LOCUS       sequence-268911-       11073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains Crz1* with CLASP. This consists of the plasma 
            membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP
            modulates nuclear localization of Crz1* in response to blue light..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11073)
  AUTHORS   Chen SY, Osimiri LC, Chevalier M, Bugaj LJ, Nguyen TH, Greenstein 
            RA, Ng AH, Stewart-Ornstein J, Neves LT, El-Samad H
  TITLE     Optogenetic Control Reveals Differential Promoter Interpretation of 
            Transcription Factor Nuclear Translocation Dynamics.
  JOURNAL   Cell Syst. 2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 
            10.1016/j.cels.2020.08.009.
  PUBMED    32898473
REFERENCE   2  (bases 1 to 11073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 
            10.1016/j.cels.2020.08.009."
COMMENT     Sequence Label: pCLASP_Crz1(19A)_CLASP_RGS2membrane
FEATURES             Location/Qualifiers
     source          1..11073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3496..4200
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVATVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3638..3656)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3906..3925
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        4992..5658
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5528..5551
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     promoter        7514..7857
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      8676..8873
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(9396..10211)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9396..10211)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10119..10138
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10203..10222
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10436..11024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10925..10944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggccgcca
       61 atagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa
      121 gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa
      181 cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct
      241 gttgctgcca agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg
      301 gatgttcgta ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt
      361 ttactaaaaa cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg
      421 ctaaaggcat tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac
      481 attggtaata cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca
      541 gacattacga atgcacacgg tgtggtgccc tgaattcgca tctagactga actggccgat
      601 aattgcagac gaacgggcaa ccaaacccat acatcgggat tcctataata ccttcgttct
      661 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      721 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      781 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      841 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      901 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      961 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
     1021 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactccc
     1081 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
     1141 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
     1201 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
     1261 gcatacaata tgggttctgt tgataataag tttaataaag agaaaacaag agctggagcc
     1321 gaaatacact ctttgcctaa cttaaatgta gaacagaagt tcgcttttat agtatctttg
     1381 tttgatgatc catcacaatc tgccaatttg ttggcagagg ctaagaaatt aaacgacgct
     1441 caagcaccaa aaggttcttc attcagcaac ggaaatatgg cttcctacat gactagtagt
     1501 aatggagagg agcaaagtat aaacaacaaa aacgatatag acgataacgc tgcttacaga
     1561 cgtaataatt tcaggaatag tagcaattca ggatcacata cgttccaatt atcggacttg
     1621 gacttagatg tggatatgag gatggattcc gccaatgctg cagagaaaat atcaaagaac
     1681 ctttcctctg gcatcccgga ctcatttgat tctaacgtga acagcttgct gtctccgtca
     1741 agcggttctt attctgcaga tttgaattac caaagtctat acaaaccaga tcttccacag
     1801 caacagttac agcagcaaca gctccaacag cagcagcaac agcagcaaca acagcaacag
     1861 caacaacaga agcaaacacc aactttaaaa gtcgaacaat ctgatacatt tcagtgggac
     1921 gatatcttag ctcctgctga taatcaacat cggccagcac tcgccaacca gtttttagct
     1981 ccaagatcta attacgatgg taccactagg agctcgggca ttgactccaa ttatagcgat
     2041 acagaatcaa actatcatac gccttatttg tatccacagg acttagtttc ttcacctgcg
     2101 atgtctcact taaccgcgaa taacgatgat tttgacgatc ttttgagcgt cgcatctatg
     2161 aactcaaatt atttactgcc cgtaaatgct catggttata aacatatttc aaaccttgat
     2221 gagttggatg acttgctatc tttaacatat tcagacaata accttttatc agcatcaaac
     2281 aacagtgatt tcaacaacag taataacgga attattaata ccgctgacac tcaaaacagt
     2341 accattgcca ttaataaaag taaagtcggt actaaccaaa agatgttatt gactattcca
     2401 acttcttcca cacctgcccc ttccactcat gctgccccgg taacacccat catttctata
     2461 caggaattca atgagggaca cttcccagtt aaaaacgaag atgacggaac gttacaacta
     2521 aaagttcggg ataacgaagc ctatagcgct actaacaata acaatctact tcgtccagat
     2581 gataatgatt acaacaacga agctctcgct gatattgacc gctccttcga agatatcatt
     2641 aacggtcgaa aattgaaact taaaaaatca agaagaagag cagcccaagc tgcaaataat
     2701 agtttcacta gcaggagatc cgctagagcc agagctatag cacccgatga aaaagctaaa
     2761 tctataagtg caaataggga aaagttattg gagatggcag atcttttacc atccagtgaa
     2821 aatgacaata atcgggagcg ctatgacaat gatagcaaaa ctagctataa caccattaac
     2881 agtagcaatt ttaatgagga taataataat aacaatttgt taacaagtaa accaaaaatt
     2941 gaatcgggca ttgtcaatat taaaaatgaa ctagatgaca ctagcaaaga tcttggcata
     3001 cttctagaca tagatagctt gggccaattt gagcagaaag ttggtttcaa aaatgatgat
     3061 aatcacgaaa ataacgataa tggcacattt tctgttaaga aaaatgacaa tctagaaaaa
     3121 ctagacagtg taacaaataa taggaaaaat cctgcgaatt ttgcttgtga tgtatgtggt
     3181 aagaagttta caagacctta taacttaaag tcacacttaa gaacgcatac aaatgaaagg
     3241 ccatttattt gttctatttg tggtaaggcg tttgcacgtc aacatgatag aaagagacac
     3301 gaagatttgc atacaggtaa gaaaaggtat gtatgcggtg gtaagctgaa ggacggtaaa
     3361 ccctggggtt gtggcaaaaa gtttgcaaga agtgatgctc ttggtaggca ttttaaaact
     3421 gaaagtggta gaagatgcat cactcccttg tacgaagaag ccagacagga gaaatcggga
     3481 caagagagtg gatccgtgag caagggcgag gaggataaca tggccatcat caaggagttc
     3541 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     3601 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     3661 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     3721 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     3781 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg cgaccgtgac ccaggactcc
     3841 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3901 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     3961 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     4021 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     4081 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     4141 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     4201 ggatcccccg aattcggtat tgatctgagc ggcctgaccc tgcagggttc tggaagtggg
     4261 tcgttggcta ctacacttga acgtattgag aagaactttg tcattactga cccaagattg
     4321 ccagataatc ccattatatt cgcgtccgat agtttcttgc agttgacaga atatagccgt
     4381 gaagaaattt tgggaagaaa ctgcaggttt ctacaaggtc ctgaaactga tcgcgcgaca
     4441 gtgagaaaaa ttagagatgc catagataac caaacagagg tcactgttca gctgattaat
     4501 tatacaaaga gtggtaaaaa gttctggaac ctctttcact tgcagcctat gcgagatcag
     4561 aagggagatg tccagtactt tattggggtt cagttggatg gaactgagca tgtccgagat
     4621 gctgccgaga gagagggagt catgctgatt aagaaaactg cagaaaatat tgatgctgct
     4681 gctaagagat cttggtctat ggctttcgag ctctaatggc attgaattga attgaaatcg
     4741 atagatcaat ttttttcttt tctctttccc catcctttac gctaaaataa tagtttattt
     4801 tattttttga atatttttta tttatatacg tatatataga ctattattta tcttttaatg
     4861 attattaaga tttttattaa aaaaaaattc gctcctcttt taatgccttt atgcagtttt
     4921 tttttcccat tcgatatttc tatgtgctgc caaaccagat gtcaacacag ctacaacgca
     4981 gttcgagttt atcattatca atactgccat ttcaaagaat acgtaaataa ttaatagtag
     5041 tgattttcct aactttattt agtcaaaaaa ttagcctttt aattctgctg taacccgtac
     5101 atgcccaaaa tagggggcgg gttacacaga atatataaca tcgtaggtgt ctgggtgaac
     5161 agtttattcc tggcatccac taaatataat ggagcccgct ttttaagctg gcatccagaa
     5221 aaaaaaagaa tcccagcacc aaaatattgt tttcttcacc aaccatcagt tcataggtcc
     5281 attctcttag cgcaactaca gagaacaggg gcacaaacag gcaaaaaacg ggcacaacct
     5341 caatggagtg atgcaacctg cctggagtaa atgatgacac aaggcaattg acccacgcat
     5401 gtatctatct cattttctta caccttctat taccttctgc tctctctgat ttggaaaaag
     5461 ctgaaaaaaa aggttgaaac cagttccctg aaattattcc cctacttgac taataagtat
     5521 ataaagacgg taggtattga ttgtaattct gtaaatctat ttcttaaact tcttaaattc
     5581 tacttttata gttagtcttt tttttagttt taaaacacca agaacttagt ttcgaataaa
     5641 cacacataaa caaacaaaag atctatgaag agaacattgt tgaaggattg gaaaaccaga
     5701 ttatcatact tcttacagaa ctcatctaca cctggtaagc caaagacagg taagaaatct
     5761 aaacaacaag gcggtggcgg aagtggcgga ggaggttcgg gcggaagtgg ttctgctgaa
     5821 ggttctgttg ctagacaacc agacttgttg acttgtgacg acgaaccaat ccacatccca
     5881 ggtgctatcc aaccacacgg tttgttgttg gctttggctg ctgacatgac tatcgttgct
     5941 ggttctgaca acttgccaga attgactggt ttggctatcg gtgctttgat cggtagatct
     6001 gctgctgacg ttttcgactc tgaaactcac aacagattga ctatcgcttt ggctgaacca
     6061 ggtgctgctg ttggtgctcc aatcactgtt ggtttcacta tgagaaagga cgctggtttc
     6121 atcggttctt ggcacagaca cgaccaattg atcttcttgg aattggaacc accacaaaga
     6181 gatgttgctg aaccacaagc tttcttcaga agaactaact ctgctatcag aagattgcaa
     6241 gctgctgaaa ctttggaatc tgcttgtgct gctgctgctc aagaagttag aaagatcact
     6301 ggtttcgaca gagttatgat ctacagattc gcttctgact tctctggtga agttatcgct
     6361 gaagacagat gtgctgaagt tgaatctaag ttgggtttgc actacccagc ttctactgtt
     6421 ccagctcaag ctagaagatt gtacactatc aacccagtta gaatcatccc agacatcaac
     6481 tacagaccag ttccagttac tccagacttg aacccagtta ctggtagacc aatcgacttg
     6541 tctttcgcta tcttgagatc tgtttctcca gttcacttgg aattcatgag aaacatcggt
     6601 atgcacggta ctatgtctat ctctatcttg agaggtgaaa gattgtgggg tttgatcgtt
     6661 tgtcaccaca gaactccata ctacgttgac ttggacggta gacaagcttg tgaattggtt
     6721 gctcaagttt tggcttggca aatcggtgtt atggaagaag gatccttagc tactacgcta
     6781 gagcgtatag aaaaaaattt cgtgatcacc gatccaagat tgccggacaa cccaatcata
     6841 tttgcttccg attccttttt acaactgacc gaatacagta gagaggagat tctgggtagg
     6901 aattgcaggt tccttcaggg tcccgagact gatcgtgcaa cagttaggaa aattagagat
     6961 gcgatagaca gccagacaga agttaccgtg cagttgatca attataccaa gagcggaaaa
     7021 aaattctgga acttatttca tttacagcca atgcgtgacc agaagggcga cgtacagtat
     7081 ttcattggtg tgcaactaga cgggacagag cacgtacgtg acgcagcaga aagagagggc
     7141 gtgatgttga taaaaaaaac tgcagagaat atcgacgagg ctgcgaaaga gctataatgg
     7201 cattgaattg aattgaaatc gatagatcaa tttttttctt ttctctttcc ccatccttta
     7261 cgctaaaata atagtttatt ttattttttg aatatttttt atttatatac gtatatatag
     7321 actattattt atcttttaat gattattaag atttttatta aaaaaaaatt cgctcctctt
     7381 ttaatgcctt tatgcagttt ttttttccca ttcgatattt ctatgtgctg agcagttaca
     7441 gagatgttac gaaccactag tgcactgcag tacagtttag cttgcctcgt ccccgccggg
     7501 tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag
     7561 ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc
     7621 atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct
     7681 gcggacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc
     7741 gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact
     7801 tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg
     7861 acagtcaaca ctaagaccta tagtgagaga gcagaaactc atgcctcacc agtagcacaa
     7921 cgattatttc gattaatgga actgaagaaa accaatttat gtgcatcaat tgatgttgat
     7981 accactaagg aattccttga attaattgat aaattgggtc cttatgtatg cttaatcaag
     8041 actcatattg atataatcaa tgatttttcc tatgaatcca ctattgaacc attattagaa
     8101 ctttcacgca aacatcaatt tatgattttt gaagatagaa aatttgctga tattggtaat
     8161 accgtgaaga aacaatatat tggtggagtt tataaaatta gtagttgggc agatattact
     8221 aatgctcatg gtgtcactgg gaatggagta gttgaaggat taaaacaggg agctaaagaa
     8281 accaccacca accaagagcc aagagggtta ttgatgttag ctgaattatc atcagtggga
     8341 tcattagcat atggagaata ttctcaaaaa actgttgaaa ttgctaaatc cgataaggaa
     8401 tttgttattg gatttattgc ccaacgtgat atgggtggac aagaagaagg atttgattgg
     8461 cttattatga cacctggagt tggattagat gataaaggtg atggattagg acaacaatat
     8521 agaactgttg atgaagttgt tagcactgga actgatatta tcattgttgg tagaggattg
     8581 tttggtaaag gaagagatcc agatattgaa ggtaaaaggt atagagatgc tggttggaat
     8641 gcttatttga aaaagactgg ccaattataa tcagtactga caataaaaag attcttgttt
     8701 tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg
     8761 ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg
     8821 cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga
     8881 ttcgatacta acgccgccat ccagtgtcga aaacgacctc gaattcatcg agtggattcg
     8941 gctttgggta ccgggcccga gtggcccagg tattgttagc ggtttgaagc aggcggcaga
     9001 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc
     9061 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt
     9121 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt
     9181 gattatgaca cccggtgtgg gtttagatga caagggagat gcattgggtc aacagtatag
     9241 aaccgtggat gatgtggttt ctacaggatc tgacattatt attgttggaa gaggactatt
     9301 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggccga
     9361 gcggccgcgt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
     9421 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     9481 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     9541 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     9601 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
     9661 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     9721 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgaggcg aaatacgcga
     9781 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     9841 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     9901 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     9961 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
    10021 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
    10081 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
    10141 tataaatcag catccatgtt ggaatttaat cgcggcctgg agcaagacgt ttcccgttga
    10201 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
    10261 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
    10321 tgttgaataa atcgaacttt tgctgagttg aaggatcagt catgaccaaa atcccttaac
    10381 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10441 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10501 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10561 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10621 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10681 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10741 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10801 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10861 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10921 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10981 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11041 cctttttacg gttcctggcc ttttgctggc ctt
//
