LOCUS       sequence-268975-       15455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15455)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX031
FEATURES             Location/Qualifiers
     source          1..15455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        124..469
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    1096..1272
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        1775..2120
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    2142..2166
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3140..3151
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3166..3190)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3219..3308
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      3342..3594
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(4023..4275)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4743..5768)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(4743..5768)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5778..6122)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6232..6249)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6232..6248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6241..6263)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6388..6407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6598..6619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    6866..6890
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(8486..9634)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9933..10727)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLLLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  10919..11686
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      12309..13020
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(13147..13518)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            13551..13660
                     /note="incP origin of transfer"
     primer_bind     13883..13902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14136..14153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(14341..14991)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aacgttgcga ttaagaattc
       61 gagctcggta cccccctact ccaaaaatgt caaagataca gtctcagaag accaaagggc
      121 tattgagact tttcaacaaa gggtaatttc gggaaacctc ctcggattcc attgcccagc
      181 tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca
      241 ttgcgataaa ggaaaggcta tcattcaaga tgcctctgcc gacagtggtc ccaaagatgg
      301 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      361 agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc
      421 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacag cccaagcttc
      481 gactctagag gatcccctta aatcgatatg gaacgagcta tacaaggaaa cgacgctagg
      541 gaacaagcta acagtgaacg ttgggatgga ggatcaggag gtaccacttc tcccttcaaa
      601 cttcctgacg aaagtccgag ttggactgag tggcggctac ataacgatga gacgaattcg
      661 aatcaagata atccccttgg tttcaaggaa agctggggtt tcgggaaagt tgtatttaag
      721 agatatctca gatacgacag gacggaagct tcactgcaca gagtccttgg atcttggacg
      781 ggagattcgg ttaactatgc agcatctcga tttttcggtt tcgaccagat cggatgtacc
      841 tatagtattc ggtttcgagg agttagtatc accgtttctg gagggtctcg aactcttcag
      901 catctctgtg agatggcaat tcggtctaag caagaactgc tacagcttgc cccaatcgaa
      961 gtggaaagta atgtatcaag aggatgccct gaaggtactc aaaccttcga aaaagaaagc
     1021 gagtaagtcg agggcgagct cgaattcggt acgctgaaat caccagtctc tctctacaaa
     1081 tctatctctc tctattttct ccataaataa tgtgtgagta gtttcccgat aagggaaatt
     1141 agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     1201 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact
     1261 aaaatccaga tctccctgca ggtccccaga ttagcctttt caatttcaga aagaatgcta
     1321 acccacagat ggttagagag gcttacgcag caggtctcat caagacgatc tacccgagca
     1381 ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca
     1441 ggactaactg catcaagaac acagagaaag atatatttct caagatcaga agtactattc
     1501 cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag attggagtct
     1561 ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa atagaggacc
     1621 taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg
     1681 acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata
     1741 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
     1801 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
     1861 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
     1921 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     1981 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     2041 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     2101 catttcattt ggagagaaca cgggggactc tagagttatc aacaagtttg tacaaaaaag
     2161 caggctccac cactagtatg acgtccggca ctcggctacc gacatggaag gagagggaga
     2221 acaacaagcg gagagagcgg cggcggaggg cgatcgccgc caagatcttc accggattaa
     2281 ggatgtacgg taactataag ctccctaagc actgtgataa taacgaggta ttgaaagctc
     2341 tttgtaaaga agctggttgg atcgttgaag atgatggaac cacttaccga aagggttgca
     2401 agccagtaca ccacatggat attattggtg gttcggcatc agtaagccct tgttcgtctt
     2461 accaactgag ccctggtgct tcatataacc caagtcccgc ttcatcatct attcccagcc
     2521 cggtctcatc ccattatatt gccaatgtcc aaaataattc tgatcccaac tcgctcattc
     2581 cttggcttaa gaacctatca tctggctcgt cgccttcctc atctaacttt ccccaccatc
     2641 tgtgcattcc tggagattct ataagtgctc ctgtgacacc accttcgagc tctcccactg
     2701 cccgtactcc taggatgaat gataattggg aaaatcccac ggccaattca acatggatac
     2761 aacatcatta tcccttcttg cccttctcaa cccctcctag tcctggtggt cgccaaaccc
     2821 caccagattc aggatggatt tctggcgttc aaactccaca ggatgggcct tcatctccca
     2881 ctttcagcct tgtctcatca aatccatttg gatttaagga gccactatca aatggagggt
     2941 ctcgaatgtg gacacctggg caaagtggga catgttcacc tgcagttgga gctggtatgg
     3001 accaaacagc tgatatacca atgtcggatg ttatctcagc agagttcgca tttggcagca
     3061 acgtgaaggg agtagtaaag ccatgggaag gagaaagaat tcacgaagag tgtgtcagtg
     3121 atgatcttga gcttacactt gggaactcca gcactagacc cggggaccca gctttcttgt
     3181 acaaagtggt tgataacagc gggttaatta acatctttta cccatacgat gttcctgact
     3241 atgcgggcta tccctatgac gtcccggact atgcaggatc ctatccatat gacgttccag
     3301 attacgctgc tcagtgcagc gcttagagct cgaatttccc cgatcgttca aacatttggc
     3361 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     3421 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     3481 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     3541 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     3601 tagcttcatc aacgcaagac atgcgcacga ccgtctgaca ggagaggaat ttccgacgag
     3661 cacagaaagg acttgctctt ggacgtaggc ctatttctca ggcacatgta tcaagtgttc
     3721 ggacgtgggt tttcgatggt gtatcagccg ccgccaactg ggagatgagg aggctttctt
     3781 ggggggcagt cagcagttca tttcacaaga cagaggaact tgtaaggaga tgcactgatt
     3841 tatcttggcg caaaccagca ggacgaatta gtgggaatag cccgcgaata tctaagttat
     3901 gcctgtcggc atgagcagaa acttccaatt cgaaacagtt tggagaggtt gtttttgggc
     3961 ataccttttg ttagtcagcc tctcgattgc tcatcgtcat tacacagtac cgaagtttga
     4021 tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     4081 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     4141 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     4201 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     4261 aatgtttgaa cgatctgctt cgacgcactc cttctttact ccaccatctc gtccttattg
     4321 aaaacgtggg tagcaccaaa acgaatcaag tcgctggaac tgaagttacc aatcacgctg
     4381 gatgatttgc cagttggatt aatcttgcct ttccccgcat gaataatatt gatgaatgca
     4441 tgcgtgaggg gtatttcgat tttggcaata gctgcaattg ccgcgacatc ctccaacgag
     4501 cataattctt cagaaaaata gcgatgttcc atgttgtcag ggcatgcatg atgcacgtta
     4561 tgaggtgacg gtgctaggca gtattccctc aaagtttcat agtcagtatc atattcatca
     4621 ttgcattcct gcaagagaga attgagacgc aatccacacg ctgcggcaac cttccggcgt
     4681 tcgtggtcta tttgctcttg gacgttgcaa acgtaagtgt tggatcccgg tcggcatcta
     4741 ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     4801 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     4861 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     4921 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     4981 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
     5041 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
     5101 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
     5161 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
     5221 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     5281 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
     5341 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     5401 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
     5461 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
     5521 aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     5581 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     5641 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     5701 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     5761 tttttcatat cggggtcgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
     5821 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     5881 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     5941 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     6001 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     6061 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     6121 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     6181 cgtgctccac catgttgacg gatctctagg acgcgtccta gaagctaatt cactggccgt
     6241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6361 acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6421 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6481 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     6541 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6601 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     6661 aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac
     6721 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     6781 gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt
     6841 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     6901 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     6961 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     7021 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     7081 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc
     7141 ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg
     7201 tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc
     7261 tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct
     7321 caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc
     7381 tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
     7441 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
     7501 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
     7561 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
     7621 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
     7681 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
     7741 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
     7801 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
     7861 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
     7921 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
     7981 acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc
     8041 tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc
     8101 tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg
     8161 gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg
     8221 gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc
     8281 acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct
     8341 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
     8401 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
     8461 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
     8521 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
     8581 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
     8641 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
     8701 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
     8761 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
     8821 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
     8881 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
     8941 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
     9001 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
     9061 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
     9121 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
     9181 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
     9241 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
     9301 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
     9361 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
     9421 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
     9481 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
     9541 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
     9601 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
     9661 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
     9721 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
     9781 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
     9841 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
     9901 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
     9961 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    10021 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    10081 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    10141 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    10201 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    10261 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    10321 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    10381 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    10441 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    10501 acctttggaa caggcagctt tccttccagc aatagcatca tgtccttttc ccgttccaca
    10561 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    10621 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    10681 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    10741 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    10801 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    10861 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
    10921 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
    10981 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
    11041 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
    11101 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
    11161 gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc
    11221 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
    11281 cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg
    11341 gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg
    11401 cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg
    11461 gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag
    11521 caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg
    11581 gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg
    11641 gcattatctg aacataaaac actatcaata agttggagtc attacccaat tatgatagaa
    11701 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
    11761 ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc
    11821 cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat
    11881 attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat
    11941 atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa
    12001 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc
    12061 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
    12121 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact
    12181 gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg
    12241 cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac
    12301 tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg
    12361 ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg
    12421 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
    12481 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
    12541 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
    12601 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
    12661 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
    12721 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
    12781 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
    12841 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
    12901 gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
    12961 catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc
    13021 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
    13081 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
    13141 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
    13201 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
    13261 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
    13321 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
    13381 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
    13441 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
    13501 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
    13561 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
    13621 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
    13681 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
    13741 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
    13801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    13861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    13921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    13981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    14041 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    14101 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    14161 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
    14221 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
    14281 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
    14341 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
    14401 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
    14461 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
    14521 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
    14581 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
    14641 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
    14701 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
    14761 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
    14821 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
    14881 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
    14941 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
    15001 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
    15061 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
    15121 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
    15181 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
    15241 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
    15301 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
    15361 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
    15421 atccgattat tctaataaac gctcttttct cttag
//
