LOCUS       sequence-269043-         254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 engineering of the HO endonuclease gene with 
            nourseothricin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Barre B, Hallin J, Yue JX, Persson K, Mikhalev E, Irizar A, Holt S, 
            Thompson D, Molin M, Warringer J, Liti G
  TITLE     Intragenic repeat expansion in the cell wall protein gene HPF1 
            controls yeast chronological aging.
  JOURNAL   Genome Res. 2020 Apr 10. pii: gr.253351.119. doi: 
            10.1101/gr.253351.119.
  PUBMED    32277013
REFERENCE   2  (bases 1 to 254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2020 Apr 10. pii: gr.253351.119. doi: 10.1101/gr.253351.119."
COMMENT     Sequence Label: pL90-Nat-2xHO
FEATURES             Location/Qualifiers
     source          1..254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           21..57
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        78..153
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           158..225
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
ORIGIN
        1 ggacacagcg caaatcttta ctgatgagtc cgtgaggacg aaacgagtaa gctcgtctaa
       61 agacatcgca aacgtcagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      121 tatcaacttg aaaaagtggc accgagtcgg tgcttttggc cggcatggtc ccagcctcct
      181 cgctggcgcc ggctgggcaa catgcttcgg catggcgaat gggacacagg ccccttttcc
      241 tttgtcgata tcat
//
