LOCUS       sequence-269008-       12090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12090)
  AUTHORS   Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen 
            LL
  TITLE     Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.
  JOURNAL   Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 
            10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19.
  PUBMED    31757757
REFERENCE   2  (bases 1 to 12090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.10.024"; journalName: "Mol Cell"; date: 
            "2019-12-19- 19"; volume: "76"; issue: "6"; pages: "981-997.e7"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag
FEATURES             Location/Qualifiers
     source          1..12090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      6387..6396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6393..6395,6396..6398,6399..7109)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6393..6395,6396..6398,6399..7109)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6438..6459)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6699..6718)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7046..7067
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7137..7184
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7203..7268
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7275..7842
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7436..7453)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7663..7682
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7862..8461
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7862..7881)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8358..8378
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8480..9068
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8533..8553)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8951..8962)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9143..9376
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9495..9513)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9581..9685
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9686..9754,9755..10546)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9904..9923)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10717..11305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11206..11225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11459..11476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11716..11737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11752..11757,11758..11775,11776..11782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11790..11806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11795..11817
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11814..11830
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11814..11830
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11880..11899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcatc gagaagaaga
     3481 agagcttcgc caagggcatg ggcgtgaaga gcaccctggt gtccggctct aaggtgtaca
     3541 tgaccacatt tgccgagggc tccgacgcac ggctggagaa gatcgtggag ggcgatagca
     3601 tcagatccgt gaacgaggga gaggccttca gcgccgagat ggccgacaag aatgccggct
     3661 ataagatcgg caacgccaag ttttcccacc caaagggcta tgccgtggtg gccaacaatc
     3721 cactgtacac cggaccagtg cagcaggaca tgctgggcct gaaggagaca ctggagaaga
     3781 gatacttcgg cgagagcgcc gacggcaatg ataacatctg catccaggtc atccacaata
     3841 tcctggatat cgagaagatc ctggccgagt atatcaccaa cgccgcctac gccgtgaaca
     3901 atatctccgg cctggacaag gatatcatcg gcttcggcaa gttttctacc gtgtacacat
     3961 atgacgagtt caaggacccc gagcaccaca gggccgcctt taacaataac gacaagctga
     4021 tcaatgccat caaggcccag tacgacgagt tcgataattt tctggataac ccccggctgg
     4081 gctattttgg ccaggccttc ttttctaagg agggcagaaa ctatatcatc aattacggca
     4141 acgagtgtta cgacatcctg gccctgctga gcggactggc ccactgggtg gtggccaata
     4201 acgaggagga gtctaggatc agccgcacct ggctgtataa tctggacaag aatctggata
     4261 acgagtacat ctccacactg aactacctgt atgatcggat caccaatgag ctgacaaaca
     4321 gcttctccaa gaattctgcc gccaatgtga actacatcgc agagaccctg ggcatcaacc
     4381 cagcagagtt cgcagagcag tacttccggt tcagcatcat gaaggagcag aagaatctgg
     4441 gctttaacat cacaaagctg cgggaagtga tgctggacag aaaggatatg tccgagatcc
     4501 ggaagaacca caaggtgttc gattctatca gaaccaaggt gtatacaatg atggactttg
     4561 tgatctaccg gtactatatc gaggaggatg ccaaggtggc cgccgccaat aagagcctgc
     4621 ccgacaacga gaagtctctg agcgagaagg atatcttcgt gatcaatctg agaggctcct
     4681 ttaacgacga tcagaaggac gccctgtact atgatgaggc caatcggatc tggagaaagc
     4741 tggagaatat catgcacaac atcaaggagt tcaggggcaa caagacccgc gagtacaaga
     4801 agaaggacgc accaaggctg cctcgcatcc tgcctgcagg aagggacgtg agcgccttca
     4861 gcaagctgat gtatgccctg acaatgtttc tggacggcaa ggagatcaat gatctgctga
     4921 ccacactgat caataagttc gacaacatcc agtcctttct gaaagtgatg cctctgatcg
     4981 gcgtgaacgc caagttcgtg gaggagtacg ccttctttaa ggacagcgcc aagatcgccg
     5041 atgagctgag gctgatcaag tcctttgcca ggatgggaga gccaatcgca gacgcaagga
     5101 gagccatgta catcgatgcc atcagaatcc tgggcaccaa tctgtcttat gatgagctga
     5161 aggccctggc cgacaccttc agcctggacg agaatggcaa caagctgaag aagggcaagc
     5221 acggcatgcg caatttcatc atcaataacg tgatcagcaa caagaggttt cactatctga
     5281 tcagatacgg cgaccctgca cacctgcacg agatcgccaa gaacgaggcc gtggtgaagt
     5341 tcgtgctggg caggatcgcc gatatccaga agaagcaggg ccagaatggc aagaaccaga
     5401 tcgaccgcta ctatgagacc tgcatcggca aggataaggg caagtccgtg tctgagaagg
     5461 tggacgccct gaccaagatc atcacaggca tgaattatga ccagttcgat aagaagcgga
     5521 gcgtgatcga ggacaccgga agggagaacg cagagagaga gaagtttaag aagatcatca
     5581 gcctgtatct gacagtgatc taccacatcc tgaagaatat cgtgaatatc aacgccagat
     5641 acgtgatcgg cttccactgc gtggagcgcg atgcccagct gtataaggag aagggctacg
     5701 acatcaacct gaagaagctg gaggagaagg gctttagctc cgtgaccaag ctgtgcgcag
     5761 gaatcgacga gacagcaccc gacaagcgga aggatgtgga gaaggagatg gccgagagag
     5821 ccaaggagag catcgactcc ctggagtctg ccaatcctaa gctgtacgcc aactatatca
     5881 agtactccga tgagaagaag gccgaggagt tcacccggca gatcaataga gagaaggcca
     5941 agaccgccct gaacgcctac ctgcggaata caaagtggaa cgtgatcatc agggaggacc
     6001 tgctgcgcat cgataataag acctgtacac tgttcgccaa caaggccgtg gccctggagg
     6061 tggcccgcta tgtgcacgcc tacatcaatg acatcgccga ggtgaactcc tactttcagc
     6121 tgtaccacta tatcatgcag cggatcatca tgaacgagag atatgagaag tctagcggca
     6181 aggtgtctga gtacttcgac gccgtgaatg atgagaagaa gtataacgat agactgctga
     6241 agctgctgtg cgtgcctttc ggctactgta tcccaaggtt taagaatctg agcatcgagg
     6301 ccctgttcga caggaacgag gcagccaagt ttgataagga gaagaagaag gtgagcggca
     6361 actccagtct agaggcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
     6421 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     6481 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     6541 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     6601 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     6661 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     6721 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     6781 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     6841 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     6901 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     6961 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     7021 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     7081 tcactctcgg catggacgag ctgtacaagt cgaattctaa gccaggattc catatgaagc
     7141 gacctgccgc cacaaagaag gctggacagg ctaagaagaa gaaacaacgc gtgcagctaa
     7201 gcgactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
     7261 atgacaagta aggatccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     7321 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     7381 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     7441 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     7501 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     7561 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     7621 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     7681 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     7741 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     7801 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aaggatccac cggagcttac
     7861 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     7921 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     7981 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8041 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     8101 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8161 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     8221 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     8281 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     8341 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     8401 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     8461 acgcccatcg atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     8521 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     8581 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     8641 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8701 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8761 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     8821 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     8881 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8941 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     9001 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9061 ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     9121 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     9181 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9241 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9301 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9361 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     9421 aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc
     9481 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     9541 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     9601 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     9661 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     9721 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9781 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9841 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9901 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     9961 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    10021 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    10081 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    10141 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10201 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    10261 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    10321 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    10381 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    10441 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10501 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10561 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10621 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10681 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10741 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10801 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10861 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10921 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10981 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    11041 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    11101 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11161 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11221 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11281 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    11341 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    11401 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    11461 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    11521 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag
    11581 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    11641 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
    11701 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    11761 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    11821 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
    11881 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    11941 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    12001 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    12061 ttgttaaata tgtactacaa acttagtagt
//
