LOCUS       sequence-268976-        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for knock-in of TdTomato into the TH locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Ahfeldt T, Ordureau A, Bell C, Sarrafha L, Sun C, Piccinotti S, 
            Grass T, Parfitt GM, Paulo JA, Yanagawa F, Uozumi T, Kiyota Y, 
            Harper JW, Rubin LL
  TITLE     Pathogenic Pathways in Early-Onset Autosomal Recessive Parkinson's 
            Disease Discovered Using Isogenic Human Dopaminergic Neurons.
  JOURNAL   Stem Cell Reports. 2019 Dec 20. pii: S2213-6711(19)30444-8. doi: 
            10.1016/j.stemcr.2019.12.005.
  PUBMED    31902706
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2019 Dec 20. pii: S2213-6711(19)30444-8. doi: 
            10.1016/j.stemcr.2019.12.005."
COMMENT     Sequence Label: pTAHR TH-p2a-TD:Tomato (floxed selection Puro)
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     80..98
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(136..158)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     258..277
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     471..493
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     485..502
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     486..502
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             956..1012
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1031..2461
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             complement(2957..2968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3089..3322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3348..3469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3562..3595)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3949..4160
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4173..4441
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4469..4478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5153..5206
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5207..5806
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5207..5226)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5703..5723
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5930..6051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6551..6584)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7091..7107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7091..7107)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7104..7126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7115..7131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7139..7145,7146..7163,7164..7169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7184..7205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7322..7339)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7493..8081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7573..7592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8252..9043,9044..9112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8875..8894
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9113..9205,1..12))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
       61 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      121 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      181 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      241 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
      301 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
      361 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      421 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      481 acgttgtaaa acgacggcca gtgaattcag ctagttcctg gaggaggccc agtggaggtt
      541 cagggaggga tggggtgccc ggcagtctct agtggaaaag gcgcctagcc tatctccccc
      601 atgaaccccc tcacccagcc ctggaagagg cctcagtgtc ccgcctgtga ccagttggct
      661 cagaaaagcc ctgggagctc tgagccactg tgaaggtgga aacgcggccc ctggcctccc
      721 ctctcctgga ggctgcagac tctgcccgcc agttgacgag ggctctgccg ctctcctccc
      781 caggagctat gcctcacgca tccagcgccc cttctccgtg aagttcgacc cgtacacgct
      841 ggccatcgac gtgctggaca gcccccaggc cgtgcggcgc tccctggagg gtgtccagga
      901 tgagctggac acccttgccc atgcgctgag tgccattggc gctagcggaa gcggagctac
      961 taacttcagc ctgttgaaac aagcagggga tgtcgaagag aatcccgggc cattgaagac
     1021 aaaactcgag atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt
     1081 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1141 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     1201 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
     1261 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1321 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
     1381 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
     1441 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     1501 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
     1561 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
     1621 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     1681 ctccgagggc cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag
     1741 ctccggcacc gcctcctccg aggacaacaa catggccgtc atcaaagagt tcatgcgctt
     1801 caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     1861 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     1921 gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa
     1981 gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga
     2041 gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca
     2101 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
     2161 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
     2221 cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct
     2281 ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta
     2341 cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2401 cgagcgctcc gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaagta
     2461 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     2521 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2581 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2641 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2701 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2761 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2821 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2881 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2941 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3001 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3061 ggccgcctcc ccggagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
     3121 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3181 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3241 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3301 tcagtgtgga aaatctctag cagtagtagg ttaattaaag tgagaggaac ttgtttattg
     3361 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3421 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     3481 tctagctatc ccgcccctaa ctaaaactag tattcgagct cggtacctcg cgaatgcatc
     3541 agatctttcc actcaaaaca tataacttcg tatagcatac attatacgaa gttatattca
     3601 aaaatctaga cagcggccgc cactcaaaat ttcttctata aagtaacaaa acttttatga
     3661 gggacagccc ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc
     3721 agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg
     3781 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg
     3841 cttctcgctg ctctttgagc ctgcagacac ctggggggat acggggaaaa ggcctccaag
     3901 gccttaattc ggccgcaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     3961 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     4021 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     4081 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     4141 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     4201 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     4261 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     4321 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     4381 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     4441 ccaagctgtg accggcgcct acgatatcgc caccatggcc agctccgagg atgtcatcaa
     4501 agagtttatg agatttaagg tcaagatgga gggaagcgtc aacggacacg agttcgagat
     4561 tgagggagaa ggagaaggcc ggccttacga gggcacacaa accgctaagc tcaaggtcac
     4621 aaaaggagga cccctcccct tctcctggga tattctgagc cctcagttcc agtacggaag
     4681 caaagcctat gttaaacacc ctgccgacat ccctgactat ctgaagctct ccttccctga
     4741 aggcttcaag tgggagagat tcatgaactt cgaggacgga ggcgtggtga cagtcacaca
     4801 agatagcacc ctccaggacg gagagtttat ttataaggtg aaactcagag gaaccaactt
     4861 cccctccgat ggccctgtca tgcaaaaaaa aacaatggga tgggaagcct ccaccgagag
     4921 aatgtatcct gaggatggcg ctctgaaagg cgaaattaaa atgagactga aactcaaaga
     4981 cggaggacac tacgatgccg aggtcaaaac aacctacaag gccaagaaac aagtgcagct
     5041 gcctggcgcc tacatgactg atattaaact cgacattatc agccataatg gggactacac
     5101 catcgtggaa caatatgaga gagctgaggg cagacatagc acaggcgctg gagagggcag
     5161 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa
     5221 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5281 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5341 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5401 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5461 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5521 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5581 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5641 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5701 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5761 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga
     5821 cgaaattccg cggttattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5881 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcagtta acttgtttat
     5941 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6001 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6061 gaattgactc aaatgatgtc aattagtcta tcagaagcta tctggtctcc cttccggggg
     6121 acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc
     6181 ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga
     6241 aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg
     6301 tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     6361 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     6421 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     6481 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga
     6541 tatcaaacat ataacttcgt atagcataca ttatacgaag ttattttatc acgtaagtag
     6601 aacatgaaat aacctagatc ggatcgtgca cggcgtccct gagggccctt cccaacctcc
     6661 cctggtcctg cactgtcccg gagctcaggc cctggtgagg ggctgggtcc cgggtgcccc
     6721 ccatgccctc cctgctgcca ggctcccact gcccctgcac ctgcttctca gcgcaacagc
     6781 tgtgtgtgcc cgtggtgagg ttgtgctgcc tgtggtgagg tcctgtcctg gctcccaggg
     6841 tcctggggct gctgcactgc cctccgccct tccctgacac tgtctgctgc cccaatcacc
     6901 gtcacaataa aagaaactgt ggtctctaca cctgcctggc cccacatctg tgccacagag
     6961 acagaccctg ggatcctcag actcccacac ccccacccca gcctcactca gaggtttcgc
     7021 cctggcctcc ttcctcctct gggagatggc tggatccccg tcgactgcat gcaagcttgg
     7081 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7141 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7201 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7261 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7321 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7381 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7441 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7501 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7561 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7621 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7681 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7741 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7801 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7861 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7921 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7981 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8041 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8101 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8161 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8221 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8281 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8341 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8401 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8461 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8521 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8581 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8641 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8701 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8761 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8821 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8881 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8941 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9001 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9061 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9181 aatgtattta gaaaaataaa caaat
//
