LOCUS       sequence-268967-        9882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into 
            MYH11 allele.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9882)
  AUTHORS   Zhang J, McIntosh BE, Wang B, Brown ME, Probasco MD, Webster S, 
            Duffin B, Zhou Y, Guo LW, Burlingham WJ, Kent C, Ferris M, Thomson 
            JA
  TITLE     A Human Pluripotent Stem Cell-Based Screen for Smooth Muscle Cell 
            Differentiation and Maturation Identifies Inhibitors of Intimal 
            Hyperplasia.
  JOURNAL   Stem Cell Reports. 2019 Jun 11;12(6):1269-1281. doi: 
            10.1016/j.stemcr.2019.04.013. Epub 2019 May 9.
  PUBMED    31080110
REFERENCE   2  (bases 1 to 9882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2019.04.013"; journalName: "Stem Cell Reports"; 
            date: "2019-06-11- 11"; volume: "12"; issue: "6"; pages: "1269-1281"
COMMENT     Sequence Label: MYH11-NanoLuc-2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..9882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             566..1078
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1088..1144
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      1160..1169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1166..2596
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    2718..2839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2755..2774)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2809..2828
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(2851..2884)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        2903..3402
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2938..2961)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3335..3354
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        3419..3466
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3485..4288
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3548..3567)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4158..4177
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(4427..4460)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      complement(5255..5843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5335..5354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6014..6805,6806..6874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6637..6656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6875..6979)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(join(7047..8381,8382..8468))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYKVPEFDSSTIKNISSAKGLDVWDSWPLQNTDGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(8469..8914)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     protein_bind    8929..8962
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             8991..9650
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9057..9076)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      9671..9765
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9801..9836)
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
ORIGIN
        1 gcatgcctgc aggtcgaccc tgggtttgag caattctgcc tgagcctccc aagtggctgg
       61 gattacaggt gcacaccacc acacctggct aatttttgta ttttcagtag agacggggtt
      121 tcaccatgtt ggccaggctg gtcttgaact cctggcctca agtgatttgc ccacctcggc
      181 ctcccacagt gttgggttta caggtgtgag ccaccttgtc cagccctact tcctgcctcc
      241 tgagcactgg gtagtatttc acagtgtgga aatagcatgg cttatgtatc cagctgtatg
      301 ttggtggaca tttgggttgt ctgacttttg gtattttagg cgatgacaca catccctttc
      361 tgaagggcct ttgggtggtc tctgttcttt gaccacatac acaatactgc aggtctttgt
      421 acttggtggg atgtctctgc agaccgagtt cctagacagg gcaaggtgag tggttggtct
      481 gtcttccggg cttggttgtg gtctgattct gaccctgtta ccttattcca caggggacca
      541 acaaggcacc atggcgttgg atccaatggt cttcacactc gaagatttcg ttggggactg
      601 gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt
      661 gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa
      721 tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat
      781 gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt
      841 gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt
      901 cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac
      961 cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct
     1021 gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcggg
     1081 aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg
     1141 acctacccgg gtaccggtcg ccaccatggt gagcaagggc gaggaggtca tcaaagagtt
     1201 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
     1261 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1321 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
     1381 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
     1441 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
     1501 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
     1561 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     1621 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     1681 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     1741 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     1801 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag
     1861 caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa
     1921 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     1981 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     2041 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     2101 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     2161 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     2221 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     2281 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     2341 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     2401 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     2461 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     2521 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga
     2581 cgagctgtac aagtaggcgg ccgcgactct agatcataat cagccatacc acatttgtag
     2641 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     2701 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2761 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2821 aactcatcaa tgtatcttaa ggcgagatct gaagttccta tactttctag agaataggaa
     2881 cttcacgaag ttatattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     2941 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     3001 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cgtcgcgcca
     3061 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     3121 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     3181 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     3241 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     3301 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     3361 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cagcacgtgt
     3421 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     3481 aaccatggga tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3541 ggagaggcta ttcggctatg actgggcaca acagacgatc ggctgctctg atgccgccgt
     3601 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3661 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3721 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3781 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     3841 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3901 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     3961 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4021 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4081 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4141 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4201 ggctgaccgc ttcctcgtgc tttacggtat cgccgccccc gattcgcagc gcatcgcctt
     4261 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaata aagaccgacc
     4321 aagcgacgtc tgagagctcc ctggcgaatt cggtaccaat aaaagagctt tattttcatg
     4381 atctgtgtgt tggtttttgt gtgcggcgcg ataacttcgt atagctgaag ttcctatact
     4441 ttctagagaa taggaacttc gctagcatca acagcccagt ggcccaggct gactgggccg
     4501 ccaagagact cgtctgggtc ccctcggaga agcagggctt cgaggcagcc agcattaagg
     4561 aggagaaggg ggatgaggtg gttgtggagc tggtggagaa tggcaagaag gtcacggttg
     4621 ggaaagatga catccagaag atgaacccac ccaagttctc caaggtggag gacatggcgg
     4681 agctgacgtg cctcaacgaa gcctccgtgc tacacaacct gagggagcgg tacttctcag
     4741 ggctaatata tgtgagtatt gcaggcagcc aggtacctac tcctggcctc ataaggaaag
     4801 taggcattag aaatgtgttg ggagggctgg gcacagtggc tcatgcctgt actcccagca
     4861 ctttgggagg ccgaggtggg cagatcacct gaggtcagaa gttcgagacc acgctggcca
     4921 acatgatgaa atgccgtctc tactaaaaat acaaaaagta gccaagggtg gtggtgcgca
     4981 tctgtaatcc cagctactgg ggaggctggg gcagaggaat tgcttgaatc caggaggtgg
     5041 aggtgcagtg agccaagact gtaccactgc actccagcct gggtgacaga ataagactct
     5101 gtctcaaaaa aaaaaaaaaa aaaggaaatg ggaggctgac gcgtaaggcg gtaatacggt
     5161 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5221 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5281 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5341 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5401 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5461 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5521 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5581 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5641 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5701 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5761 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5821 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5881 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5941 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6001 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6061 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6121 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6181 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6241 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6301 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6361 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6421 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6481 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6541 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6601 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6661 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6721 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6781 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6841 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6901 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6961 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc aatgccaata
     7021 ggatatcggc attttctttt gcgtttttat ttgttaactg ttaattgtcc ttgttcaagg
     7081 atgctgtctt tgacaacaga tgttttcttg cctttgatgt tcagcaggaa gctaggcgca
     7141 aacgttgatt gtttgtctgc gtagaatcct ctgtttgtca tatagcttgt aatcacgaca
     7201 ttgtttcctt tcgcttgagg tacagcgaag tgtgagtaag taaaggttac atcgttagga
     7261 tcaagatcca tttttaacac aaggccagtt ttgttcagcg gcttgtatgg gccagttaaa
     7321 gaattagaaa cataaccaag catgtaaata tcgttagacg taatgccgtc aatcgtcatt
     7381 tttgatccgc gggagtcagt gaacaggtac catttgccgt tcattttaaa gacgttcgcg
     7441 cgttcaattt catctgttac tgtgttagat gcaatcagcg gtttcatcac ttttttcagt
     7501 gtgtaatcat cgtttagctc aatcataccg agagcgccgt ttgctaactc agccgtgcgt
     7561 tttttatcgc tttgcagaag tttttgactt tcttgacgga agaatgatgt gcttttgcca
     7621 tagtatgctt tgttaaataa agattcttcg ccttggtagc catcttcagt tccagtgttt
     7681 gcttcaaata ctaagtattt gtggccttta tcttctacgt agtgaggatc tctcagcgta
     7741 tggttgtcgc ctgagctgta gttgccttca tcgatgaact gctgtacatt ttgatacgtt
     7801 tttccgtcac cgtcaaagat tgatttataa tcctctacac cgttgatgtt caaagagctg
     7861 tctgatgctg atacgttaac ttgtgcagtt gtcagtgttt gtttgccgta atgtttaccg
     7921 gagaaatcag tgtagaataa acggattttt ccgtcagatg taaatgtggc tgaacctgac
     7981 cattcttgtg tttggtcttt taggatagaa tcatttgcat cgaatttgtc gctgtcttta
     8041 aagacgcggc cagcgttttt ccagctgtca atagaagttt cgccgacttt ttgatagaac
     8101 atgtaaatcg atgtgtcatc cgcattttta ggatctccgg ctaatgcaaa gacgatgtgg
     8161 tagccgtgat agtttgcgac agtgccgtca gtgttttgta atggccagct gtcccaaacg
     8221 tccaggcctt ttgcagaaga gatattttta attgtggacg aatcgaactc aggaacttta
     8281 tatttttcat ttttttgctg ttcagggatt tgcagcatat catggcgtgt aatatgggaa
     8341 atgccgtatg tttccttata tggcttttgg ttcgtttctt tcgcaaacgc ttgagttgcg
     8401 cctcctgcca gcagtgcggt agtaaaggtt aatactgttg cttgttttgc aaactttttg
     8461 atgttcatcg ttcatgtctc cttttttatg tactgtgtta gcggtctgct tcttccagcc
     8521 ctcctgtttg aagatggcaa gttagttacg cacaataaaa aaagacctaa aatatgtaag
     8581 gggtgacgcc aaagtataca ctttgccctt tacacatttt aggtcttgcc tgctttatca
     8641 gtaacaaacc cgcgcgattt acttttcgac ctcattctat tagactctcg tttggattgc
     8701 aactggtcta ttttcctctt ttgtttgata gaaaatcata aaaggatttg cagactacgg
     8761 gcctaaagaa ctaaaaaatc tatctgtttc ttttcattct ctgtattttt tatagtttct
     8821 gttgcatggg cataaagttg cctttttaat cacaattcag aaaatatcat aatatctcat
     8881 ttcactaaat aatagtgaac ggcaggtata tgtggacgtc gcggccgcat aacttcgtat
     8941 aatgtatgct atacgaagtt atggatcttt caggagctaa ggaagctaaa atggagaaaa
     9001 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     9061 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     9121 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     9181 cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga
     9241 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     9301 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     9361 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     9421 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     9481 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     9541 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     9601 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgatatcg
     9661 agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
     9721 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag cactagtcat
     9781 gaccaggatc tcctaggcta gttgtgatta tgattatgat tatgattatg attatgacca
     9841 cttacctggg cccgcgcgcc aaacgaatgg tctagaaagc tt
//
