LOCUS       sequence-268957-        9242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-fused TAZ by lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Bao Y, Nakagawa K, Yang Z, Ikeda M, Withanage K, Ishigami-Yuasa M, 
            Okuno Y, Hata S, Nishina H, Hata Y
  TITLE     A cell-based assay to screen stimulators of the Hippo pathway 
            reveals the inhibitory effect of dobutamine on the YAP-dependent 
            gene transcription.
  JOURNAL   J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 
            2011 May 17.
  PUBMED    21586534
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/jb/mvr063";
            journalName: "J Biochem"; date: "2011-08"; volume: "150"; issue: 
            "2"; pages: "199-208"
COMMENT     Sequence Label: pLL3.7 EGFPC2 TAZ
FEATURES             Location/Qualifiers
     source          1..9242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2712..3015
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3016..3219
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3258..3267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3264..3266,3267..3269,3270..3980)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3264..3266,3267..3269,3270..3980)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3309..3330)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3570..3589)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3917..3938
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5893..6014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5904..5923)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5958..5977
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(6042..6075)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6131..6719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6184..6204)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6602..6613)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6722..6738)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6723..6739)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7248..7428
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7490..8078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7570..7589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8249..9040,9041..9109))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8872..8891
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9110..9214)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctaggg ccgcgtggat aaccgtatta
     2641 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat
     2701 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     2761 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     2821 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2881 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2941 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3001 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     3061 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     3121 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     3181 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
     3241 cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     3301 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3361 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3421 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3481 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3541 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3601 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3661 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3721 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3781 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3841 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3901 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3961 gcatggacga gctgtacaag tccggccgga ctcagatctc gagctcaagc ttcgaattca
     4021 cgcgctcagg ctcagcttcg ctgcccgccc agaagatgaa tccggcctcg gcgccccctc
     4081 cgctcccgcc gcctgggcag caagtgatcc acgtcacgca ggacctagac acagacctcg
     4141 aagccctctt caactctgtc atgaatccga agcctagctc gtggcggaag aagatcctgc
     4201 cggagtcttt ctttaaggag cctgattcgg gctcgcactc gcgccagtcc agcaccgact
     4261 cgtcgggcgg ccacccgggg cctcgactgg ctgggggtgc ccagcatgtc cgctcgcact
     4321 cgtcgcccgc gtccctgcag ctgggcaccg gcgcgggtgc tgcgggtagc cccgcgcagc
     4381 agcacgcgca cctccgccag cagtcctacg acgtgaccga cgagctgcca ctgcccccgg
     4441 gctgggagat gaccttcacg gccactggcc agaggtactt cctcaatcac atagaaaaaa
     4501 tcaccacatg gcaagaccct aggaaggcga tgaatcagcc tctgaatcat atgaacctcc
     4561 accctgccgt cagttccaca ccagtgcctc agaggtccat ggcagtatcc cagccaaatc
     4621 tcgtgatgaa tcaccaacac cagcagcaga tggcccccag taccctgagc cagcagaacc
     4681 accccactca gaacccaccc gcagggctca tgagtatgcc caatgcgctg accactcagc
     4741 agcagcagca gcagaaactg cggcttcaga gaatccagat ggagagagaa aggattcgaa
     4801 tgcgccaaga ggagctcatg aggcaggaag ctgccctctg tcgacagctc cccatggaag
     4861 ctgagactct tgccccagtt caggctgctg tcaacccacc cacgatgacc ccagacatga
     4921 gatccatcac taataatagc tcagatcctt tcctcaatgg agggccatat cattcgaggg
     4981 agcagagcac tgacagtggc ctggggttag ggtgctacag tgtccccaca actccggagg
     5041 acttcctcag caatgtggat gagatggata caggagaaaa cgcaggacaa acacccatga
     5101 acatcaatcc ccaacagacc cgtttccctg atttccttga ctgtcttcca ggaacaaacg
     5161 ttgacttagg aactttggaa tctgaagacc tgatccccct cttcaatgat gtagagtctg
     5221 ctctgaacaa aagtgagccc tttctaacct ggctgtaatc actaccattg taacttggat
     5281 gtagccatga ccttacattt cctgggcctc ttggaaaaag tgatggagca gagcaagtct
     5341 gcaggtgcac cacttcccgc ctccatgact cgtgctccct cctttttatg ttgccagttt
     5401 aatcattgcc tggttttgat tgagagtaac ttaagttaaa cataaataaa tattctattt
     5461 tcattttctg caagcctgcg ttcttgtgac agattataca gaattgtgtc tgcaggattg
     5521 attatgcaga atacttttct ctttcttctc tgctgcccca tggctaagct ttatgggtgt
     5581 taattgaaat ttatacacca attgatttta aaccataaaa agctgaccac aggcagttac
     5641 ttctgagggc atcttggtcc aggaaatgtg cacaaaattc gacctgattt acagtttcaa
     5701 aaactgtatt gatgacagta gtaccaaatg ctttaaaaac tatttaactt gagctttaaa
     5761 aatcattgta tggatagtaa aattctactg tatggaatac aatgtaattt tgaatccaaa
     5821 aaaaaaaaaa aaaaaactcg acccgggcgg ccgcttccct ttagtgaggg ttaatgcttc
     5881 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     5941 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     6001 gcaataaaca agttaacaac aacaattcgt cgagggacct aataacttcg tatagcatac
     6061 attatacgaa gttatacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa
     6121 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6181 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6241 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6301 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6361 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6421 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6481 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6541 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6601 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6661 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     6721 tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata
     6781 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     6841 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     6901 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     6961 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     7021 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     7081 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     7141 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     7201 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     7261 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7321 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7381 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa
     7441 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7501 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7561 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7621 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7681 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7741 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7801 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7861 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7921 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7981 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8041 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8101 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8161 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8221 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8281 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8341 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8401 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8461 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8521 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8581 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8641 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8701 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8761 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8821 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8881 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8941 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9001 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9061 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9121 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9181 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9241 ac
//
