LOCUS       sequence-268917-        9087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses full-length human KIF1A fused with mScarlet and StrepII 
            tag in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Chiba K, Takahashi H, Chen M, Obinata H, Arai S, Hashimoto K, Oda T,
            McKenney RJ, Niwa S
  TITLE     Disease-associated mutations hyperactivate KIF1A motility and 
            anterograde axonal transport of synaptic vesicle precursors.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 27. pii: 1905690116. doi: 
            10.1073/pnas.1905690116.
  PUBMED    31455732
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 27. pii: 1905690116. doi: 
            10.1073/pnas.1905690116."
COMMENT     Sequence Label: MOM603
FEATURES             Location/Qualifiers
     source          1..9087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        77..168
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     93..110
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5682..5705
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5706..5729
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5745..6440
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(5878..5896)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6146..6165
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6447..6470
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    6610..6744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6647..6666)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6701..6720
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6865..7030
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      7220..7808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7709..7728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  7943..8166
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8233..8766)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(8955..8960,8961..8977,8978..8983))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(9054..9087)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgtaactat aacggtccta aggtagcgag tttaaacact agtatcgatt cgcgacctac
       61 tccggaatat taatagatca tggagataat taaaatgata accatctcgc aaataaataa
      121 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat
      181 tcataccgtc ccaccatcgg gcgcggatcc cggtccgaag cgcgcggaat tcaaaggcct
      241 acgtcgacga gctcacttgt cgcggccgct ttcgaatcta gagcctgcag accatggccg
      301 gggcttcggt gaaggtggcg gtgcgggtcc gccccttcaa ttcccgggaa atgagccgtg
      361 actccaagtg catcattcag atgtctggaa gcaccaccac cattgttaac cccaaacagc
      421 ccaaggagac gcccaaaagc ttcagctttg actactccta ctggtcgcac acctcacctg
      481 aggacatcaa ctacgcgtcg cagaagcagg tgtaccggga catcggcgag gagatgctgc
      541 agcatgcctt tgagggatac aacgtgtgca tcttcgccta tgggcagacg ggtgccggca
      601 agtcctacac catgatgggc aagcaggaga aggaccagca gggcatcatc ccacagctct
      661 gcgaggacct cttctctcgg atcaacgaca cgaccaacga caacatgtcc tactccgtgg
      721 aggtcagcta catggagatt tactgtgagc gcgtccgtga cctcctgaac cccaagaaca
      781 agggcaacct tcgcgtgagg gagcacccac tgctggggcc ctacgtggag gacctctcca
      841 agctggctgt cacctcctac aatgacatcc aggacctcat ggactcaggg aacaaggcca
      901 ggaccgtggc ggccaccaac atgaatgaga ccagcagtcg ctcccacgcc gtcttcaaca
      961 tcatcttcac ccagaagcgc catgacgcag agaccaatat caccacggag aaggtgagca
     1021 aaatcagcct ggtggacctg gctgggagcg agcgggctga ctccacggga gccaagggca
     1081 cgcgcctcaa ggagggggcc aacatcaaca agtcgctgac caccctgggc aaggtcatct
     1141 ccgccctggc tgaaatggac tccggaccca acaagaacaa gaaaaagaag aagacagatt
     1201 tcattccgta ccgagattcc gtgttgacct ggctcctccg ggaaaacctg ggcggtaact
     1261 caaggacagc tatggtggca gccttgagtc ctgcagacat caactacgat gagaccctta
     1321 gcacgctgag gtatgctgac cgggccaagc agatccgctg caatgctgtc atcaatgagg
     1381 accccaacaa caagctgatc cgcgagctga aggatgaggt gacccggctg cgggaccttc
     1441 tgtacgccca gggtcttggc gacatcactg acaccaacac tgtgcctgga ggacccaaat
     1501 tgaccaatgc cctggtgggt atgagcccct catcctcgct ctcagccctg tccagccgcg
     1561 cggcctccgt gtccagcctc cacgagcgca tcttgtttgc cccgggcagc gaggaggcca
     1621 ttgaaagact gaaggaaaca gagaagatca tagctgagct caatgagacc tgggaggaga
     1681 agctgcggcg gacagaagcc atccggatgg agagggaagc cctgctggcc gagatgggtg
     1741 tggccatgag ggaggatggc ggcaccttgg gcgtattctc tcccaaaaag acaccacacc
     1801 tcgtcaacct gaacgaggac ccgctgatgt ctgagtgcct gctctactac atcaaggatg
     1861 ggatcaccag agtgggcagg gaggatggcg agaggcggca ggacattgtt ctgagtgggc
     1921 acttcatcaa ggaggagcac tgcgtcttcc ggagcgactc caggggaggc agcgaagctg
     1981 tggtgacctt ggagccctgt gagggggcag acacctacgt caatggcaag aaagtcacag
     2041 agcccagcat cctgcgttca ggaaaccgca tcatcatggg taagagccat gtgttccggt
     2101 tcaaccaccc cgagcaggcc cggcaggagc gtgagcgcac gccttgtgcg gagacgccag
     2161 ctgagcctgt ggactgggcc ttcgcccagc gtgagctgct ggagaagcag ggcatcgaca
     2221 tgaagcagga gatggagcag aggctccagg aactggagga ccagtaccgc cgcgagcggg
     2281 aggaggccac ctacctgctg gagcagcagc ggctggacta tgagagcaag ctggaggctc
     2341 tgcagaagca gatggactcc aggtactacc cggaggtgaa cgaggaggag gaggagcccg
     2401 aggatgaagt ccagtggaca gagcgggagt gtgagctggc gctctgggcc ttccggaagt
     2461 ggaagtggta ccagttcacg tctctgcggg acctgctgtg gggcaacgcc atcttcctca
     2521 aggaggccaa tgccatcagc gtggagctga aaaagaaggt acaattccag tttgtcctcc
     2581 tgacggacac actctactcc cctctgccac ccgacctgct gcccccagag gccgccaaag
     2641 accgagagac gcggcccttc ccccgcacca ttgtggccgt ggaggtccag gaccagaaga
     2701 acggggccac ccactactgg acgctggaga agctcaggca gcgtctggac ctgatgcggg
     2761 agatgtacga ccgcgctgca gaggtgccct ccagtgtcat cgaggactgt gacaacgtgg
     2821 tgaccggcgg agaccccttc tatgaccgct tcccctggtt ccggctggtg ggcagttcag
     2881 ccatctctgg ctgcaacagc taccctcttc tcaacacatg catgagcgag cgcatggctg
     2941 ctctcacccc ctcccccacc ttctcgagcc ccgactccga cgccaccgag cctgccgagg
     3001 agcagagcgt gggggaggag gaggaggagg aggaggagga ggaggatgag gaggaggagg
     3061 acctggagga cgacgtcttt ccggagcacg cgctgtgcga cggccgggac ccgttttacg
     3121 accggccccc cctgttcagt ttagtaggaa gggccttcgt gtacctgagc aacctgctgt
     3181 accccgttcc cctggtacac cgtgtggcaa tcgtcagcga gaagggcgag gtgaagggct
     3241 tcctccgcgt ggccgtccag gccatctcag ccgatgaaga ggcccctgat tatggctctg
     3301 gcgtccgcca gtcgggaact gctaaaatct cctttgatga ccagcatttt gaaaagttcc
     3361 agtccgagtc ttgccccgtg gtggggatgt cccgctcggg aacctcccag gaagagcttc
     3421 gcatcgtgga gggccagggc cagggtgcag acgtggggcc ctcagccgat gaagtcaaca
     3481 acaacacctg ttcagcagtg cccccagaag gcctcctcct agacagctct gagaaagccg
     3541 ccctggatgg gcccctggat gctgccctgg accacctccg cctgggcaac accttcacct
     3601 tccgtgtgac agtcctgcag gcgtccagca tctctgccga atatgccgac atcttctgcc
     3661 agttcaactt catccaccgc cacgacgagg ccttctccac agagcccctg aagaacacag
     3721 gcagaggccc cccacttggc ttctaccacg tccagaacat cgcagtggag gtgaccaagt
     3781 ccttcattga gtacatcaag agccagccca ttgttttcga ggtctttggc cactaccagc
     3841 agcacccgtt cccgcccctc tgcaaggacg tgctcagccc cctgaggccc tcgcgccgcc
     3901 acttccctcg ggtcatgcta ctgtccaagc cagtgcccgc caccaagctc agcacactga
     3961 cgcggccctg tccgggaccc tgccactgca agtacgacct gctggtctac ttcgagatct
     4021 gtgagctgga ggccaacggc gattacatcc cggccgtggt ggaccaccgt gggggcatgc
     4081 catgcatggg gaccttcctc ctccaccagg gcatccagcg acggattacg gtgacactac
     4141 tgcatgagac aggcagccat atccgctgga aggaagtgcg cgagctggtc gtgggccgca
     4201 tccgaaacac tccagagacc gacgagtccc tgatcgaccc caacatcttg tctctcaaca
     4261 tcctctcttc cggatacatc cacccagccc aagatgaccg gaccttttac caatttgagg
     4321 ctgcgtggga cagctccatg cacaactctc tcctgctgaa ccgggtcacc ccttatcgag
     4381 agaaaatcta catgacactc tccgcttata tcgagatgga gaactgcacc cagccggctg
     4441 ttgtcaccaa ggacttctgc atggtcttct attcccgtga tgccaagctg ccagcctcgc
     4501 gctccatccg caacctcttt ggcagtggga gccttcgggc ctcagagagt aaccgtgtga
     4561 ctggtgtgta cgagctcagc ctgtgccacg tggctgacgc gggcagccca gggatgcagc
     4621 gccggcgccg acgagtcctg gacacatctg tggcctatgt ccggggcgag gagaacctgg
     4681 caggctggag gccccggagt gacagtctca ttctggacca ccagtgggag ctggagaagc
     4741 tgagcctcct gcaggaggtg gagaagacta ggcactacct gctcctgcgg gagaagctgg
     4801 agaccgccca gcggcctgtc ccggaggcac tgtccccggc cttcagcgag gactctgagt
     4861 cccatggctc ctccagcgcc tcctccccgc tctcggctga gggccgccca tcacccctgg
     4921 aggctcccaa cgagaggcag cgggagctgg ccgtcaagtg cttgcgcctg ctcacgcaca
     4981 cattcaacag agagtacaca cacagccacg tctgcgtcag tgccagcgag agcaagctct
     5041 ccgagatgtc tgtcaccctg ctccgggacc cgtcgatgtc ccctctaggg gtggccactc
     5101 tcaccccctc ctccacttgc ccctctctgg ttgaagggcg gtacggtgcc actgacctga
     5161 ggaccccgca gccctgctcc cggccagcca gcccagagcc cgagctgctg ccagaggccg
     5221 actccaagaa gctcccttcc cctgcccggg caacagagac agacaaggag ccccagcgcc
     5281 tgctggtccc tgacatccag gagatccgag tcagcccgat cgtttccaag aaggggtacc
     5341 tgcacttcct ggagccgcac acgtcaggct gggccaggcg cttcgtggtg gtgcggcgcc
     5401 cctatgccta catgtacaac agcgacaagg acaccgtgga gcggttcgtg ctcaacctgg
     5461 ccactgccca ggtggagtac agtgaggacc agcaggctat gctcaagaca cccaacacat
     5521 tcgcggtgtg cacggaacac cgcggcatcc tgctgcaggc cgccagcgac aaggacatgc
     5581 atgactggct gtacgccttc aaccccctcc tggccgggac catacggtcc aagctctcca
     5641 gaaggaggtc tgcccagatg cgggtcggca gtggatcggg tcttgaggtt ctcttccaag
     5701 gacctctcga agtcctgttt cagggtccag gaagcgggtc tggaatggtg agcaagggcg
     5761 aggcagtgat caaggagttc atgcggttca aggtgcacat ggagggctcc atgaacggcc
     5821 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     5881 agctgaaggt gaccaagggt ggccccctgc ccttctcctg ggacatcctg tcccctcagt
     5941 tcatgtacgg ctccagggcc ttcaccaagc accccgccga catccccgac tactataagc
     6001 agtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg
     6061 tgaccgtgac ccaggacacc tccctggagg acggcaccct gatctacaag gtgaagctcc
     6121 gcggcaccaa cttccctcct gacggccccg taatgcagaa gaagacaatg ggctgggaag
     6181 cgtccaccga gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt aagatggccc
     6241 tgcgcctgaa ggacggcggc cgctacctgg cggacttcaa gaccacctac aaggccaaga
     6301 agcccgtgca gatgcccggc gcctacaacg tcgaccgcaa gttggacatc acctcccaca
     6361 acgaggacta caccgtggtg gaacagtacg aacgctccga gggccgccac tccaccggcg
     6421 gcatggacga gctgtacaag ctcgagtgga gtcatccaca atttgagaag taatctcgac
     6481 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
     6541 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     6601 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6661 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6721 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc cggctgctaa
     6781 caaagcccga aaggaagctg agttggctgc tgccaccgct tagcaataac tatcataacc
     6841 cctagggtat acccatctaa ttggaaccag ataagtgaaa tctagttcca aactattttg
     6901 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     6961 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     7021 tccgcccaca accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt
     7081 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7141 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7801 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7861 ctcacatgtt ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc
     7921 gcagatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag
     7981 atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc
     8041 cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt
     8101 tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact
     8161 atattcgcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc
     8221 ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg
     8281 tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta
     8341 gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc
     8401 aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg
     8461 cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc
     8521 gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc
     8581 cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc
     8641 agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact
     8701 tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg
     8761 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     8821 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
     8881 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     8941 gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca
     9001 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt
     9061 cgtatagcat acattatacg aagttat
//
