LOCUS       sequence-268970-       10473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human Follistatin protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Guha TK, Pichavant C, Calos MP
  TITLE     Plasmid-Mediated Gene Therapy in Mouse Models of Limb Girdle 
            Muscular Dystrophy.
  JOURNAL   Mol Ther Methods Clin Dev. 2019 Oct 14;15:294-304. doi: 
            10.1016/j.omtm.2019.10.002. eCollection 2019 Dec 13.
  PUBMED    31890729
REFERENCE   2  (bases 1 to 10473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2019-10-14"; volume: "15:294-304. doi"; 
            pages: " 10.1016/j.omtm.2019.10.002. eCollection 2019 Dec 13"
COMMENT     Sequence Label: p2attB-FST344-HA-CPL
FEATURES             Location/Qualifiers
     source          1..10473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          525..1539
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1463..1485
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1547..1566
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2632..2658
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2665..2721
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2722..3429
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2867..2885)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3135..3154
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3436..3489
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3496..4092
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3496..3515)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3992..4012
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4108..4164
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4165..5817
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4231..4249)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(5894..5913)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5959..6014
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6013..6032)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6357..6376)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6401..6448
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    6619..6668
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     primer_bind     complement(6681..6698)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6687..6911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             8318..9112
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8318..9112
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8372..8391)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8982..9001
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      9437..10025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9926..9945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    10089..10122
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    10150..10219
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
       61 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      301 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      361 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      421 ggggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      481 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
      541 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
      601 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
      661 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
      721 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
      781 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
      841 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
      901 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
      961 cagggggtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
     1021 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
     1081 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
     1141 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
     1201 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
     1261 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     1321 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     1381 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     1441 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1501 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1561 attgtgctgt ctcatcattt tggcaaagaa ttcggtacca tggtccgcgc gaggcaccag
     1621 ccgggtgggc tttgcctcct gctgctgctg ctctgccagt tcatggagga ccgcagtgcc
     1681 caggctggga actgctggct ccgtcaagcg aagaacggcc gctgccaggt cctgtacaag
     1741 accgaactga gcaaggagga gtgctgcagc accggccggc tgagcacctc gtggaccgag
     1801 gaggacgtga atgacaacac actcttcaag tggatgattt tcaacggggg cgcccccaac
     1861 tgcatcccct gtaaagaaac gtgtgagaac gtggactgtg gacctgggaa aaaatgccga
     1921 atgaacaaga agaacaaacc ccgctgcgtc tgcgccccgg attgttccaa catcacctgg
     1981 aagggtccag tctgcgggct ggatgggaaa acctaccgca atgaatgtgc actcctaaag
     2041 gcaagatgta aagagcagcc agaactggaa gtccagtacc aaggcagatg taaaaagact
     2101 tgtcgggatg ttttctgtcc aggcagctcc acatgtgtgg tggaccagac caataatgcc
     2161 tactgtgtga cctgtaatcg gatttgccca gagcctgctt cctctgagca atatctctgt
     2221 gggaatgatg gagtcaccta ctccagtgcc tgccacctga gaaaggctac ctgcctgctg
     2281 ggcagatcta ttggattagc ctatgaggga aagtgtatca aagcaaagtc ctgtgaagat
     2341 atccagtgca ctggtgggaa aaaatgttta tgggatttca aggttgggag aggccggtgt
     2401 tccctctgtg atgagctgtg ccctgacagt aagtcggatg agcctgtctg tgccagtgac
     2461 aatgccactt atgccagcga gtgtgccatg aaggaagctg cctgctcctc aggtgtgcta
     2521 ctggaagtaa agcactccgg atcttgcaac tccatttcgg aagacaccga ggaagaggag
     2581 gaagatgaag accaggacta cagctttcct atatcttcta ttctagagtg gtacccatac
     2641 gacgtcccag actacgctac gcgtgccacg aacttctctc tgttaaagca agcaggagac
     2701 gtggaagaaa accccggtcc tatggtgagc aagggcgagg aggataacat ggccatcatc
     2761 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     2821 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     2881 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     2941 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     3001 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     3061 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     3121 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     3181 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     3241 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     3301 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     3361 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     3421 ctgtacaaga ccggtgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     3481 cccggccctt ccggtatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     3541 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     3601 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     3661 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     3721 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     3781 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     3841 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     3901 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     3961 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4021 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4081 aagcccggtg ccaccggtgg atccggtgcc acgaacttct ctctgttaaa gcaagcagga
     4141 gacgtggaag aaaaccccgg tcctatggaa gacgccaaaa acataaagaa aggcccggcg
     4201 ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
     4261 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact
     4321 tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
     4381 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
     4441 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
     4501 gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg
     4561 ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg
     4621 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
     4681 cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt
     4741 gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat
     4801 agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt
     4861 ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca
     4921 ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg
     4981 tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc
     5041 tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt
     5101 gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat
     5161 ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca
     5221 cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag
     5281 gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg
     5341 agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt
     5401 gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc
     5461 ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct
     5521 gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt
     5581 cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg
     5641 atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg
     5701 cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca
     5761 agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaagcg
     5821 gccgcgtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     5881 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     5941 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     6001 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     6061 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     6121 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     6181 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     6241 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     6301 tactcccagt catagctgtc cctcttctct ctagaggatc ctagggggag gctaactgaa
     6361 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     6421 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     6481 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     6541 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     6601 ggccctgcca tagcctcgtc ggccggcttg tcgacgacgg cggtctccgt cgtcaggatc
     6661 atccgggcca cacgcctagc cctcgactgt gccttctagt tgccagccat ctgttgtttg
     6721 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     6781 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6841 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     6901 gggctctatg ggaattcaaa ctagagggac agcccccccc caaagccccc agggatgtaa
     6961 ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg
     7021 cgctcccccc gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg
     7081 gcacaggatc gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg
     7141 gggatacggg gaaaaagctt taggctgaaa gagagattta gaatgacaga atcatagaac
     7201 ggcctgggtt gcaaaggagc acagtgctca tccagatcca accccctgct atgtgcaggg
     7261 tcatcaacca gcagcccagg ctgcccagag ccacatccag cctggccttg aatgcctgca
     7321 gggatggggc atccacagcc tccttgggca acctgttcag tgcgtcacca ccctctgggg
     7381 gaaaaactgc ctcctcatat ccaacccaaa cctcccctgt ctcagtgtaa agccattccc
     7441 ccttgtccta tcaaggggga gtttgctgtg acattgttgg tctggggtga cacatgtttg
     7501 ccaattcagt gcatcacgga gaggcagatc ttggggataa ggaagtgcag gacagcatgg
     7561 acgtgggaca tgcaggtgtt gagggctctg ggacactctc caagtcacag cgttcagaac
     7621 agccttaaag ataagaagat aggatagaag gacaaagagc aagttaaaac ccagcatgga
     7681 gaggagcaca aaaaggccac agacactgct ggtccctgtg tctgagcctg catgtttgat
     7741 ggtgtctgga tgcaagcaga aggggtggaa gagcttgcct ggagagatac agctgggtca
     7801 gtaggactgg gacaggcagc tggagaattg ccatgtagat gttcatacaa tcgtcaaatc
     7861 atgaaggctg gaaaagccct ccaagatccc caagaccaac cccaacccac ccaccgtgcc
     7921 cactggccat gtccctcagt gccacatccc cacagttctt catcacctcc agggacggtg
     7981 acccccccac ctccgtgggc agctgtgcca ctgcagcacc gctctttgga gaaggtaaat
     8041 cttgctaaat ccagcccgac cctcccctgg cacaacgtaa ggccattatc tctcatccaa
     8101 ctccaggacg gagtcagtga ggatggggct ctagtttgcg cgcctcttct actgggcggt
     8161 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
     8221 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
     8281 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     8341 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     8401 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     8461 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     8521 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     8581 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     8641 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     8701 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     8761 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     8821 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     8881 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     8941 ctgtggccgg ctgggggtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     9001 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     9061 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattattaa
     9121 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     9181 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9241 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatagc
     9301 acgtgctaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9361 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10081 acatgttcat aacttcgtat aatgtatgct atacgaagtt atcttaaggt cgacgatgta
    10141 ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
    10201 gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat
    10261 cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt
    10321 cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag
    10381 cgggttctcg acggtcacgg cgggcatgtc gacctcgaga ttaatagtaa tcaattacgg
    10441 ggtcattagt tcatagccca tatatggagt tcc
//
