LOCUS       sequence-268919-        5877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding Marburg virus VP30 gene under 
            control of the AG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Manhart WA, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Muhlberger 
            E
  TITLE     A Chimeric Lloviu Virus Minigenome System Reveals that the 
            Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than
            Marburgviruses.
  JOURNAL   Cell Rep. 2018 Sep 4;24(10):2573-2580.e4. doi: 
            10.1016/j.celrep.2018.08.008.
  PUBMED    30184492
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.08"; journalName: "Cell Rep"; date: 
            "2018-09-4- 4"; volume: "24"; issue: "10"; pages: "2573-2580.e4"
COMMENT     Sequence Label: pCAGGS_VP30_MARV
FEATURES             Location/Qualifiers
     source          1..5877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          115..1131
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1055..1077
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1139..1158
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2397..2416)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2462..2517
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2516..2535)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2878..2894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2878..2894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2891..2913)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2902..2918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2926..2932,2933..2950,2951..2956))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2971..2992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3051..3246
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3097..3232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3252..3386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3289..3308)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3343..3362
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3454..3471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3625..4213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3705..3724)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4384..5175,5176..5244))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5007..5026
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5245..5349)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5380..5759
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
       61 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
      121 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
      181 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
      241 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
      301 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
      361 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
      421 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
      481 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
      541 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
      601 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
      661 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
      721 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
      781 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
      841 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
      901 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
      961 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     1021 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     1081 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     1141 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcctcga gggtaccccc
     1201 ggggagctca ctagtggatc caaaatgcaa caacctcgcg gaagaagtcg aacccgcaac
     1261 caccaagtca caccgactat atatcatgaa actcaattgc cctccaaacc tcattatacc
     1321 aattatcatc cacgtgcaag atcgatgagc tcaacccgta gtagtgcaga aagtagtccc
     1381 accaatcata ttccccgtgc tcgaccaccc tcaacattca acttatcgaa accccctcct
     1441 cctccaaaag acatgtgcag gaacatgaaa attggattgc cgtgcgctga tcccacttgt
     1501 aatagagatc atgaccttga taatctaaca aatcgtgaac ttttgctatt gatggcccga
     1561 aaaatgctcc ccaatacaga caagaccttt agaagtccgc aggactgtgg atcaccgtct
     1621 ctttctaaag gtctctcaaa agataaacag gagcaaacga aagatgtgtt gaccttggaa
     1681 aatctaggac acattctgag ctatctccac agatcagaaa ttgggaaatt ggatgagaca
     1741 tcacttcgtg cagcattaag tctgacgtgt gctggaattc gaaagacgaa tagatccttg
     1801 atcaacacca tgacagaatt acacatgaac catgaaaatc tcccgcaaga ccaaaacggt
     1861 gttatcaagc agacctatac aggtattcac cttgacaaag gaggtcaatt cgaagccgcc
     1921 ttatggcaag gttgggataa gagatcgata tctctattcg tacaagcagc tttatatgta
     1981 atgaacaata tcccctgtga atcatcaatc agtgtgcaag cctcatacga tcattttatt
     2041 cttcctcaaa gtcaaggtaa aggacagtga ttattgttcg aaagttgaca atttgatcac
     2101 tttcagtttt cagtttcaac ccttatcgcg agacttgaat acaatcctac taacttcaat
     2161 aagtgacccc aaattcaagt ttgctgaaag ctaagatgac aatgatcact agttcattgt
     2221 aaattactcg atcaaaatgt tcttaagcta tcttaagctt actgatgcgg ctctgcttca
     2281 cttttctttt gattttaaag ccatagctat atctaagtgt ctaattaaca acttgtacct
     2341 ctggatccct gcagctcgag aggggggcgg ccgcgagctc ttaattagct agcagatctt
     2401 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     2461 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     2521 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     2581 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     2641 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     2701 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     2761 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     2821 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     2881 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2941 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3001 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     3061 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3121 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3181 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3241 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3301 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3361 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     3421 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3481 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3541 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3601 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3661 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3721 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3781 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3841 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3901 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3961 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4021 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4081 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4141 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4201 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4261 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4321 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4381 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4441 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4501 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4561 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4621 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4681 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4741 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4801 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4861 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4921 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4981 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5041 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5101 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5161 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5221 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5281 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5341 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt
     5401 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     5461 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     5521 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     5581 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     5641 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     5701 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     5761 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     5821 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg ggggggg
//
