LOCUS       sequence-269026-       12464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen 
            LL
  TITLE     Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.
  JOURNAL   Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 
            10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19.
  PUBMED    31757757
REFERENCE   2  (bases 1 to 12464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.10.024"; journalName: "Mol Cell"; date: 
            "2019-12-19- 19"; volume: "76"; issue: "6"; pages: "981-997.e7"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag
FEATURES             Location/Qualifiers
     source          1..12464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3437..3457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      6761..6770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6767..6769,6770..6772,6773..7483)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6767..6769,6770..6772,6773..7483)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6812..6833)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7073..7092)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7420..7441
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7511..7558
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7577..7642
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7649..8216
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7810..7827)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8037..8056
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8236..8835
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8236..8255)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8732..8752
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8854..9442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8907..8927)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9325..9336)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9517..9750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9869..9887)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9955..10059
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10060..10128,10129..10920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10278..10297)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11091..11679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11580..11599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11833..11850
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12090..12111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12126..12131,12132..12149,12150..12156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12164..12180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12169..12191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12188..12204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12188..12204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12254..12273
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc accggtctgc
     3421 agctagcgcc accatgccca aaaagaagag gaaagtggag aagcccctgc tgcctaacgt
     3481 gtataccctg aagcacaagt tcttttgggg cgccttcctg aatatcgccc ggcacaacgc
     3541 ctttatcacc atctgccaca tcaatgagca gctgggcctg aaaaccccca gcaacgacga
     3601 taagatcgtg gacgtggtgt gcgagacatg gaacaatatc ctgaacaatg accacgatct
     3661 gctgaagaag tcccagctga ccgagctgat cctgaagcac ttcccttttc tgacagccat
     3721 gtgctaccac ccccctaaga aggagggcaa gaagaagggc caccagaagg agcagcagaa
     3781 ggagaaggag agcgaggcac agtcccaggc agaggccctg aacccatcca agctgatcga
     3841 ggccctggag atcctggtga atcagctgca ctctctgcgg aactactata gcgcctataa
     3901 gcacaagaag cccgacgccg agaaggatat cttcaagcac ctgtataagg ccttcgacgc
     3961 ctctctgaga atggtgaagg aggattataa ggcccacttc accgtgaatc tgacaaggga
     4021 ctttgcccac ctgaaccgca agggcaagaa caagcaggac aatcccgact tcaacagata
     4081 cagattcgag aaggatggct tctttaccga gtctggcctg ctgttcttta caaatctgtt
     4141 tctggacaag agggatgcct attggatgct gaagaaggtg tccggcttca aggcctctca
     4201 caagcagcgc gagaagatga ccacagaggt gttttgcagg agccgcatcc tgctgcccaa
     4261 gctgaggctg gagtcccgct acgaccacaa ccagatgctg ctggatatgc tgtctgagct
     4321 gagcagatgt cctaagctgc tgtatgagaa gctgagcgag gagaataaga agcacttcca
     4381 ggtggaggcc gacggctttc tggatgagat cgaggaggag cagaacccat tcaaggacac
     4441 cctgatccgg caccaggata gattccccta ctttgccctg aggtatctgg acctgaatga
     4501 gtccttcaag tctatccgct ttcaggtgga tctgggcaca taccactatt gtatctacga
     4561 caagaagatc ggcgatgagc aggagaagag gcacctgacc cgcacactgc tgtccttcgg
     4621 ccggctgcag gactttaccg agatcaacag accccaggag tggaaggccc tgaccaagga
     4681 cctggattac aaggagacat ccaatcagcc tttcatctct aagaccacac cacactatca
     4741 catcaccgac aacaagatcg gctttcggct gggcacaagc aaggagctgt acccctccct
     4801 ggagatcaag gatggcgcca atagaatcgc caagtaccct tataactccg gcttcgtggc
     4861 ccacgccttt atctctgtgc acgagctgct gcctctgatg ttctaccagc acctgaccgg
     4921 caagagcgag gacctgctga aggagacagt gcggcacatc cagagaatct ataaggactt
     4981 cgaggaggag cggatcaata ccatcgagga tctggagaag gcaaaccagg gcagactgcc
     5041 actgggagcc tttcccaagc agatgctggg cctgctgcag aataagcagc ctgatctgtc
     5101 cgagaaggcc aagatcaaga tcgagaagct gatcgccgag acaaagctgc tgtctcacag
     5161 gctgaacaca aagctgaaga gctccccaaa gctgggcaag cggagagaga agctgatcaa
     5221 gacaggcgtg ctggccgact ggctggtgaa ggatttcatg cgctttcagc ccgtggccta
     5281 cgacgcccag aatcagccta tcaagtctag caaggccaac tccaccgagt tctggtttat
     5341 caggcgcgcc ctggccctgt acggaggaga gaagaatagg ctggagggct atttcaagca
     5401 gaccaacctg atcggcaaca caaatccaca ccccttcctg aacaagttta attggaaggc
     5461 ctgcaggaat ctggtggatt tctaccagca gtatctggag cagcgcgaga agtttctgga
     5521 ggccatcaag aaccagccat gggagcccta ccagtattgc ctgctgctga agatccctaa
     5581 ggagaacagg aagaatctgg tgaagggatg ggagcaggga ggaatctctc tgccacgggg
     5641 cctgtttacc gaggccatca gagagacact gtctgaggac ctgatgctga gcaagccaat
     5701 ccgcaaggag atcaagaagc acggccgggt gggcttcatc agcagagcca tcaccctgta
     5761 ctttaaggag aagtatcagg ataagcacca gagcttctac aatctgtcct ataagctgga
     5821 ggcaaaggca ccactgctga agcgggagga gcactacgag tattggcagc agaacaagcc
     5881 tcagtctcca accgagagcc agaggctgga gctgcacaca agcgaccgct ggaaggatta
     5941 cctgctgtat aagagatggc agcacctgga gaagaagctg cggctgtacc ggaatcagga
     6001 cgtgatgctg tggctgatga ccctggagct gacaaagaac cacttcaagg agctgaacct
     6061 gaattatcac cagctgaagc tggagaatct ggccgtgaac gtgcaggagg ccgatgccaa
     6121 gctgaaccct ctgaatcaga ccctgcccat ggtgctgcct gtgaaggtgt accctgccac
     6181 agcctttggc gaggtgcagt accacaagac cccaatcagg acagtgtata tccgcgagga
     6241 gcacaccaag gccctgaaga tgggcaactt caaggccctg gtgaaggacc ggagactgaa
     6301 tggcctgttc tcttttatca aggaggagaa cgatacacag aagcacccaa tcagccagct
     6361 gcggctgagg cgcgagctgg agatctacca gtctctgaga gtggacgcct tcaaggagac
     6421 actgagcctg gaggagaagc tgctgaataa gcacacatcc ctgtcctctc tggagaacga
     6481 gtttagggcc ctgctggagg agtggaagaa ggagtatgcc gccagctcca tggtgacaga
     6541 cgagcacatc gccttcatcg ccagcgtgcg caatgccttc tgcgccaacc agtacccctt
     6601 ctacaaggag gccctgcacg cccctatccc actgttcacc gtggcccagc ccaccacaga
     6661 ggagaaggat ggcctgggaa tcgcagaggc cctgctgaag gtgctgaggg agtactgtga
     6721 gatcgtgaag tcccagatcg gtctagaggc gctaccggtc gccaccatgg tgagcaaggg
     6781 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     6841 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     6901 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     6961 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     7021 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     7081 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     7141 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     7201 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     7261 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     7321 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     7381 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     7441 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtcgaatt ctaagccagg
     7501 attccatatg aagcgacctg ccgccacaaa gaaggctgga caggctaaga agaagaaaca
     7561 acgcgtgcag ctaagcgact acaaagacca tgacggtgat tataaagatc atgacatcga
     7621 ttacaaggat gacgatgaca agtaaggatc cctccccccc ccctaacgtt actggccgaa
     7681 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     7741 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     7801 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     7861 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     7921 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     7981 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     8041 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     8101 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     8161 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataaggat
     8221 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8281 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8341 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8401 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8461 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8521 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8581 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8641 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     8701 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     8761 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     8821 aagcccggtg cctgacgccc atcgatagat cctaatcaac ctctggatta caaaatttgt
     8881 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8941 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     9001 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     9061 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9121 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9181 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9241 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9301 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9361 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9421 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     9481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9541 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     9601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9721 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     9781 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
     9841 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9901 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    10021 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    10081 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    10141 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    10201 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    10261 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
    10321 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10381 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10441 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10501 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10561 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10621 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10681 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10741 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10801 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10861 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10921 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10981 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    11041 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    11101 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    11161 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    11221 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    11281 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    11341 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    11401 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11461 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11521 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11581 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11641 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11701 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11761 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11821 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    11881 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
    11941 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    12001 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
    12061 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    12121 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    12181 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
    12241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    12301 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    12361 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    12421 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
