LOCUS       sequence-269007-       10238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CHO codon-optimized dCas9 fused to VPR for CRISPR 
            activation. 2A linked ZsGreen1 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Karottki KJC, Hefzi H, Xiong K, Shamie I, Hansen AH, Li S, Pedersen 
            LE, Li S, Lee JS, Lee GM, Kildegaard HF, Lewis NE
  TITLE     Awakening dormant glycosyltransferases in CHO cells with CRISPRa.
  JOURNAL   Biotechnol Bioeng. 2020 Feb;117(2):593-598. doi: 10.1002/bit.27199. 
            Epub 2019 Nov 12.
  PUBMED    31631317
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27199";
            journalName: "Biotechnol Bioeng"; date: "2020-02"; volume: "117"; 
            issue: "2"; pages: "593-598"
COMMENT     Sequence Label: VPR_dCas9
FEATURES             Location/Qualifiers
     source          1..10238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(287..1078,1079..1147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     910..929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1148..1239)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1281..1300
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1306..1325
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     enhancer        1538..1917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1918..2121
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2072..2092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2176..2185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2209..2358
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             2383..2403
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2833..3189
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             3208..3777
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             join(3787..3810,3811..3813,3814..6300,6301..6303,
                     6304..7887)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7900..7920
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7939..7992
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7993..8685
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     polyA_signal    8830..9054
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9529..9546)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9529..9545)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9644..9661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9815..10238,1..165))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9895..9914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
       61 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      121 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      181 ttttctacgg ggtctgacgc tcagtggaac gacgcgcgcg taactcacgt taagggattt
      241 tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgcttac caatgcttaa
      301 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      361 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagc gctgcgatga
      421 taccgcgaga accacgctca ccggctccgg atttatcagc aataaaccag ccagccggaa
      481 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
      541 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccatcg
      601 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      661 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      721 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
      781 cgctacataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
      841 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
      901 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
      961 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1021 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1081 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1141 tactcatatt cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1201 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggtcagt gttacaacca
     1261 attaaccaat tctgaacatt atcgcgagcc catttatacc tgaatatggc tcataacacc
     1321 ccttgtttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
     1381 gtgaaacgcc gtagcgccga tggtagtgtg gggactcccc atgcgagagt agggaactgc
     1441 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgcc cgggctaatt
     1501 atggggtgtc gcccttattc gactcagtcg gtgtgttgac attgattatt gactagttat
     1561 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     1621 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     1681 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     1741 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     1801 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     1861 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     1921 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     1981 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2041 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2101 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
     2161 gaaataagca gcgtcgccac catggaggcc agcggttccg gacgggctga cgcattggac
     2221 gattttgatc tggatatgct gggaagtgac gccctcgatg attttgacct tgacatgctt
     2281 ggttcggatg cccttgatga ctttgacctc gacatgctcg gcagtgacgc ccttgatgat
     2341 ttcgacctgg acatgctgat taactctaga agttccggat ctccgaaaaa gaaacgcaaa
     2401 gttggtagcc agtacctgcc cgacaccgac gaccggcacc ggatcgagga aaagcggaag
     2461 cggacctacg agacattcaa gagcatcatg aagaagtccc ccttcagcgg ccccaccgac
     2521 cctaggcctc cacctagaag aatcgccgtg cccagcagat ccagcgccag cgtgccaaaa
     2581 cctgcccccc agccttaccc cttcaccagc agcctgagca ccatcaacta cgacgagttc
     2641 cctaccatgg tgttccccag cggccagatc tctcaggcct ctgctctggc cccagcccct
     2701 cctcaggtgc tgcctcaggc tcctgctcct gcaccagctc cagccatggt gtctgcactg
     2761 gctcaggcac cagcacccgt gcctgtgctg gctcctggac ctccacaggc tgtggctcca
     2821 ccagccccta aacctacaca ggccggcgag ggcacactgt ctgaagctct gctgcagctg
     2881 cagttcgacg acgaggatct gggagccctg ctgggaaaca gcaccgatcc tgccgtgttc
     2941 accgacctgg ccagcgtgga caacagcgag ttccagcagc tgctgaacca gggcatccct
     3001 gtggcccctc acaccaccga gcccatgctg atggaatacc ccgaggccat cacccggctc
     3061 gtgacaggcg ctcagaggcc tcctgatcca gctcctgccc ctctgggagc accaggcctg
     3121 cctaatggac tgctgtctgg cgacgaggac ttcagctcta tcgccgatat ggatttctca
     3181 gccttgctgg gctctggcag cggcagccgg gattccaggg aagggatgtt tttgccgaag
     3241 cctgaggccg gctccgctat tagtgacgtg tttgagggcc gcgaggtgtg ccagccaaaa
     3301 cgaatccggc catttcatcc tccaggaagt ccatgggcca accgcccact ccccgccagc
     3361 ctcgcaccaa caccaaccgg tccagtacat gagccagtcg ggtcactgac cccggcacca
     3421 gtccctcagc cactggatcc agcgcccgca gtgactcccg aggccagtca cctgttggag
     3481 gatcccgatg aagagacgag ccaggctgtc aaagcccttc gggagatggc cgatactgtg
     3541 attccccaga aggaagaggc tgcaatctgt ggccaaatgg acctttccca tccgccccca
     3601 aggggccatc tggatgagct gacaaccaca cttgagtcca tgaccgagga tctgaacctg
     3661 gactcacccc tgaccccgga attgaacgag attctggata ccttcctgaa cgacgagtgc
     3721 ctcttgcatg ccatgcatat cagcacagga ctgtccatct tcgacacatc tctgtttaaa
     3781 gcctatgaca agaaatactc catcggcctt gccatcggga ccaacagcgt gggttgggcc
     3841 gtgatcactg acgaatacaa agtgccatcg aaaaagttta aggtgctggg caatactgac
     3901 agacatagca tcaagaaaaa cctgattgga gccctcctgt tcgactccgg ggaaaccgcc
     3961 gaagccactc gcctgaaacg gaccgcacgc cggaggtaca ctcgccggaa gaatagaatc
     4021 tgctaccttc aagagatctt tagcaacgag atggccaaag tggacgattc gttcttccac
     4081 cggctggagg agtcattcct ggtcgaagag gacaaaaagc atgagagaca tccgatcttc
     4141 ggtaacattg tggatgaggt cgcctaccac gagaagtacc ccactatcta ccatttgcgc
     4201 aagaagttgg tcgactcgac tgataaggcc gacctcagac tcatctacct cgctttggca
     4261 cacatgatca agtttagggg tcacttcctg attgaagggg atctgaaccc agacaactcg
     4321 gatgtggata aactgtttat ccagctggtc cagacttaca atcagctctt tgaggagaac
     4381 ccgatcaatg cctccggcgt ggatgctaag gcaatcctgt ccgcacgctt gtcaaagagc
     4441 cggcggctcg aaaatctgat cgcacagctt ccgggagaaa agaaaaacgg actgtttgga
     4501 aaccttatcg ccctctcgct gggactcact cctaacttca aatccaattt tgatttggcc
     4561 gaggatgcca agctgcagct gtcgaaggac acctacgacg atgacctcga caatcttctg
     4621 gcccagatcg gggaccagta cgccgatctg ttcctcgcgg ccaaaaacct gtcagacgca
     4681 atcttgctga gcgatatctt gagggtcaat accgaaatca ctaaggctcc attgtcagca
     4741 tcgatgatca agaggtacga tgaacaccat caggacctga ccctgctcaa ggcactggtg
     4801 cggcagcaac tgccggaaaa gtacaaggag atcttcttcg atcaaagcaa gaatgggtac
     4861 gcagggtaca tcgatggtgg agcatcacaa gaagagttct acaaattcat caagcccatc
     4921 cttgaaaaga tggacgggac ggaagaactg ctggtgaagc tgaatcggga agatctgctg
     4981 cggaagcagc gcaccttcga caatggttcc atcccacacc aaatccatct cggcgaactg
     5041 cacgcgatcc ttcgccggca ggaagatttc tacccgttct tgaaggataa cagagaaaag
     5101 atcgagaaaa tcctgacctt tagaatcccg tactacgtgg gcccgttggc tcgcggaaac
     5161 tcaagattcg cctggatgac tagaaaatcc gaagaaacga ttaccccgtg gaactttgaa
     5221 gaggtcgtcg acaagggagc ctcggcacag tcgttcatcg aacggatgac caatttcgac
     5281 aagaacctcc ccaacgaaaa ggtgctcccg aaacactcgc tgctgtatga gtacttcacg
     5341 gtctacaacg agctgaccaa ggtgaagtac gtgaccgaag gaatgcggaa gcccgctttt
     5401 ctgtccggtg aacagaaaaa ggccatcgtg gacctcctct tcaagactaa cagaaaggtg
     5461 accgtgaaac agcttaaaga ggactacttc aagaagatcg agtgttttga ctccgtggaa
     5521 atctcgggag tggaggatag gtttaacgct tcgctgggga cctaccatga ccttctcaag
     5581 atcatcaagg ataaggattt cctggacaac gaagaaaacg aggacatcct tgaggacatc
     5641 gtgttgacct tgaccctgtt tgaggatcgg gaaatgatcg aggagagact gaaaacttac
     5701 gcgcatcttt tcgacgacaa agtgatgaag cagctgaaaa gaagaagata cactggctgg
     5761 ggacggctgt cgaggaaact gattaacgga attagggata aacaaagcgg aaagactatc
     5821 ctcgattttc tcaagtcgga cggcttcgcc aatcggaact ttatgcagct catccacgac
     5881 gatagcctga ccttcaagga agatatccaa aaagcccaag tgtccggcca gggagatagc
     5941 ttgcacgagc acattgctaa cctggccggt tcaccagcca ttaagaaggg aatcctccaa
     6001 accgtgaagg tggtcgacga actcgtcaag gtgatgggcc gccataagcc agagaacatc
     6061 gtgatcgaga tggctcgcga aaatcagact actcagaagg gtcaaaagaa ctcccgcgag
     6121 cgcatgaagc gcatcgaaga aggaattaag gagctgggct cacaaatcct caaagaacat
     6181 cctgtcgaga acacccaact tcagaacgag aaactgtacc tctactatct ccaaaatggc
     6241 cgggacatgt acgtcgatca ggaattggat atcaaccgcc tctcagacta cgacgtggat
     6301 gctatcgtgc cgcagtcgtt tctcaaagat gatagcatcg acaacaaggt cctgacgagg
     6361 tcggataaga accgcgggaa atccgacaat gtgccttcgg aagaggtggt gaaaaagatg
     6421 aagaactact ggaggcaatt gcttaatgcc aaactgatca cccagcgcaa attcgacaac
     6481 cttactaagg ccgagcgcgg aggactgtcc gaactcgata aggcggggtt catcaaaaga
     6541 caattggtcg aaactcggca aattaccaag catgtcgctc agatcctcga ctcgcgcatg
     6601 aacactaagt atgatgagaa cgacaagctc attcgcgaag tgaaagtgat taccctcaaa
     6661 tcaaagctgg tgtcggactt cagaaaagac tttcaattct acaaggtgcg ggagattaac
     6721 aactaccacc acgcgcacga cgcctacctc aatgcagtgg tgggaaccgc cctcatcaag
     6781 aagtatccaa agctcgaaag cgagttcgtg tacggggact ataaagtgta cgatgtgcgg
     6841 aaaatgatcg caaagagcga gcaagagatc ggcaaagcaa cggccaaata cttcttctac
     6901 agcaacatta tgaacttttt caagaccgag attaccctgg cgaacggaga aatccggaag
     6961 cggcctttga tcgaaacgaa tggggaaact ggagaaatcg tgtgggacaa gggcagagac
     7021 tttgcgaccg tgagaaaggt gctgtccatg ccacaagtca acatcgtgaa gaaaactgaa
     7081 gtgcagactg gaggattttc caaggagtca atccttccga agcgcaacag cgacaagctg
     7141 atcgccagga agaaggattg ggaccccaag aagtacggtg gttttgattc acctactgtc
     7201 gcttacagcg tgctggtggt ggccaaggtg gaaaagggga agtcaaagaa attgaagtcg
     7261 gtgaaggaat tgctggggat tactatcatg gagaggagca gcttcgaaaa gaatcccatc
     7321 gacttcttgg aggccaaggg atacaaagag gtgaagaaag acctgatcat taagctgccg
     7381 aagtactcct tgttcgaact ggaaaacggc agaaagcgga tgctggcctc cgccggagag
     7441 ctgcagaagg gtaacgagct ggctctgccc agcaaatacg tgaatttcct ctacctggcc
     7501 tcccattacg agaagctcaa gggaagcccg gaggataatg agcaaaaaca actgttcgtc
     7561 gagcagcaca agcactacct cgacgagatc atcgaacaaa tctccgagtt ctcgaagcgg
     7621 gtgattctgg ccgacgcaaa ccttgataaa gtcctcagcg cctacaacaa gcaccgcgac
     7681 aaaccgatca gagaacaagc tgagaacatc atccacctgt tcacgctgac taacttggga
     7741 gcgcctgccg ccttcaagta cttcgacacc actatcgatc ggaaacggta cacttccact
     7801 aaggaagtgc tggacgcaac cctgatccat cagtccatca ccggacttta tgagactcgg
     7861 atcgatctca gccagcttgg cggtgattca agagctgacc cgaagaagaa gcgcaaggtc
     7921 agacgtcatg gcagtggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     7981 aatcctggcc caatggccca gtccaagcac ggcctgacca aggagatgac catgaagtac
     8041 cgcatggagg gctgcgtgga cggccacaag ttcgtgatca ccggcgaggg catcggctac
     8101 cccttcaagg gcaagcaggc catcaacctg tgcgtggtgg agggcggccc cttgcccttc
     8161 gccgaggaca tcttgtccgc cgccttcatg tacggcaacc gcgtgttcac cgagtacccc
     8221 caggacatcg tcgactactt caagaactcc tgccccgccg gctacacctg ggaccgctcc
     8281 ttcctgttcg aggacggcgc cgtgtgcatc tgcaacgccg acatcaccgt gagcgtggag
     8341 gagaactgca tgtaccacga gtccaagttc tacggcgtga acttccccgc cgacggcccc
     8401 gtgatgaaga agatgaccga caactgggag ccctcctgcg agaagatcat ccccgtgccc
     8461 aagcagggca tcttgaaggg cgacgtgagc atgtacctgc tgctgaagga cggtggccgc
     8521 ttgcgctgcc agttcgacac cgtgtacaag gccaagtccg tgccccgcaa gatgcccgac
     8581 tggcacttca tccagcacaa gctgacccgc gaggaccgca gcgacgccaa gaaccagaag
     8641 tggcacctga ccgagcacgc catcgcctcc ggctccgcct tgcccaagct tccgcggagc
     8701 catggcttcc cgccggcggt ggcggcgcag gatgatggca cgctgcccat gtcttgtgcc
     8761 caggagagcg ggatggaccg tcaccctgca gcctgtgctt ctgctaggat caatgtgtag
     8821 acacagtctc tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8881 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8941 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     9001 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggacttgc
     9061 gtagtgagtc gaataagggc gacacaaaat ttattctaaa tgcataataa atactgataa
     9121 catcttatag tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa
     9181 aactgatttt ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag
     9241 aaatattgta tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca
     9301 tcaatcgaaa aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt
     9361 taaataattc tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct
     9421 ctttccctaa actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg
     9481 attaacgatt actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt
     9541 tttacaacac agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc
     9601 ttagtttgat gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc
     9661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10201 taggcggtgc tacagagttc ttgaagtggt gggctaac
//
