LOCUS       sequence-269027-       12426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen 
            LL
  TITLE     Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.
  JOURNAL   Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 
            10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19.
  PUBMED    31757757
REFERENCE   2  (bases 1 to 12426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.10.024"; journalName: "Mol Cell"; date: 
            "2019-12-19- 19"; volume: "76"; issue: "6"; pages: "981-997.e7"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-...
FEATURES             Location/Qualifiers
     source          1..12426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      6993..7002
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7473..7520
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7539..7604
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7611..8178
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7772..7789)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7999..8018
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8198..8797
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8198..8217)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8694..8714
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8816..9404
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8869..8889)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9287..9298)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9479..9712
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9831..9849)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9917..10021
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10022..10090,10091..10882)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10240..10259)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11053..11641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11542..11561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11795..11812
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12052..12073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12088..12093,12094..12111,12112..12118)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12126..12142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12131..12153
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12150..12166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12150..12166
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12216..12235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcaac atccccgctc
     3481 tggtggagaa tcagaagaag tacttcggca cctattccgt gatggccatg ctgaacgccc
     3541 agacagtgct ggatcacatc cagaaggtgg ccgacatcga gggcgagcag aacgagaaca
     3601 atgagaatct gtggtttcac cctgtgatga gccacctgta caacgccaag aatggctatg
     3661 ataagcagcc tgaaaagacc atgttcatca tcgagagact gcagtcctac ttcccatttc
     3721 tgaagatcat ggccgagaat cagcgcgagt actctaacgg caagtataag cagaatcggg
     3781 tggaggtgaa cagcaatgac atcttcgagg tgctgaagag agcctttggc gtgctgaaga
     3841 tgtacaggga tctgaccaac gcctacaaga catatgagga gaagctgaat gacggctgcg
     3901 agtttctgac cagcacagag cagcctctgt ccggcatgat caacaattac tataccgtgg
     3961 ccctgagaaa catgaatgag aggtacggct acaagacaga ggatctggcc ttcatccagg
     4021 acaagaggtt caagtttgtg aaggacgcct acggcaagaa gaagagccag gtgaacaccg
     4081 gcttctttct gtctctgcag gattataatg gcgacacaca gaagaagctg cacctgagcg
     4141 gagtgggaat cgccctgctg atctgcctgt ttctggataa gcagtacatc aacatcttcc
     4201 tgagcagact gccaatcttt agctcctata atgcccagtc cgaggagcgg agaatcatca
     4261 tccgcagctt cggcatcaac tccatcaagc tgccaaagga cagaatccac tccgagaagt
     4321 ctaacaagag cgtggccatg gatatgctga atgaggtgaa gaggtgtccc gacgagctgt
     4381 tcaccacact gtctgccgag aagcagagcc ggtttagaat catctccgac gatcacaacg
     4441 aggtgctgat gaagagatct agcgacaggt ttgtgcctct gctgctgcag tacatcgatt
     4501 atggcaagct gttcgaccac atccgctttc acgtgaacat gggcaagctg cggtacctgc
     4561 tgaaggccga taagacctgc atcgacggcc agacaagggt gcgcgtgatc gagcagccac
     4621 tgaatggatt cggccgcctg gaggaggcag agaccatgcg gaagcaggag aacggcacat
     4681 tcggcaatag cggcatccgg atcagagatt ttgagaacat gaagcgggac gatgccaacc
     4741 ctgccaatta cccatatatc gtggacacct acacacacta tatcctggag aacaataagg
     4801 tggagatgtt tatcaatgat aaggaggact ccgccccact gctgcccgtg atcgaggacg
     4861 atagatacgt ggtgaagacc atcccaagct gtaggatgtc cacactggag atccccgcca
     4921 tggccttcca catgttcctg tttggctcta agaagaccga gaagctgatc gtggacgtgc
     4981 acaaccggta taagagactg ttccaggcca tgcagaagga ggaggtgaca gccgagaata
     5041 tcgcctcttt tggcatcgcc gagagcgatc tgccccagaa gatcctggac ctgatctccg
     5101 gcaacgccca cggcaaggat gtggacgcct tcatccgcct gaccgtggac gatatgctga
     5161 ccgatacaga gaggcgcatc aagcggttta aggacgatcg caagtccatc cggtctgccg
     5221 acaataagat gggcaagcgc ggcttcaagc agatctctac cggcaagctg gccgatttcc
     5281 tggccaagga catcgtgctg tttcagccaa gcgtgaacga tggcgagaat aagatcacag
     5341 gcctgaacta ccggatcatg cagagcgcca tcgccgtgta cgactccggc gacgattatg
     5401 aggccaagca gcagttcaag ctgatgtttg agaaggccag actgatcggc aagggcacca
     5461 cagagcccca ccctttcctg tacaaggtgt ttgcaaggtc catcccagca aacgcagtgg
     5521 agttctacga gcggtacctg atcgagcgga agttttatct gaccggcctg tctaacgaga
     5581 tcaagaaggg caatcgcgtg gatgtgccct tcatccggag agaccagaat aagtggaaga
     5641 cccctgccat gaagacactg ggcagaatct acagcgagga tctgcccgtg gagctgccta
     5701 ggcagatgtt tgacaacgag atcaagtctc acctgaagag cctgcctcag atggagggca
     5761 tcgacttcaa caatgccaat gtgacctacc tgatcgccga gtatatgaag cgggtgctgg
     5821 acgatgactt ccagaccttc taccagtgga acaggaatta ccgctatatg gatatgctga
     5881 agggcgagta tgacagaaag ggctctctgc agcactgctt caccagcgtg gaggagaggg
     5941 agggactgtg gaaggagagg gcatccagaa cagagaggta ccgcaagcag gcctccaaca
     6001 agatcaggtc taatcgccag atgcggaacg cctcctctga ggagatcgag accatcctgg
     6061 ataagagact gtctaacagc aggaatgagt accagaagag cgagaaagtg atcaggcgct
     6121 atcgcgtgca ggacgccctg ctgttcctgc tggccaagaa gaccctgaca gagctggccg
     6181 atttcgacgg cgagcggttt aagctgaagg agatcatgcc agatgccgag aagggcatcc
     6241 tgtccgagat catgcccatg tctttcacat ttgagaaggg cggcaagaag tacaccatca
     6301 caagcgaggg catgaagctg aagaactatg gcgatttctt tgtgctggcc tccgacaaga
     6361 gaatcggcaa tctgctggag ctggtgggct ccgatatcgt gtctaaggag gacatcatgg
     6421 aggagtttaa caagtacgat cagtgtaggc ccgagatcag ctccatcgtg ttcaatctgg
     6481 agaagtgggc ctttgacacc tatcctgagc tgtccgccag agtggatagg gaggagaagg
     6541 tggacttcaa gtctatcctg aagatcctgc tgaacaataa gaacatcaat aaggagcagt
     6601 ctgacatcct gcgcaagatc cggaacgcct ttgatgccaa caattaccct gacaagggcg
     6661 tggtggagat caaggccctg ccagagatcg ccatgagcat caagaaggcc ttcggcgagt
     6721 atgccatcat gaagtctaga gtgtccaagg gcgaagagga caacatggcc agcctgcctg
     6781 ccacccacga gctgcacatc ttcggcagca tcaacggcgt ggacttcgac atggtgggac
     6841 agggcaccgg caaccccaac gacggctacg aggaactgaa cctgaagtcc acaaagggcg
     6901 acctgcagtt cagcccctgg attctggtgc cccacatcgg ctacggcttc caccagtacc
     6961 tgccctaccc cgacggcatg agcccttttc aggccgccat ggtggatggc agcggctacc
     7021 aggtgcaccg gaccatgcag tttgaggacg gcgccagcct gaccgtgaac taccggtaca
     7081 catacgaggg cagccacatc aagggcgagg cccaagtgaa gggcacaggc tttccagccg
     7141 acggccccgt gatgaccaat agcctgacag ccgccgactg gtgcagaagc aagaaaacct
     7201 accccaatga caagaccatc atcagcacct tcaagtggtc ctacaccacc ggcaatggca
     7261 agcggtacag aagcaccgcc cggaccacct acaccttcgc caaacccatg gccgccaact
     7321 acctgaagaa ccagcctatg tacgtgttcc gcaagaccga gctgaagcac tccaagacag
     7381 aactgaactt caaagagtgg cagaaagcct tcaccgacgt gatgggcatg gacgagctgt
     7441 acaagtccgg actcagatct tgcctacata tgaagcgacc tgccgccaca aagaaggctg
     7501 gacaggctaa gaagaagaaa caacgcgtgc agctaagcga ctacaaagac catgacggtg
     7561 attataaaga tcatgacatc gattacaagg atgacgatga caagtaagga tccctccccc
     7621 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     7681 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     7741 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     7801 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     7861 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     7921 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     7981 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     8041 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     8101 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     8161 tgaaaaacac gatgataagg atccaccgga gcttaccatg accgagtaca agcccacggt
     8221 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     8281 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     8341 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     8401 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     8461 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     8521 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     8581 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     8641 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     8701 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     8761 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccatcgatag atcctaatca
     8821 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     8881 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     8941 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     9001 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     9061 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     9121 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     9181 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     9241 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     9301 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     9361 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     9421 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     9481 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     9541 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9601 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9661 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     9721 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     9781 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     9841 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     9901 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     9961 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    10021 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    10081 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    10141 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    10201 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    10261 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    10321 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    10381 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    10441 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    10501 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    10561 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    10621 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    10681 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    10741 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    10801 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    10861 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    10921 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    10981 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    11041 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    11101 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    11161 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    11221 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    11281 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    11341 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    11401 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    11461 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    11521 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    11581 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    11641 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    11701 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    11761 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    11821 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
    11881 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    11941 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
    12001 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    12061 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    12121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
    12181 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    12241 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    12301 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    12361 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    12421 agtagt
//
