LOCUS       sequence-268977-        8126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal 
            myc-tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Ruppert C, Kroschewski R, Bahler M
  TITLE     Identification, characterization and cloning of myr 1, a mammalian 
            myosin-I.
  JOURNAL   J Cell Biol. 1993 Mar;120(6):1393-403. doi: 10.1083/jcb.120.6.1393.
  PUBMED    8449985
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb.120.6";
            journalName: "J Cell Biol"; date: "1993-03"; volume: "120"; issue: 
            "6"; pages: "1393-403"
COMMENT     Sequence Label: Myo 1b-myc
FEATURES             Location/Qualifiers
     source          1..8126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4414..4443
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4467..5089
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4666..4685)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5113..5133)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5118..5447
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5298..5433
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5485..5504)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5486..5504)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5518..5534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5518..5534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5531..5553)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5542..5558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5566..5572,5573..5590,5591..5596))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5611..5632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5749..5766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5920..6508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6000..6019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6679..7470,7471..7539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7302..7321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7540..7644)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7671..8099
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7758..7777)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7968..7989
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attcggcacg aggcacggtc ggctgagcgg cgccggatcc
      961 ctggcaaaac tgtggcacga accccgcatc gggtcggacc cgcgttcccc aggcgccggc
     1021 cagcgagcag cacgcggagg cggagcggca gagcggttgc cgggcgagct ggaaaccatg
     1081 gccaagaagg aggtaaaatc ctcacttctg gacaatatga tcggcgttgg agatacggtt
     1141 ctcttggagc ctctcaacga ggagacgttc atcgacaacc tgaagaagcg cttcgatcac
     1201 aatgagatat atacatacat cgggagtgtg gttatatctg ttaaccccta ccggtccttg
     1261 cccatttatt caccagagaa agtggaagat tacagaaata gaaacttcta tgaactgagc
     1321 cctcacatct ttgctttgtc ggatgaagca tacagatccc tccgtgatca agataaagat
     1381 cagtgcattc tcattactgg ggagagtgga gcgggaaaaa cagaggccag caagcttgtg
     1441 atgtcctatg tcgctgcagt gtgtggaaaa ggagcagaag ttaatcaggt caaggaacag
     1501 cttctccagt ccacccctgt cctggaagct tttggaaatg ccaagactgt aaggaatgac
     1561 aattcatcca gattcggcaa atacatggat attgaatttg actttaaagg cgatccgctg
     1621 ggaggagtga taagtaacta tcttttagag aaatctcgtg ttgttaagca gccgagaggt
     1681 gaaagaaact tccacgtgtt ctatcagctg ctctcgggtg cctctgagga actcctccac
     1741 aagcttaaac tggagcggga tttcagcaga tacaactatc tgagtctgga ctctgccaaa
     1801 gtgaatggtg tggacgacgc tgccaatttc agaactgtca ggaatgccat gcagatcgtg
     1861 ggcttttcgg atcctgaggc cgagtctgtc ctggaggttg tggcagctgt cttgaaactg
     1921 gggaacatcg agttcaagcc tgaatcacga atgaacggtt tggatgaaag caaaatcaaa
     1981 gacaaaaatg agttaaaaga aatttgtgag ttgaccagca tcgatcaagt ggttttagaa
     2041 agagcgttca gtttccgaac ggttgaggcg aagcaggaga aagtttcaac cacattgaat
     2101 gtggctcagg cttattatgc ccgtgatgcc ctggctaaaa acctctacag ccggttgttt
     2161 tcatggttgg taaatcgaat caacgaaagc attaaggcac aaaccaaagt gagaaagaag
     2221 gtcatgggtg ttctggacat ttatggcttt gagatatttg aggacaacag cttcgagcag
     2281 ttcattatta attattgtaa tgaaaagctt caacaaatct tcattgaact gaccctcaaa
     2341 gaagagcaag aggaatatat tcgtgaggac atagaatgga ctcacattga ctacttcaac
     2401 aatgctatca tttgcgacct aatagaaaac aacacaaacg gaatcttggc catgctggat
     2461 gaagagtgcc tgagacccgg cactgttaca gatgagacct tcctggaaaa gctgaaccaa
     2521 gtctgtgcca cccaccagca cttcgagagc aggatgagca aatgttcccg cttcctcaat
     2581 gacacgaccc tgcctcacag ctgcttcaga atccagcatt atgctggcaa ggtgctatac
     2641 caggtggaag ggtttgttga caagaacaat gaccttctgt accgagacct gtctcaagcc
     2701 atgtggaagg ctggccatgc cctcatcaag tctctgttcc ctgaagggaa tcctgcaaag
     2761 gtcaacctga aaagacctcc tactgcaggg tcccagttca aggcgtcggt ggccaccctg
     2821 atgaaaaacc tgcagaccaa gaacccgaac tatatcaggt gcatcaagcc gaacgataaa
     2881 aaggccgctc acattttcag tgagagtctc gtgtgccatc agatccggta cctgggtctt
     2941 ctggagaacg ttcgagtgag acgggcaggc tatgccttca ggcaggccta tgaaccttgt
     3001 ctggaaagat acaagatgct ttgcaaacaa acatggcctc actggaaggg accagcaagg
     3061 tctggtgtgg aagttctgtt taatgagcta gagattcccg tggaagagta ctccttcggt
     3121 agatccaaga tattcatccg aaacccaagg acattattcc aattagaaga cctaaggaag
     3181 cagcggctgg aggacctggc cactctcatt cagaagattt atcgaggatg gaaatgtcgt
     3241 acacattttc tgctcatgaa aagaagccaa gtcgtgattg ccgcctggta caggagatac
     3301 gcgcaacaaa agcgatatca gcagataaag agttcagccc tggtgattca gtcttacatc
     3361 cggggctgga aggctagaaa aatcctgcgg gagctgaagc atcagaaacg ctgcaaggag
     3421 gcagccacca ccatcgcagc atactggcac ggcacccagg cacgaaggga attgaaacgc
     3481 ttgaaggagg aggctaggcg taagcatgca gttgctgtca tttgggctta ctggcttgga
     3541 ctgaaggtac gaagggagta caggaaattc tttagagcaa atgctggaaa gaaaatctat
     3601 gagtttacac ttcaaagaat tgtgcaaaaa tacttgttgg aaatgaaaaa taaaatgcct
     3661 tccttgtcac caatagataa aaattggcca tcaagacctt acctattctt ggattcgact
     3721 cacaaggagc taaaaaggat cttccacttg tggaggtgta aaaaatacag ggatcaattc
     3781 acagaccagc agaaacttat ttatgaagag aagctggagg ccagtgaact cttcaaagac
     3841 aagaaggctt tatatccttc tagtgttggt cagccattcc aaggagctta cctggaaatc
     3901 aacaagaacc caaaatataa gaaactcaaa gatgccattg aagagaagat cattattgct
     3961 gaggttgtga acaaaatcaa ccgggctaat gggaagagta catctcggat tttcctctta
     4021 acaaacaata accttctcct tgctgaccaa aagtcggggc agatcaagtc cgaggtcccc
     4081 ctggtggatg tgactaaggt ctccatgagt tcacagaatg atggtttctt tgcagtacac
     4141 ctcaaggagg gttcagaagc agctagtaag ggtgactttc tcttcagcag tgaccacctg
     4201 attgaaatgg caaccaagtt gtatcgcacg actcttagcc aaactaaaca gaagctcaat
     4261 atcgagattt ccgatgagtt cctggtacag ttcagacagg acaaagtatg tgtgaagttt
     4321 attcaaggca accagaaaaa cgggagcgtg ccaacctgca aacgaaagaa caacaggctt
     4381 cttgaagtcg ctgtcccacg gctgaattct cgggagcaga agctgatcag cgaggaagac
     4441 ctgctgtgat gaatctagag gatcccgggt ggcatccctg tgacccctcc ccagtgcctc
     4501 tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt
     4561 gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat
     4621 ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa
     4681 gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga
     4741 ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct
     4801 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact
     4861 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa
     4921 ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag gaggacgtcc
     4981 agacacagca taggctacct ggccatgccc aaccggtggg acatttgagt tgcttgcttg
     5041 gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattg ggtacgcggc
     5101 cagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5161 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5221 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5281 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5341 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5401 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg
     5461 aactgaaaaa ccagaaagtt aattccctat agtgagtcgt attaaattcg taatcatgtc
     5521 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5581 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5641 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5701 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5761 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5821 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6361 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6661 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6721 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6781 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6841 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6901 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6961 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7021 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7081 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7141 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7201 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7261 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7321 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7381 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7441 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7501 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7561 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7621 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
     7681 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7741 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7801 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7861 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7921 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7981 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8041 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8101 taacaaaata ttaacgctta caattt
//
