     9961 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
    10021 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
    10081 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
    10141 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
    10201 ttacgtttct cgttcagctt tcttgtacaa agtggtgatg ggctgcagga attcgatatc
    10261 aagcttaatg gctccaaaga agaagagaaa ggttggtatt catggtgttc cagttgatgg
    10321 tggtttacaa gctccaccaa ctgatgtttc tttgggtgat gaattgcatt tggatggtga
    10381 agatgttgct atggctcatg ctgatgcttt ggatgatttt gatttggata tgttgggtga
    10441 cggtgattct ccaggtccag gttttactcc acatgattct gctccatatg gtgctttaga
    10501 tatggctgac tttgaattcg aacaaatgtt caccgatgcc ttgggtattg atgaatatgg
    10561 tggtggtgct agaccaggtg aaagaccatt tcaatgtaga atctgcatga gaaacttctc
    10621 cagacaagat agattggata gacataccag aactcatact ggtgaaaagc cattccaatg
    10681 cagaatatgt atgagaaatt tttcccaaaa agaacacttg gccggtcatt tgagaacaca
    10741 cacaggtgaa aaaccttttc aatgcagaat atgcatgaga aactttagta gaagagacaa
    10801 cttgaacaga cacttgaaaa cccatttgag aggttctgcc ggcgaattgg ctttgaaatt
    10861 ggctggtttg gatatcaatt ctggttctgc tgctagatac ccatatgatg ttccagatta
    10921 tgctggttac ccttacgatg ttcctgacta cgcaggttct tatccatacg atgtacctga
    10981 ttatgcctca agatatcctt atgacgtccc tgattacgcc ggttatccat atgacgtacc
    11041 agactacgct ggtagttacc catacgacgt cccagattac gcttcttcat aatgactcga
    11101 gtcatgtaat ttcttgcaaa cataagtaaa tgcaaacaca ataataccga tcataaagca
    11161 ttttcttcta tattaaaaaa caaggtttaa taaagctgtt atatatatat atatatatat
    11221 agacgtataa ttagtttagt tctttttgta ccatatacca taaacaaggt aaacttcacc
    11281 tctcaatata tctagaattt cataaaaata tctagcaagg tttcaactcc ttcaatcacg
    11341 ttttcatcac tcaaccctat ctcggtctat tcttttga
//
